AB249262

Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Dynein intermediate chain 1/DNAI1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Dynein axonemal intermediate chain 1, Axonemal dynein intermediate chain 1, DNAI1

2 Images

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Immunoprecipitation - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free (AB249262)

This data was developed using ab156567, the same antibody clone in a different buffer formulation.

Immunoprecipitation. ab156567 at 1/1000 staining Dynein intermediate chain 1/DNAI1 in human fetal brain lysate immunoprecipitated using ab156567 at 1/10. Predicted band size : 74 kDa.

All lanes:

Immunoprecipitation - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] (<a href='/en-us/products/primary-antibodies/dynein-intermediate-chain-1-dnai1-antibody-epr11222-ab156567'>ab156567</a>)

Predicted band size: 79 kDa

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Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free (AB249262)

This data was developed using ab156567, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] (<a href='/en-us/products/primary-antibodies/dynein-intermediate-chain-1-dnai1-antibody-epr11222-ab156567'>ab156567</a>) at 1/1000 dilution

Lane 1:

Fetal brain lysate at 10 µg

Lane 2:

Human hypothalamus lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 79 kDa

Observed band size: 74 kDa

false

Unconjugated

Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11222

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab249262 is the carrier-free version of ab156567.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dynein intermediate chain 1 also known as DNAI1 is a component of the cytoplasmic dynein complex. Mechanically this protein serves as an adaptor or linker within the larger dynein motor protein complex essential for intracellular transport. Its molecular mass is approximately 74 kDa. DNAI1 is expressed in a variety of tissues with higher expression observed in tissues involved in ciliary function. This protein is important for mediating cargo transport along microtubules contributing to cellular functions such as mitosis endocytosis and organelle positioning.

Biological function summary

Dynein intermediate chain 1 participates in key cellular processes linked to the movement of cilia and flagella. DNAI1 is an integral part of the axonemal dynein complex found in motile cilia. This complex enables the conversion of chemical energy from ATP into mechanical work driving the motion of these hair-like structures. Efficient cilia function relies on the coordinated action of axonemal dyneins enabling the clearance of mucus in respiratory pathways and movement of sperm in reproductive tracts.

Pathways

DNAI1 plays a role in the intraflagellar transport pathway important for building and maintaining cilia. Additionally DNAI1 is involved in the respiratory electron transport chain through its contribution to ciliary movement. The protein interacts closely with dynein heavy chain and dynein light chain proteins within these pathways ensuring the effective transport and movement of cellular components.

Defects in dynein intermediate chain 1 are linked to primary ciliary dyskinesia a genetic disorder affecting ciliary function. This disorder results in chronic respiratory tract infections and reduced fertility due to impaired ciliary motion. DNAI1 mutations can also connect to other ciliary dysfunctions impacting proteins like DNAH5 and DNAH11 which work alongside DNAI1 in the motility of cilia and flagella.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Part of the dynein complex of respiratory cilia.

See full target information DNAI1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell science 134: PubMed33310909

2021

Matrix stiffness regulates α-TAT1-mediated acetylation of α-tubulin and promotes silica-induced epithelial-mesenchymal transition via DNA damage.

Applications

Unspecified application

Species

Unspecified reactive species

Gengxu Li,Si Chen,Yi Zhang,Hong Xu,Dingjie Xu,Zhongqiu Wei,Xuemin Gao,Wenchen Cai,Na Mao,Lijuan Zhang,Shumin Li,Fang Yang,Heliang Liu,Shifeng Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free

Immunoprecipitation - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free (AB249262)

Western blot - Anti-Dynein intermediate chain 1/DNAI1 antibody [EPR11222] - BSA and Azide free (AB249262)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise