Rabbit Recombinant Monoclonal DYNC1I2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples.
Images
![Western blot - Anti-DYNC1I2 antibody [EPR11242] (AB167399), expandable thumbnail](https://content.abcam.com/products/images/dync1i2-antibody-epr11242-ab167399--western-blot-img99372.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-DYNC1I2 antibody [EPR11242] (AB167399)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNC1I2 antibody [EPR11242] (AB167399), expandable thumbnail](https://content.abcam.com/products/images/dync1i2-antibody-epr11242-ab167399--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42633.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNC1I2 antibody [EPR11242] (AB167399)")
![Immunoprecipitation - Anti-DYNC1I2 antibody [EPR11242] (AB167399), expandable thumbnail](https://content.abcam.com/products/images/dync1i2-antibody-epr11242-ab167399--immunoprecipitation-img85555.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-DYNC1I2 antibody [EPR11242] (AB167399)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNC1I2 antibody [EPR11242] (AB167399), expandable thumbnail](https://content.abcam.com/products/images/dync1i2-antibody-epr11242-ab167399--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134850.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNC1I2 antibody [EPR11242] (AB167399)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Tested | Not recommended |
| Mouse | Predicted | Predicted | Not recommended | Predicted | Not recommended |
| Rat | Predicted | Predicted | Not recommended | Predicted | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function (PubMed:31079899). Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules (PubMed:31079899). The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCTN1 (By similarity). Involved in membrane-transport, such as Golgi apparatus, late endosomes and lysosomes (By similarity).
Alternative names
DNCI2, DNCIC2, DYNC1I2, Cytoplasmic dynein 1 intermediate chain 2, Cytoplasmic dynein intermediate chain 2, DH IC-2
Recommended products
Rabbit Recombinant Monoclonal DYNC1I2 antibody. Suitable for IHC-P, IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR11242
- Purity
- Tissue culture supernatant
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-DYNC1I2 antibody [EPR11242] (ab167399), expandable thumbnail](https://content.abcam.com/products/images/dync1i2-antibody-epr11242-ab167399--western-blot-img99372.jpg "Western blot - Anti-DYNC1I2 antibody [EPR11242] (ab167399)")
Western blot - Anti-DYNC1I2 antibody [EPR11242] (ab167399)
All lanes: Western blot - Anti-DYNC1I2 antibody [EPR11242] (ab167399) at 1/1000 dilution
Lane 1: Human fetal brain tissue lysate at 10 µg
Lane 2: SH-SY5Y cell lysate at 10 µg
Lane 3: HeLa cell lysate at 10 µg
Lane 4: 293T cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP conjugated at 1/2000 dilution
Predicted band size: 71 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNC1I2 antibody [EPR11242] (ab167399), expandable thumbnail](https://content.abcam.com/products/images/dync1i2-antibody-epr11242-ab167399--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42633.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNC1I2 antibody [EPR11242] (ab167399)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNC1I2 antibody [EPR11242] (ab167399)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling DYNC1I2 with ab167399 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-DYNC1I2 antibody [EPR11242] (ab167399), expandable thumbnail](https://content.abcam.com/products/images/dync1i2-antibody-epr11242-ab167399--immunoprecipitation-img85555.jpg "Immunoprecipitation - Anti-DYNC1I2 antibody [EPR11242] (ab167399)")
Immunoprecipitation - Anti-DYNC1I2 antibody [EPR11242] (ab167399)
Detection of DYNC1I2 by Western Blot of Immunprecipitate.
293T cell lysate immunoprecipitated using ab167399 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.All lanes: Immunoprecipitation - Anti-DYNC1I2 antibody [EPR11242] (ab167399)
Predicted band size: 71 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNC1I2 antibody [EPR11242] (ab167399), expandable thumbnail](https://content.abcam.com/products/images/dync1i2-antibody-epr11242-ab167399--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134850.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNC1I2 antibody [EPR11242] (ab167399)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNC1I2 antibody [EPR11242] (ab167399)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling DYNC1I2 with ab167399 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Product protocols
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