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Rabbit Recombinant Monoclonal Dynamin 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

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Images

Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322), expandable thumbnail
  • Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322), expandable thumbnail
  • Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBIHC-P
Human
Not recommended
Tested
Tested
Mouse
Not recommended
Tested
Tested
Rat
Not recommended
Tested
Expected

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission and participates in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis as well as rapid endocytosis (RE) (PubMed:15703209, PubMed:20428113, PubMed:29668686, PubMed:8101525, PubMed:8910402, PubMed:9362482). Associates to the membrane, through lipid binding, and self-assembles into rings and stacks of interconnected rings through oligomerization to form a helical polymer around the vesicle membrane leading to constriction of invaginated coated pits around their necks (PubMed:30069048, PubMed:7877694, PubMed:9922133). Self-assembly of the helical polymer induces membrane tubules narrowing until the polymer reaches a length sufficient to trigger GTP hydrolysis (PubMed:19084269). Depending on the curvature imposed on the tubules, membrane detachment from the helical polymer upon GTP hydrolysis can cause spontaneous hemifission followed by complete fission (PubMed:19084269). May play a role in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells through its activation by dephosphorylation via the signaling downstream of EGFR (PubMed:29668686). Controls vesicle size at a step before fission, during formation of membrane pits, at hippocampal synapses (By similarity). Controls plastic adaptation of the synaptic vesicle recycling machinery to high levels of activity (By similarity). Mediates rapid endocytosis (RE), a Ca(2+)-dependent and clathrin- and K(+)-independent process in chromaffin cells (By similarity). Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP (By similarity). Through its interaction with DNAJC6, acts during the early steps of clathrin-coated vesicle (CCV) formation (PubMed:12791276).

Alternative names

DNM, DNM1, Dynamin-1, Dynamin, Dynamin I

Recommended products

Rabbit Recombinant Monoclonal Dynamin 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EP801Y
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab247322 is the carrier-free version of Anti-Dynamin 1 antibody [EP801Y] ab52611.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Dynamin 1 also known as DNM1 is a GTPase with a molecular mass of approximately 96 kDa. It plays a mechanical role in membrane fission during endocytosis. Dynamin 1 is primarily expressed in neuronal tissues where it participates in the formation and release of synaptic vesicles. Its ability to hydrolyze GTP gives it the mechanical force needed to constrict and sever membranes which is vital for efficient synaptic vesicle recycling in neurons.

Biological function summary

Dynamin 1 helps regulate synaptic transmission by controlling vesicular traffic in neurons. As part of a larger protein complex it interacts with various proteins to mediate endocytosis specifically in presynaptic nerve terminals. These interactions ensure that synaptic vesicles are recycled rapidly allowing neurons to maintain communication across synapses. Disruptions in dynamin 1 function can impair synaptic vesicle recycling affecting neurotransmission efficiency.

Pathways

Dynamin 1 is essential in the clathrin-mediated endocytic pathway. This pathway is a major route for internalizing synaptic vesicles and involves cooperation with other proteins such as clathrin and adaptor proteins. Additionally dynamin 1 participates in the actin cytoskeleton dynamics linking it with actin-associated proteins. These pathways highlight its contributions to maintaining synaptic vesicle endocytosis and neurite outgrowth processes.

Associated diseases and disorders

Alterations in dynamin 1 function are linked to neurological disorders including epilepsy and Huntington's disease. In epilepsy improper functioning of dynamin 1 disrupts synaptic vesicle recycling leading to imbalances in neurotransmitter release. Connections exist between dynamin 1 and huntingtin where their interactions in vesicular trafficking are important for neuronal stability. Malfunctions in these processes often result in neurodegenerative symptoms emphasizing dynamin 1's involvement in sustaining neural health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322), expandable thumbnail

    Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)

    This data was developed using the same antibody in a different buffer formulation (Anti-Dynamin 1 antibody [EP801Y] ab52611).

    Anti-Dynamin 1 antibody [EP801Y] ab52611 was shown to react with DNM1 in wild-type U-2 OS cells in Western blot with loss of signal observed in a DNM1 knockout cell line. Wild-type U-2 OS and DNM1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with Anti-Dynamin 1 antibody [EP801Y] ab52611 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

    All lanes: Western blot - Anti-Dynamin 1 antibody [EP801Y] (Anti-Dynamin 1 antibody [EP801Y] ab52611) at 1/1000 dilution

    Lane 1: Wild-type U-2 OS cell lysate at 20 µg

    Lane 2: DNM1 knockout U-2 OS cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 97 kDa

  • Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322), expandable thumbnail

    Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)

    This data was developed using Anti-Dynamin 1 antibody [EP801Y] ab52611, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Dynamin 1 antibody [EP801Y] (Anti-Dynamin 1 antibody [EP801Y] ab52611) at 1/10000 dilution

    All lanes: SH-SY5Y cell lysate at 10 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

    Predicted band size: 97 kDa

    Observed band size: 97 kDa

  • Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322), expandable thumbnail

    Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)

    This data was developed using Anti-Dynamin 1 antibody [EP801Y] ab52611, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Dynamin 1 antibody [EP801Y] (Anti-Dynamin 1 antibody [EP801Y] ab52611) at 1/2000 dilution

    Lane 1: C6 cell lysate at 20 µg

    Lane 2: NIH/3T3 cell lysate at 20 µg

    Secondary

    All lanes: HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 97 kDa

    Observed band size: 97 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)

    This data was developed using Anti-Dynamin 1 antibody [EP801Y] ab52611, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified Anti-Dynamin 1 antibody [EP801Y] ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)

    This data was developed using Anti-Dynamin 1 antibody [EP801Y] ab52611, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human cerebral cortex with purified Anti-Dynamin 1 antibody [EP801Y] ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

  • Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322), expandable thumbnail

    Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)

    This data was developed using Anti-Dynamin 1 antibody [EP801Y] ab52611, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-Dynamin 1 antibody [EP801Y] (Anti-Dynamin 1 antibody [EP801Y] ab52611) at 1/2000 dilution

    All lanes: 3T3 cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP antibody at 1/2000 dilution

    Predicted band size: 84 kDa, 97 kDa

    Observed band size: 88 kDa, 97 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (ab247322)

    This data was developed using Anti-Dynamin 1 antibody [EP801Y] ab52611, the same antibody clone in a different buffer formulation.Unpurified Anti-Dynamin 1 antibody [EP801Y] ab52611 at 1/100-1/250 dilution staining human brain tissue; paraffin embedded.

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