Mouse Monoclonal HA tag antibody - conjugated to DyLight® 650. Suitable for Flow Cyt, ICC/IF and reacts with Tag samples. Cited in 7 publications.
Images
![Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (AB117515), expandable thumbnail](https://content.abcam.com/products/images/dylight-650-ha-tag-antibody-16b12-ab117515--flow-cytometry-img55804.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (AB117515)")
Publications
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- Biomolecules 13:2023Engineering of a Bispecific Nanofitin with Immune Checkpoint Inhibitory Activity Conditioned by the Cross-Arm Binding to EGFR and PDL1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPerrine Jacquot,Javier Muñoz-Garcia,Maurine Fleury,Denis Cochonneau,Rémi Gaussin,Elise Enouf,Caroline Roze,Emilie Ollivier,Mathieu Cinier,Dominique HeymannPubMed 37189383
- Cell reports 33:1082492020Quantitative Proteomics Analysis of Lytic KSHV Infection in Human Endothelial Cells Reveals Targets of Viral Immune Modulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIldar Gabaev,James C Williamson,Thomas W M Crozier,Thomas F Schulz,Paul J LehnerPubMed 33053346
- Cell reports 31:1076912020Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMonica Nafria,Peter Keane,Elizabeth S Ng,Edouard G Stanley,Andrew G Elefanty,Constanze BoniferPubMed 32460028
- The Journal of cell biology 219:2020GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR ΔF508.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSafa Lucken-Ardjomande Häsler,Yvonne Vallis,Mathias Pasche,Harvey T McMahonPubMed 32344433
- eLife 9:2020Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Marelli et. al.PubMed 32292164
- Scientific reports 7:134482017LRRC25 Functions as an Inhibitor of NF-κB Signaling Pathway by Promoting p65/RelA for Autophagic Degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanchun Feng et. al.PubMed 29044191
- eLife 5:2016Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdward Jd Greenwood et. al.PubMed 27690223
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- DyLight® 650
- Storage buffer
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 95% Sodium phosphate, 0.58% Sodium chloride- Form
- Liquid
- Clonality
- Monoclonal
Reactivity data
Select an application| Flow Cyt | ICC/IF | |
|---|---|---|
| Tag | Tested | Expected |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Tag | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
3 products for Alternative Version
Target data
Alternative names
HA epitope tag, HA2, Hemagglutinin, Hemagglutinin HA1 chain, Hemagglutinin HA2 chain
Recommended products
Mouse Monoclonal HA tag antibody - conjugated to DyLight® 650. Suitable for Flow Cyt, ICC/IF and reacts with Tag samples. Cited in 7 publications.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Conjugation
- DyLight® 650
- Storage buffer
pH: 7.4
Preservative: 0.01% Sodium azide
Constituents: 95% Sodium phosphate, 0.58% Sodium chloride- Form
- Liquid
- Clonality
- Monoclonal
- Clone number
- 16B12
- Purification technique
- Affinity purification Protein G
- Specificity
ab117515 recognizes the Influenza hemagglutinin epitope (YPYDVPDYA).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- The product can be stored for up to 12 months
Notes
Conjugated to Dylight® 650 (ratio 1.3:): Visible absorption maxima 652 nm; Emission maximum 672 nm.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (ab117515), expandable thumbnail](https://content.abcam.com/products/images/dylight-650-ha-tag-antibody-16b12-ab117515--flow-cytometry-img55804.jpg "Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)")
This image is courtesy of an anonymous customer reviewFlow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (ab117515)
ab117515 staining 293T cells expressing a HA-tagged protein (intracellular) by Flow Cytometry. Cells were permeabilized and fixed. The sample was incubated with the primary antibody (1/200) for 30 minutes at 4°C.
Gating Strategy: Gated on FSC-SSC to exclude debris/dead cells.
Grey - unstained cells.
Red - Wild type 293T, stained.
Blue - 293T expressing HA-tagged protein, stained.
Downloads
Product protocols
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