AB247322

Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal Dynamin 1 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

View Alternative Names

DNM, DNM1, Dynamin-1, Dynamin, Dynamin I

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

This data was developed using ab52611, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded human cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

This data was developed using ab52611, the same antibody clone in a different buffer formulation.

Unpurified ab52611 at 1/100-1/250 dilution staining human brain tissue; paraffin embedded.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

This data was developed using ab52611, the same antibody clone in a different buffer formulation.Immunohistochemical staining of paraffin embedded mouse cerebral cortex with purified ab52611 at a working dilution of 1/50. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

Collaborator

Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

This data was developed using the same antibody in a different buffer formulation (ab52611).

ab52611 was shown to react with DNM1 in wild-type U-2 OS cells in Western blot with loss of signal observed in a DNM1 knockout cell line. Wild-type U-2 OS and DNM1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab52611 overnight at 4 °C at a 1/1000 dilution. Blots were incubated with goat anti-rabbit HRP secondary antibodies at 0.2ug/mL before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.

All lanes:

Western blot - Anti-Dynamin 1 antibody [EP801Y] (<a href='/en-us/products/primary-antibodies/dynamin-1-antibody-ep801y-ab52611'>ab52611</a>) at 1/1000 dilution

Lane 1:

Wild-type U-2 OS cell lysate at 20 µg

Lane 2:

DNM1 knockout U-2 OS cell lysate at 20 µg

Predicted band size: 97 kDa

false

Lab

Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

This data was developed using ab52611, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Dynamin 1 antibody [EP801Y] (<a href='/en-us/products/primary-antibodies/dynamin-1-antibody-ep801y-ab52611'>ab52611</a>) at 1/10000 dilution

All lanes:

SH-SY5Y cell lysate at 10 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/50000 dilution

Predicted band size: 97 kDa

Observed band size: 97 kDa

false

Lab

Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

This data was developed using ab52611, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Dynamin 1 antibody [EP801Y] (<a href='/en-us/products/primary-antibodies/dynamin-1-antibody-ep801y-ab52611'>ab52611</a>) at 1/2000 dilution

Lane 1:

C6 cell lysate at 20 µg

Lane 2:

NIH/3T3 cell lysate at 20 µg

Secondary

All lanes:

HRP goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 97 kDa

Observed band size: 97 kDa

false

Unknown

Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

This data was developed using ab52611, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Dynamin 1 antibody [EP801Y] (<a href='/en-us/products/primary-antibodies/dynamin-1-antibody-ep801y-ab52611'>ab52611</a>) at 1/2000 dilution

All lanes:

3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 84 kDa,97 kDa

Observed band size: 88 kDa,97 kDa

false

Unconjugated

Anti-Dynamin 1 antibody [EP801Y]
HRP

HRP Anti-Dynamin 1 antibody [EP801Y]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP801Y

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab247322 is the carrier-free version of ab52611.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dynamin 1 also known as DNM1 is a GTPase with a molecular mass of approximately 96 kDa. It plays a mechanical role in membrane fission during endocytosis. Dynamin 1 is primarily expressed in neuronal tissues where it participates in the formation and release of synaptic vesicles. Its ability to hydrolyze GTP gives it the mechanical force needed to constrict and sever membranes which is vital for efficient synaptic vesicle recycling in neurons.

Biological function summary

Dynamin 1 helps regulate synaptic transmission by controlling vesicular traffic in neurons. As part of a larger protein complex it interacts with various proteins to mediate endocytosis specifically in presynaptic nerve terminals. These interactions ensure that synaptic vesicles are recycled rapidly allowing neurons to maintain communication across synapses. Disruptions in dynamin 1 function can impair synaptic vesicle recycling affecting neurotransmission efficiency.

Pathways

Dynamin 1 is essential in the clathrin-mediated endocytic pathway. This pathway is a major route for internalizing synaptic vesicles and involves cooperation with other proteins such as clathrin and adaptor proteins. Additionally dynamin 1 participates in the actin cytoskeleton dynamics linking it with actin-associated proteins. These pathways highlight its contributions to maintaining synaptic vesicle endocytosis and neurite outgrowth processes.

Alterations in dynamin 1 function are linked to neurological disorders including epilepsy and Huntington's disease. In epilepsy improper functioning of dynamin 1 disrupts synaptic vesicle recycling leading to imbalances in neurotransmitter release. Connections exist between dynamin 1 and huntingtin where their interactions in vesicular trafficking are important for neuronal stability. Malfunctions in these processes often result in neurodegenerative symptoms emphasizing dynamin 1's involvement in sustaining neural health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolysis of GTP and utilizes this energy to mediate vesicle scission and participates in many forms of endocytosis, such as clathrin-mediated endocytosis or synaptic vesicle endocytosis as well as rapid endocytosis (RE) (PubMed : 15703209, PubMed : 20428113, PubMed : 29668686, PubMed : 8101525, PubMed : 8910402, PubMed : 9362482). Associates to the membrane, through lipid binding, and self-assembles into rings and stacks of interconnected rings through oligomerization to form a helical polymer around the vesicle membrane leading to constriction of invaginated coated pits around their necks (PubMed : 30069048, PubMed : 7877694, PubMed : 9922133). Self-assembly of the helical polymer induces membrane tubules narrowing until the polymer reaches a length sufficient to trigger GTP hydrolysis (PubMed : 19084269). Depending on the curvature imposed on the tubules, membrane detachment from the helical polymer upon GTP hydrolysis can cause spontaneous hemifission followed by complete fission (PubMed : 19084269). May play a role in regulating early stages of clathrin-mediated endocytosis in non-neuronal cells through its activation by dephosphorylation via the signaling downstream of EGFR (PubMed : 29668686). Controls vesicle size at a step before fission, during formation of membrane pits, at hippocampal synapses (By similarity). Controls plastic adaptation of the synaptic vesicle recycling machinery to high levels of activity (By similarity). Mediates rapid endocytosis (RE), a Ca(2+)-dependent and clathrin- and K(+)-independent process in chromaffin cells (By similarity). Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP (By similarity). Through its interaction with DNAJC6, acts during the early steps of clathrin-coated vesicle (CCV) formation (PubMed : 12791276).

See full target information DNM1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 18:477 PubMed37393232

2023

CircTBX5 knockdown modulates the miR-558/MyD88 axis to alleviate IL-1β-induced inflammation, apoptosis and extracellular matrix degradation in chondrocytes via inactivating the NF-κB signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Hongjie Mu,Qiaoyi Cui,Peng Yu,Tong Liu,Tao Wang,Lin Sheng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

Western blot - Anti-Dynamin 1 antibody [EP801Y] - BSA and Azide free (AB247322)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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