AB315245

Anti-Dynein antibody [EPR28484-147] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Dynein antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat, Recombinant full length protein - Human samples.

View Alternative Names

DLC2, DYNLL2, 8 kDa dynein light chain b, Dynein light chain LC8-type 2, DLC8b

4 Images

Supplier Data

Western blot - Anti-Dynein antibody [EPR28484-147] - BSA and Azide free (AB315245)

This data was developed using ab315244, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Huh7.

The lane 4 was developed using a high sensitivity ECL substrate.

The identity of the band at 75 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 59 seconds, lane 4 : 180 seconds

All lanes:

Western blot - Anti-Dynein antibody [EPR28484-147] (<a href='/en-us/products/primary-antibodies/dynein-antibody-epr28484-147-ab315244'>ab315244</a>) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Lane 4:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 10 kDa,36 kDa

false

Supplier Data

Western blot - Anti-Dynein antibody [EPR28484-147] - BSA and Azide free (AB315245)

This data was developed using ab315244, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen (PMID : 22891272).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Dynein antibody [EPR28484-147] (<a href='/en-us/products/primary-antibodies/dynein-antibody-epr28484-147-ab315244'>ab315244</a>) at 1/1000 dilution

Lane 1:

Mouse pancreas tissue lysate at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 10 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Dynein antibody [EPR28484-147] - BSA and Azide free (AB315245)

This data was developed using ab315244, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen (PMID : 22891272).

The identity of the band at 75 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Dynein antibody [EPR28484-147] (<a href='/en-us/products/primary-antibodies/dynein-antibody-epr28484-147-ab315244'>ab315244</a>) at 1/1000 dilution

Lane 1:

Rat testis tissue lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 10 kDa,36 kDa

false

Exposure time: 180s

Supplier Data

Western blot - Anti-Dynein antibody [EPR28484-147] - BSA and Azide free (AB315245)

This data was developed using ab315244, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human DYL1.

All lanes:

Western blot - Anti-Dynein antibody [EPR28484-147] (<a href='/en-us/products/primary-antibodies/dynein-antibody-epr28484-147-ab315244'>ab315244</a>) at 1/1000 dilution

Lane 1:

Human DYL2 recombinant protein at 10 ng

Lane 2:

Human DYL1 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 10 kDa

false

Exposure time: 180s

Unconjugated

Anti-Dynein antibody [EPR28484-147]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28484-147

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

ab315245 is the carrier-free version of ab315244.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The dynein protein a motor protein also known as cytoplasmic dynein plays an important role in cellular mechanics. It has a molecular mass of approximately 1.2 megadaltons. Dynein functions by converting chemical energy from ATP hydrolysis into mechanical work enabling movement along microtubules toward the minus end. Expressed widely in eukaryotic cells dynein is particularly found in the cytoplasm where it aids intracellular transport processes.

Biological function summary

Dynein facilitates important cellular processes by transporting organelles vesicles and even chromosomes. It is a major component of the dynactin complex a multi-subunit assembly that enhances dynein's affinity for microtubules and cargo. Through its interactions dynein maintains cellular organization and is pivotal in mitosis where it aligns chromosomes and pulls them apart during anaphase.

Pathways

The functionality of dynein interlinks with significant biological pathways such as the intracellular transport pathway and mitotic spindle formation. Partnering with proteins like kinesin dynein ensures the bidirectional transport necessary for cellular homeostasis. Additionally its role in spindle dynamics highlights interaction with spindle assembly checkpoint proteins orchestrating precise chromosome segregation.

Mutations or malfunctions in dynein can lead to neurodegenerative diseases such as amyotrophic lateral sclerosis (ALS) and spinal muscular atrophy (SMA). These disorders arise from defective axonal transport where dynein's role becomes disrupted. In the context of ALS dynein's interaction with superoxide dismutase 1 (SOD1) demonstrates its involvement in neuronal health and pathology. Similarly dynein dysfunction is linked to SMA where alterations affect neuromuscular communication and maintenance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in changing or maintaining the spatial distribution of cytoskeletal structures (By similarity).

See full target information DYNLL2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dynein antibody [EPR28484-147] - BSA and Azide free

Western blot - Anti-Dynein antibody [EPR28484-147] - BSA and Azide free (AB315245)

Western blot - Anti-Dynein antibody [EPR28484-147] - BSA and Azide free (AB315245)

Western blot - Anti-Dynein antibody [EPR28484-147] - BSA and Azide free (AB315245)

Western blot - Anti-Dynein antibody [EPR28484-147] - BSA and Azide free (AB315245)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise