Rabbit Recombinant Monoclonal DC1I1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-Dynein axonemal intermediate chain antibody [EPR11222-78] - BSA and Azide free (AB249505), expandable thumbnail](https://content.abcam.com/products/images/dynein-axonemal-intermediate-chain-antibody-epr11222-78-bsa-and-azide-free-ab249505--western-blot-img57448.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Dynein axonemal intermediate chain antibody [EPR11222-78] - BSA and Azide free (AB249505)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Predicted | Not recommended |
| Rat | Not recommended | Predicted | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Version
Target data
Function
Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. The intermediate chains mediate the binding of dynein to dynactin via its 150 kDa component (p150-glued) DCTN1. May play a role in mediating the interaction of cytoplasmic dynein with membranous organelles and kinetochores.
Alternative names
Cytoplasmic dynein 1 intermediate chain 1, Cytoplasmic dynein intermediate chain 1, DH IC-1, DNCIC1, DYNC1I1, DNCI1
Recommended products
Rabbit Recombinant Monoclonal DC1I1 antibody. Carrier free. Suitable for WB and reacts with Human samples.
Alternative names
Cytoplasmic dynein 1 intermediate chain 1, Cytoplasmic dynein intermediate chain 1, DH IC-1, DNCIC1, DYNC1I1, DNCI1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR11222-78
- Purification technique
Affinity purification
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab249505 is the carrier-free version of Anti-Dynein axonemal intermediate chain antibody [EPR11222-78] ab169758.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- Activity summary
Dynein axonemal intermediate chain sometimes referred to as IC or axonemal dynein plays an important role in cellular motility. This protein functions mechanically as a part of the dynein motor complex facilitating the sliding of microtubules that drive the movement of cilia and flagella. Dynein axonemal intermediate chain has a molecular mass ranging between 70 to 90 kDa making it an essential structural component of the axoneme in eukaryotic cells. This protein is ubiquitously expressed in cells possessing motile cilia and flagella including those in the respiratory tract and reproductive system.
- Biological function summary
Dynein axonemal intermediate chain is a critical part of the axonemal dynein complex where it bridges motor proteins to linkers ensuring the mechanical force required for ciliary and flagellar beating. It contributes to the motility of spermatozoa and the clearance of mucus in the respiratory epithelial cells. These activities rely on its interaction with heavy and light chains within the dynein motor complex forming a multi-subunit assembly that co-operatively performs directed movements.
- Pathways
Dynein axonemal intermediate chain integrates into cellular signaling pathways that control ciliary beating pattern an important pathway being the intraflagellar transport (IFT) system. This system coordinates with other proteins such as kinesin to regulate the bidirectional transport of ciliary components. The interplay between dynein complexes and IFT is essential for maintaining ciliary function and structure highlighting its pivotal role in motility-associated pathways.
- Associated diseases and disorders
Defects in dynein axonemal intermediate chain can lead to conditions such as Primary Ciliary Dyskinesia (PCD) and Kartagener's syndrome which manifest as chronic respiratory infections and infertility due to impaired ciliary function. This protein's dysfunction affects associated dynein proteins disrupting the normal architecture and function of axonemal complexes which contributes to the symptoms observed in these disorders. Addressing defects in this protein and its interactions holds potential for therapeutic advancements in treating ciliary dyskinesia-related ailments.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-Dynein axonemal intermediate chain antibody [EPR11222-78] - BSA and Azide free (ab249505), expandable thumbnail](https://content.abcam.com/products/images/dynein-axonemal-intermediate-chain-antibody-epr11222-78-bsa-and-azide-free-ab249505--western-blot-img57448.jpg "Western blot - Anti-Dynein axonemal intermediate chain antibody [EPR11222-78] - BSA and Azide free (ab249505)")
Western blot - Anti-Dynein axonemal intermediate chain antibody [EPR11222-78] - BSA and Azide free (ab249505)
This data was developed using Anti-Dynein axonemal intermediate chain antibody [EPR11222-78] ab169758, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Dynein axonemal intermediate chain antibody [EPR11222-78] (Anti-Dynein axonemal intermediate chain antibody [EPR11222-78] ab169758) at 1/1000 dilution
Lane 1: Human Fetal brain lysate at 10 µg
Lane 2: Human hypothalamus lysate at 10 µg
Predicted band size: 73 kDa
Downloads
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