JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB248734

Anti-Dynamin 3 antibody [EPR9055] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal Dynamin 3 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

KIAA0820, DNM3, Dynamin-3, T-dynamin

1 Images
Western blot - Anti-Dynamin 3 antibody [EPR9055] - BSA and Azide free (AB248734)
  • WB

Unknown

Western blot - Anti-Dynamin 3 antibody [EPR9055] - BSA and Azide free (AB248734)

This data was developed using ab134925, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Dynamin 3 antibody [EPR9055] (<a href='/en-us/products/primary-antibodies/dynamin-3-antibody-epr9055-ab134925'>ab134925</a>) at 1/1000 dilution

All lanes:

Fetal brain lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 97 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9055

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab248734 is the carrier-free version of ab134925.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dynamin 3 also known as DNM3 is a GTPase enzyme involved in the regulation of membrane fission events. It belongs to the dynamin family which also includes dynamin 1 and dynamin 2. Dynamin 3 has a molecular weight of approximately 97 kDa. This protein expresses in neurons where it plays a critical role in synaptic vesicle endocytosis. Additionally its expression can be found in the testes and lungs indicating a role in cellular events specific to these tissues.
Biological function summary

Dynamin 3 functions as part of multi-protein complexes that mediate membrane trafficking. In neurons it partners with other proteins to ensure precise timing of vesicle scission during synaptic transmission. Its ability to hydrolyze GTP provides the force necessary for membrane constriction and this activity is essential for maintaining normal neurotransmitter release. Dynamin 3 interacts with proteins including endophilin and amphiphysin which assist in the binding to membrane lipids ensuring proper recruitment to the sites of vesicle budding.

Pathways

Cellular events involving dynamin 3 are integral to synaptic vesicle recycling and endocytosis. Dynamin 3 functions within the clathrin-mediated endocytosis pathway which ensures the recycling of synaptic vesicles following neurotransmitter release. Within this pathway it collaborates extensively with clathrin and adaptin proteins playing a critical role in the disassembly of the clathrin-coated pits. Dynamin 3's activity is closely coordinated with other members of the dynamin protein family such as dynamin 1 which shares overlapping roles in similar cellular contexts.

Dysregulation of dynamin 3 is associated with certain neurodegenerative disorders such as Charcot-Marie-Tooth disease and epilepsy. Alterations in its function can disrupt normal synaptic vesicle trafficking leading to impaired synaptic transmission contributing to the pathology of these conditions. The interaction of dynamin 3 with proteins such as myelin protein zero (related to Charcot-Marie-Tooth disease) and voltage-gated ion channels (related to epilepsy) highlights its significant role in the nervous system where its proper function is needed for cognitive and motor abilities.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Microtubule-associated force-producing protein involved in producing microtubule bundles and able to bind and hydrolyze GTP. Most probably involved in vesicular trafficking processes, in particular endocytosis (By similarity).
See full target information DNM3
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Primary Antibodies

AB32503

Anti-Bax antibody [E63]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [E63] (AB32503)

  • 4
  • 12
Primary Antibodies

AB40772

Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB40772)

  • 4
  • 23

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com