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AB236594

Anti-Dynein light chain antibody

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(1 Publication)

Rabbit Polyclonal Dynein light chain antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DNAL4.

View Alternative Names

Dynein axonemal light chain 4, DNAL4

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynein light chain antibody (AB236594)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynein light chain antibody (AB236594)

Paraffin-embedded human testis tissue stained for Dynein light chain using ab236594 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynein light chain antibody (AB236594)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dynein light chain antibody (AB236594)

Paraffin-embedded human breast cancer tissue stained for Dynein light chain using ab236594 at 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-Dynein light chain antibody (AB236594)
  • WB

Supplier Data

Western blot - Anti-Dynein light chain antibody (AB236594)

All lanes:

Western blot - Anti-Dynein light chain antibody (ab236594) at 1/500 dilution

Lane 1:

Mouse gonadal lysate

Lane 2:

HL-60 (human promyelocytic leukemia cell line) whole cell lysate

Lane 3:

NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate

Lane 4:

Mouse brain lysate

Secondary

All lanes:

Goat polyclonal to Rabbit IgG at 1/10000 dilution

Predicted band size: 12 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-Dynein light chain antibody (AB236594)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dynein light chain antibody (AB236594)

MCF7 (human breast adenocarcinoma cell line) cells stained for Dynein light chain (green) using ab236594 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human DNAL4.

O96015

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dynein light chain also known as DLC is a component of the dynein protein complex. This complex functions as a motor protein driving cellular transport by walking along microtubules using ATP hydrolysis. Dynein light chain is small approximately 8 kDa in mass yet it plays an integral role in dynein's mechanical operation. These proteins are ubiquitously expressed throughout various tissues in eukaryotic organisms including humans where they assist in the intracellular transport of organelles proteins and RNA.
Biological function summary

Dynein light chain is essential for the function and stability of the dynein motor complex which also includes heavy chains intermediate chains and other light chains. The dynein complex mediates the retrograde transport along axons and is involved in cell division by contributing to spindle positioning. Dynein light chain binds to cargo adaptor proteins and helps the dynein complex anchor to various cellular structures ensuring targeted transport within the cell.

Pathways

The dynein light chain plays an important role in the intracellular transport pathway particularly in vesicle trafficking and organelle positioning. It interacts with key proteins such as dynactin an important cofactor that enhances dynein's motor function and cargo binding. Additionally dynein light chain participates in mitotic spindle orientation collaborating with proteins like LIS1 to ensure proper chromosome segregation during mitosis.

Dysregulation of dynein light chain function is linked to neurodegenerative conditions such as Huntington's disease and motor neuron disorders. Alterations in dynein-mediated transport can lead to the accumulation of protein aggregates and impaired neural function. In Huntington's disease this disruption affects the dynein and dynactin interaction contributing to neuronal dysfunction and cell death. Dynein light chain's efficiency and integrity also intersect with LIS1 in lissencephaly where reduced dynein activity can result in abnormal neuronal migration and brain structure abnormalities.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity (By similarity).
See full target information DNAL4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106603 PubMed37128611

2023

Gαs slow conformational transition upon GTP binding and a novel Gαs regulator.

Applications

Unspecified application

Species

Unspecified reactive species

Donghoon Ahn,Davide Provasi,Nguyen Minh Duc,Jun Xu,Leslie Salas-Estrada,Aleksandar Spasic,Min Woo Yun,Juyeong Kang,Dongmin Gim,Jaecheol Lee,Yang Du,Marta Filizola,Ka Young Chung
View all publications
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Alternative Products

Primary Antibodies

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Secondary Antibodies

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Primary Antibodies

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Primary Antibodies

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