Anti-DYNLT3 antibody

• WB

Supplier Data

Western blot - Anti-DYNLT3 antibody (AB121209)

All lanes:

Western blot - Anti-DYNLT3 antibody (ab121209)

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

NBT-II (rat urinary bladder tumor cell line) whole cell lysate

Predicted band size: 13 kDa

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• WB

Supplier Data

Western blot - Anti-DYNLT3 antibody (AB121209)

Loading control : Anti-PFN1.

All lanes:

Western blot - Anti-DYNLT3 antibody (ab121209)

Lane 1:

SK-MEL-30 (human melanoma cell line) whole cell lysate

Lane 2:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate

Predicted band size: 13 kDa

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• WB

Lab

Western blot - Anti-DYNLT3 antibody (AB121209)

False colour image of Western blot : Anti-DYNLT3 antibody (ab121209) staining at 0.04 μg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red.

In Western blot, ab121209 was shown to bind specifically to DYNLT3. A band was observed at 13 kDa in wild-type HeLa cell lysates with no signal observed at this size in DYNLT3 CRISPR-Cas9 edited cell lysates. The band observed in the CRISPR-Cas9 edited lysate lane above 13 kDa is likely to represent DYNLT3 with an insertion. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and DYNLT3 CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^® (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-DYNLT3 antibody (ab121209) at 0.04 µg/mL

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

DYNLT3 knockout HeLa cell lysate at 20 µg

Lane 3:

SH-SY5Y cell lysate at 20 µg

Lane 4:

U937 cell lysate at 20 µg

Predicted band size: 13 kDa

Observed band size: 13 kDa

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