AB117515

DyLight® 650 Anti-HA tag antibody [16B12]

Name: DyLight® 650 Anti-HA tag antibody [16B12] (ab117515) | Abcam
Brand: Abcam Limited
SKU: AB117515
Price: 420 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(7 Publications)

Mouse Monoclonal HA tag antibody - conjugated to DyLight® 650. Suitable for Flow Cyt, ICC/IF and reacts with Tag samples. Cited in 7 publications.

1 Images

Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (AB117515)

Flow Cyt

AbReview47985****

Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (AB117515)

ab117515 staining 293T cells expressing a HA-tagged protein (intracellular) by Flow Cytometry. Cells were permeabilized and fixed. The sample was incubated with the primary antibody (1/200) for 30 minutes at 4°C.

Gating Strategy : Gated on FSC-SSC to exclude debris/dead cells.

Grey - unstained cells.

Red - Wild type 293T, stained.

Blue - 293T expressing HA-tagged protein, stained.

This image is courtesy of an anonymous Abreview

Alkaline Phosphatase

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

16B12

Isotype

IgG1

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Carrier free

Applications

Flow Cyt, ICC/IF

applications

Specificity

ab117515 recognizes the Influenza hemagglutinin epitope (YPYDVPDYA).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500 - 1/5000", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Conjugated to Dylight^® 650 (ratio 1.3:): Visible absorption maxima 652 nm; Emission maximum 672 nm.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.01% Sodium azide Constituents: 95% Sodium phosphate, 0.58% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

The product can be stored for up to 12 months

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information HA tag

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 13: PubMed37189383

2023

Engineering of a Bispecific Nanofitin with Immune Checkpoint Inhibitory Activity Conditioned by the Cross-Arm Binding to EGFR and PDL1.

Applications

Unspecified application

Species

Unspecified reactive species

Perrine Jacquot,Javier Muñoz-Garcia,Maurine Fleury,Denis Cochonneau,Rémi Gaussin,Elise Enouf,Caroline Roze,Emilie Ollivier,Mathieu Cinier,Dominique Heymann

Cell reports 33:108249 PubMed33053346

2020

Quantitative Proteomics Analysis of Lytic KSHV Infection in Human Endothelial Cells Reveals Targets of Viral Immune Modulation.

Applications

Unspecified application

Species

Unspecified reactive species

Ildar Gabaev,James C Williamson,Thomas W M Crozier,Thomas F Schulz,Paul J Lehner

Cell reports 31:107691 PubMed32460028

2020

Expression of RUNX1-ETO Rapidly Alters the Chromatin Landscape and Growth of Early Human Myeloid Precursor Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Monica Nafria,Peter Keane,Elizabeth S Ng,Edouard G Stanley,Andrew G Elefanty,Constanze Bonifer

The Journal of cell biology 219: PubMed32344433

2020

GRAF2, WDR44, and MICAL1 mediate Rab8/10/11-dependent export of E-cadherin, MMP14, and CFTR ΔF508.

Applications

Unspecified application

Species

Unspecified reactive species

Safa Lucken-Ardjomande Häsler,Yvonne Vallis,Mathias Pasche,Harvey T McMahon

eLife 9: PubMed32292164

2020

Antagonism of PP2A is an independent and conserved function of HIV-1 Vif and causes cell cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Marelli,James C Williamson,Anna V Protasio,Adi Naamati,Edward Jd Greenwood,Janet E Deane,Paul J Lehner,Nicholas J Matheson

Scientific reports 7:13448 PubMed29044191

2017

LRRC25 Functions as an Inhibitor of NF-κB Signaling Pathway by Promoting p65/RelA for Autophagic Degradation.

Applications

Unspecified application

Species

Unspecified reactive species

Yanchun Feng,Tianhao Duan,Yang Du,Shouheng Jin,Mingjun Wang,Jun Cui,Rong-Fu Wang

eLife 5: PubMed27690223

2016

Temporal proteomic analysis of HIV infection reveals remodelling of the host phosphoproteome by lentiviral Vif variants.

Applications

Unspecified application

Species

Unspecified reactive species

Edward Jd Greenwood,Nicholas J Matheson,Kim Wals,Dick Jh van den Boomen,Robin Antrobus,James C Williamson,Paul J Lehner

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

DyLight® 650 Anti-HA tag antibody [16B12]

Flow Cytometry - DyLight® 650 Anti-HA tag antibody [16B12] (AB117515)

Related conjugates and formulations (5)

Key facts

Host species

Clonality

Clone number

Isotype

Conjugation

Excitation/Emission

Carrier free

Applications

Specificity

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (7)

Product promise