AB249474

Anti-Dynein light chain antibody [EPR11234] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal Dynein light chain antibody. Carrier free. Suitable for IP, WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Dynein axonemal light chain 4, DNAL4

2 Images

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Immunoprecipitation - Anti-Dynein light chain antibody [EPR11234] - BSA and Azide free (AB249474)

This data was developed using ab168385, the same antibody clone in a different buffer formulation.Detection of Dynein light chain by Western Blot of Immunprecipitate. Mouse testis lysate immunoprecipitated using ab168385 at 1/10 dilution; HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

All lanes:

Immunoprecipitation - Anti-Dynein light chain antibody [EPR11234] (<a href='/en-us/products/primary-antibodies/dynein-light-chain-antibody-epr11234-ab168385'>ab168385</a>)

Predicted band size: 12 kDa

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Western blot - Anti-Dynein light chain antibody [EPR11234] - BSA and Azide free (AB249474)

This data was developed using ab168385, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Dynein light chain antibody [EPR11234] (<a href='/en-us/products/primary-antibodies/dynein-light-chain-antibody-epr11234-ab168385'>ab168385</a>) at 1/1000 dilution

All lanes:

Mouse testis lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 12 kDa

false

Unconjugated

Anti-Dynein light chain antibody [EPR11234]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11234

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

ab249474 is the carrier-free version of ab168385.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dynein light chain also known as DLC is a component of the dynein protein complex. This complex functions as a motor protein driving cellular transport by walking along microtubules using ATP hydrolysis. Dynein light chain is small approximately 8 kDa in mass yet it plays an integral role in dynein’s mechanical operation. These proteins are ubiquitously expressed throughout various tissues in eukaryotic organisms including humans where they assist in the intracellular transport of organelles proteins and RNA.

Biological function summary

Dynein light chain is essential for the function and stability of the dynein motor complex which also includes heavy chains intermediate chains and other light chains. The dynein complex mediates the retrograde transport along axons and is involved in cell division by contributing to spindle positioning. Dynein light chain binds to cargo adaptor proteins and helps the dynein complex anchor to various cellular structures ensuring targeted transport within the cell.

Pathways

The dynein light chain plays an important role in the intracellular transport pathway particularly in vesicle trafficking and organelle positioning. It interacts with key proteins such as dynactin an important cofactor that enhances dynein's motor function and cargo binding. Additionally dynein light chain participates in mitotic spindle orientation collaborating with proteins like LIS1 to ensure proper chromosome segregation during mitosis.

Dysregulation of dynein light chain function is linked to neurodegenerative conditions such as Huntington's disease and motor neuron disorders. Alterations in dynein-mediated transport can lead to the accumulation of protein aggregates and impaired neural function. In Huntington’s disease this disruption affects the dynein and dynactin interaction contributing to neuronal dysfunction and cell death. Dynein light chain’s efficiency and integrity also intersect with LIS1 in lissencephaly where reduced dynein activity can result in abnormal neuronal migration and brain structure abnormalities.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Force generating protein of respiratory cilia. Produces force towards the minus ends of microtubules. Dynein has ATPase activity (By similarity).

See full target information DNAL4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dynein light chain antibody [EPR11234] - BSA and Azide free

Immunoprecipitation - Anti-Dynein light chain antibody [EPR11234] - BSA and Azide free (AB249474)

Western blot - Anti-Dynein light chain antibody [EPR11234] - BSA and Azide free (AB249474)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Product promise