AB117504

DyLight® 650 Anti-6X His tag® antibody [AD1.1.10]

Name: DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504) | Abcam
Brand: Abcam Limited
SKU: AB117504
Price: 495 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(10 Publications)

Anti-6X His tag® antibody [AD1.1.10] - DyLight® 650 conjugated (ab117504) is a mouse monoclonal antibody detecting 6X His tag® in Flow Cytometry, ICC/IF.

View Alternative Names

6 His epitope tag, HHHHHH epitope tag, HHHHHH tag, Hexa His tag, Polyhistidine Tag, 6x his, 6x-HIS

1 Images

Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (AB117504)

ICC/IF

AbReview57121****

Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (AB117504)

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells transfected with His-tagged actin labeling 6X His tag^® with ab117504 at 1/1000 dilution. The cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton-X100 in PBS. The cells were blocked with 5% BSA for 12 hours at 4°C, followed by incubation with Anti-6X His tag^® antibody [AD1.1.10] (DyLight® 650) (ab117504) for 2 hours at 25°C.

This image is courtesy of an anonymous abreview.

Unconjugated

Anti-6X His tag® antibody [AD1.1.10]
Alkaline Phosphatase

Alkaline Phosphatase Anti-6X His tag® antibody [AD1.1.10]
518 DyLight® 488

DyLight® 488 Anti-6X His tag® antibody [AD1.1.10]
576 DyLight® 550

DyLight® 550 Anti-6X His tag® antibody [AD1.1.10]
HRP

HRP Anti-6X His tag® antibody [AD1.1.10]
578 PE

PE Anti-6X His tag® antibody [AD1.1.10]
675 PerCP

PerCP Anti-6X His tag® antibody [AD1.1.10]
657 SureLight® APC

SureLight® APC Anti-6X His tag® antibody [AD1.1.10]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

AD1.1.10

Isotype

IgG1

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Carrier free

Yes

Applications

ICC/IF, Flow Cyt

applications

Specificity

ab117504 reacts to recombinant proteins containing the 6X His tag® fused to either the amino or carboxy terminus.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/5000", "ICCIF-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (ab117504) is a mouse monoclonal antibody and is validated for use in Flow Cytometry (Flow Cyt), Immunocytochemistry/immunofluorescence (ICC/IF). Tagged protein detection
6X His tag® antibodies are used to visualize proteins labelled with this tag in a variety of applications.

Related products
Antibody clone AD1.1.10 is also available pre-conjugated to a variety of labels for your convenience – Anti-6X His tag® DyLight® 650 [AD1.1.10] (ab117504).

Other related products
We have a range of other formats of antibody clone [AD1.1.10] also available for your convenience: ab15149, PE - ab72467, SureLight® APC - ab72579, Alkaline Phosphatase - ab81652, PerCP - ab117496, Carrier free - ab117504, DyLight® 550 - ab117507, DyLight® 488 - ab117512, HRP - ab178563, Carrier free - ab307236

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 8.2 Constituents: 99% Tris buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

The product can be stored for up to 12 months

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The 6X His tag is a sequence commonly used for protein purification detection and immobilization. It consists of six histidine residues that bind to metal ions like nickel or cobalt. This makes it a highly efficient tag for affinity chromatography. His tag antibodies such as anti-His HRP facilitate the detection of His-tagged proteins in various assays. Being a small tag with a typical molecular weight of about 0.84 kDa it minimally interferes with protein function. Many proteins expressed in E. coli and other expression systems carry the 6X His tag for ease of purification and analysis.

Biological function summary

Proteins tagged with the 6X His tag often play roles in studies involving recombinant proteins. The tag itself does not alter the biological function of the target protein. It is not part of any natural protein complex but aids in the study of such complexes. Researchers use His tag antibodies like anti-His to specifically target and study these tagged proteins without cross-reactivity to other proteins.

Pathways

The incorporation of the 6X His tag into proteins allows them to be studied more efficiently in various biological pathways. It can be part of pathways like signal transduction or metabolic pathways. When tagged proteins interact with other molecules such as kinases or receptors researchers can track these interactions using the tag and anti-Histidine antibodies. This technique informs on proteins that are hard to purify due to low abundance or instability.

The 6X His tag itself does not have direct implications. However proteins of interest in disease models often carry the tag which allows researchers to purify and study them in detail. For example cancer-related proteins may be expressed with a His tag to understand their role in tumorigenesis. Similarly proteins involved in metabolic disorders could be tagged which helps in analyzing specific interactions or functions disrupted in diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information 6X His tag®

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:7889 PubMed40849430

2025

Mesothelin-directed protein-drug conjugates for mesothelin-low solid tumor therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Wang,Jiayao Yan,Lin Li,Tianran Chen,Jingyi Guo,Lei Lei,Siwen Wu,Lijing Zhu,Xiaotong Chen,Baorui Liu

iScience 27:109558 PubMed38650982

2024

Haemolysins are essential to the pathogenicity of deep-sea .

Applications

Unspecified application

Species

Unspecified reactive species

Yujian Wang,Jingchang Luo,Yan Zhao,Jian Zhang,Xiaolu Guan,Li Sun

International journal of medical sciences 18:207-215 PubMed33390789

2021

Reduction of pl-CSA through ChSy-2 knockout inhibits tumorigenesis and metastasis of choriocarcinoma in JEG3 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juzuo Zhang,Zhilong Chen,Baobei Wang,Jie Chen,Tianxia Xiao,Jian V Zhang,Shiling Chen,Xiujun Fan

Journal for immunotherapy of cancer 8: PubMed33203663

2020

Nuclear imaging-guided PD-L1 blockade therapy increases effectiveness of cancer immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Hannan Gao,Yue Wu,Jiyun Shi,Xin Zhang,Tianyu Liu,Biao Hu,Bing Jia,Yakun Wan,Zhaofei Liu,Fan Wang

Cell 180:323-339.e19 PubMed31928845

2020

Structural Basis of Teneurin-Latrophilin Interaction in Repulsive Guidance of Migrating Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Del Toro,Maria A Carrasquero-Ordaz,Amy Chu,Tobias Ruff,Meriam Shahin,Verity A Jackson,Matthieu Chavent,Miguel Berbeira-Santana,Goenuel Seyit-Bremer,Sara Brignani,Rainer Kaufmann,Edward Lowe,Rüdiger Klein,Elena Seiradake

eLife 8: PubMed31282865

2019

Quantitative mapping of protein-peptide affinity landscapes using spectrally encoded beads.

Applications

Unspecified application

Species

Unspecified reactive species

Huy Quoc Nguyen,Jagoree Roy,Björn Harink,Nikhil P Damle,Naomi R Latorraca,Brian C Baxter,Kara Brower,Scott A Longwell,Tanja Kortemme,Kurt S Thorn,Martha S Cyert,Polly Morrell Fordyce

PloS one 14:e0203725 PubMed30901328

2019

An open-source software analysis package for Microspheres with Ratiometric Barcode Lanthanide Encoding (MRBLEs).

Applications

Unspecified application

Species

Unspecified reactive species

Björn Harink,Huy Nguyen,Kurt Thorn,Polly Fordyce

Clinical cancer research : an official journal of the American Association for Cancer Research 25:1774-1785 PubMed30373750

2018

A Novel Engineered Small Protein for Positron Emission Tomography Imaging of Human Programmed Death Ligand-1: Validation in Mouse Models and Human Cancer Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Arutselvan Natarajan,Chirag B Patel,Sindhuja Ramakrishnan,Paramjyot S Panesar,Steven R Long,Sanjiv S Gambhir

Proceedings of the National Academy of Sciences of 113:E4847-56 PubMed27482114

2016

CCN1/CYR61-mediated meticulous patrolling by Ly6Clow monocytes fuels vascular inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Beat A Imhof,Stephane Jemelin,Romain Ballet,Christian Vesin,Marc Schapira,Melis Karaca,Yalin Emre

PloS one 10:e0145820 PubMed26713870

2015

Engineering an Anti-Transferrin Receptor ScFv for pH-Sensitive Binding Leads to Increased Intracellular Accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin J Tillotson,Loukas I Goulatis,Isabelle Parenti,Elizabeth Duxbury,Eric V Shusta

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

DyLight® 650 Anti-6X His tag® antibody [AD1.1.10]

Immunocytochemistry/ Immunofluorescence - DyLight® 650 Anti-6X His tag® antibody [AD1.1.10] (AB117504)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

Product promise