Anti-DYNLL1/PIN antibody [EP1660Y] - BSA and Azide free

• ICC/IF

AbReview16748****

Immunocytochemistry/ Immunofluorescence - Anti-DYNLL1/PIN antibody [EP1660Y] - BSA and Azide free (AB232343)

Unpurified ab51603 staining DLC8 in mouse kidney cells cells by ICC/IF (immunocytochemistry/immunofluorescence. Cells were fixed with methanol, permeabilized with 0.1% Triton and blocked with 1% milk for 1 hour at room temperature. The sample was incubated with primary antibody (1/400; 1% milk in PBS) for 16 hours at 4°C. An Alexa Fluor^®488-conjugated Goat polyclonal to rabbit IgG (1/1000) was used as secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51603).

This image is courtesy of an anonymous Abreview.

• IP

PubMed

Immunoprecipitation - Anti-DYNLL1/PIN antibody [EP1660Y] - BSA and Azide free (AB232343)

Unpurified ab51603 used in IP.SKAP and Astrin form a complex. (A, left) Silver-stained gels showing a one-step IP of GFPLAP-Astrin, GFPLAP-SKAP, or GFPLAP-LC8. (A, right) Data from the mass spectrometric analysis of the purifications indicating the percent sequence coverage from each IP. (B) Silver-stained gel showing the purification of FLAG-SKAP from chicken DT40 cells relative to controls. The indicated proteins were identified by excising them from a gel and analyzing them by mass spectrometry.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51603).

All lanes:

Immunoprecipitation - Anti-DYNLL1/PIN antibody [EP1660Y] (<a href='/en-us/products/primary-antibodies/dynll1-pin-antibody-ep1660y-ab51603'>ab51603</a>)

Predicted band size: 10 kDa

false

Image from Schmidt JC et al. J Cell Biol. 2010 Oct 18;191(2):269-80. Epub 2010 Oct 11 Fig 2. DOI 10.1083/jcb.201006129.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNLL1/PIN antibody [EP1660Y] - BSA and Azide free (AB232343)

Immunohistochemical staining of paraffin embedded human liver using unpurified ab51603 (1/100).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51603).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYNLL1/PIN antibody [EP1660Y] - BSA and Azide free (AB232343)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling DYNLL1 with Purified ab51603 at 1 : 500 dilution (1.34 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1 : 0 dilution. PBS instead of the primary antibody was used as the negative control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51603).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DYNLL1/PIN antibody [EP1660Y] - BSA and Azide free (AB232343)

Immunocytochemistry/ Immunofluorescence analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling DYNLL1 with Purified ab51603 at 1 : 100 dilution (6.7μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor^® 488) was used as the secondary antibody at 1 : 1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51603).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-DYNLL1/PIN antibody [EP1660Y] - BSA and Azide free (AB232343)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling DYNLL1 / PIN (red) with purified ab51603 at a 1/2300 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51603).

• IP

Unknown

Immunoprecipitation - Anti-DYNLL1/PIN antibody [EP1660Y] - BSA and Azide free (AB232343)

ab51603 (purified) at 1 : 30 dilution (2ug) immunoprecipitating DYNLL1 / PIN in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate.

Lane 1 (input) : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10ug
Lane 2 (+) : ab51603 & HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab51603 in HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate

For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1 : 1000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51603).

All lanes:

Immunoprecipitation - Anti-DYNLL1/PIN antibody [EP1660Y] (<a href='/en-us/products/primary-antibodies/dynll1-pin-antibody-ep1660y-ab51603'>ab51603</a>)

Predicted band size: 10 kDa

false