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AB134925

Anti-Dynamin 3 antibody [EPR9055]

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(2 Publications)

Rabbit Recombinant Monoclonal Dynamin 3 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

KIAA0820, DNM3, Dynamin-3, T-dynamin

1 Images
Western blot - Anti-Dynamin 3 antibody [EPR9055] (AB134925)
  • WB

Unknown

Western blot - Anti-Dynamin 3 antibody [EPR9055] (AB134925)

All lanes:

Western blot - Anti-Dynamin 3 antibody [EPR9055] (ab134925) at 1/1000 dilution

All lanes:

Fetal brain lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 97 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9055

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dynamin 3 also known as DNM3 is a GTPase enzyme involved in the regulation of membrane fission events. It belongs to the dynamin family which also includes dynamin 1 and dynamin 2. Dynamin 3 has a molecular weight of approximately 97 kDa. This protein expresses in neurons where it plays a critical role in synaptic vesicle endocytosis. Additionally its expression can be found in the testes and lungs indicating a role in cellular events specific to these tissues.
Biological function summary

Dynamin 3 functions as part of multi-protein complexes that mediate membrane trafficking. In neurons it partners with other proteins to ensure precise timing of vesicle scission during synaptic transmission. Its ability to hydrolyze GTP provides the force necessary for membrane constriction and this activity is essential for maintaining normal neurotransmitter release. Dynamin 3 interacts with proteins including endophilin and amphiphysin which assist in the binding to membrane lipids ensuring proper recruitment to the sites of vesicle budding.

Pathways

Cellular events involving dynamin 3 are integral to synaptic vesicle recycling and endocytosis. Dynamin 3 functions within the clathrin-mediated endocytosis pathway which ensures the recycling of synaptic vesicles following neurotransmitter release. Within this pathway it collaborates extensively with clathrin and adaptin proteins playing a critical role in the disassembly of the clathrin-coated pits. Dynamin 3's activity is closely coordinated with other members of the dynamin protein family such as dynamin 1 which shares overlapping roles in similar cellular contexts.

Dysregulation of dynamin 3 is associated with certain neurodegenerative disorders such as Charcot-Marie-Tooth disease and epilepsy. Alterations in its function can disrupt normal synaptic vesicle trafficking leading to impaired synaptic transmission contributing to the pathology of these conditions. The interaction of dynamin 3 with proteins such as myelin protein zero (related to Charcot-Marie-Tooth disease) and voltage-gated ion channels (related to epilepsy) highlights its significant role in the nervous system where its proper function is needed for cognitive and motor abilities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis (By similarity).
See full target information DNM3
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Nature cancer 3:961-975 PubMed35982179

2022

Single-cell analysis and functional characterization uncover the stem cell hierarchies and developmental origins of rhabdomyosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Wei,Qian Qin,Chuan Yan,Madeline N Hayes,Sara P Garcia,Haibin Xi,Daniel Do,Alexander H Jin,Tiffany C Eng,Karin M McCarthy,Abhinav Adhikari,Maristela L Onozato,Dimitrios Spentzos,Gunnlaugur P Neilsen,A John Iafrate,Leonard H Wexler,April D Pyle,Mario L Suvà,Filemon Dela Cruz,Luca Pinello,David M Langenau

Bioengineered 13:6208-6221 PubMed35200104

2022

Exosomal microRNA-23a-3p contributes to the progression of cholangiocarcinoma by interaction with Dynamin3.

Applications

Unspecified application

Species

Unspecified reactive species

Qingfeng Ni,Hai Zhang,Xiaoli Shi,Xiangcheng Li
View all publications
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