AB113968

Anti-Dyrk1B/MIRK antibody

Name: Anti-Dyrk1B/MIRK antibody (ab113968) | Abcam
Brand: Abcam Limited
SKU: AB113968

(1 Review)

(1 Publication)

Rabbit Polyclonal Dyrk1B/MIRK antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human DYRK1B aa 500-550.

View Alternative Names

MIRK, DYRK1B, Dual specificity tyrosine-phosphorylation-regulated kinase 1B, Minibrain-related kinase, Mirk protein kinase

2 Images

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Immunoprecipitation - Anti-Dyrk1B/MIRK antibody (AB113968)

Detection of Dyrk1B/MIRK in Immunoprecipitates of HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab113968 at 10 μg/mg lysate for IP (Lane 1) and at 1 μg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-Dyrk1B/MIRK antibody (ab113968)

Predicted band size: 69 kDa

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Unknown

Western blot - Anti-Dyrk1B/MIRK antibody (AB113968)

All lanes:

Western blot - Anti-Dyrk1B/MIRK antibody (ab113968) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 69 kDa

true

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DYRK1B aa 500-550. The exact immunogen used to generate this antibody is proprietary information.

Q9Y463

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5-15 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Bat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dyrk1B also referred to as Mirk is a protein kinase with a molecular mass of approximately 65 kDa. It belongs to the DYRK family which shares characteristics with dual-specificity kinases. Dyrk1B plays a role in phosphorylation activities which involves the addition of phosphate groups to target proteins altering their activity. This kinase is expressed in various tissues notably in muscle and certain cancer cells where it influences cellular processes such as proliferation and survival.

Biological function summary

Dyrk1B affects cell cycle regulation and differentiation. It is involved in promoting cell cycle exit and muscle differentiation by phosphorylating specific transcription factors. Dyrk1B exists as a monomer not requiring interaction with other proteins to function. By regulating transcription factors it contributes to the control of growth-restrictive environments and supports cell survival under stress conditions.

Pathways

Dyrk1B interacts with multiple signaling pathways including the Ras/MAPK and PI3K/Akt pathways. In these pathways Dyrk1B interacts with proteins such as p27Kip1 and FOXO impacting cellular quiescence and survival. Its influence in these pathways suggests a role in controlling important mechanism of cell cycle arrest and resistance to stress.

Dyrk1B has relevance to certain cancers and metabolic disorders like type 2 diabetes. In pancreatic cancer Dyrk1B supports cell survival and resistance to chemotherapy. Additionally its dysregulation impacts insulin resistance in metabolic disorders. Cancers often show upregulation of Dyrk1B working in conjunction with kinases like AKT to enhance tumor survival and progression providing potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Dual-specificity kinase which possesses both serine/threonine and tyrosine kinase activities. Plays an essential role in ribosomal DNA (rDNA) double-strand break repair and rDNA copy number maintenance (PubMed : 33469661). During DNA damage, mediates transcription silencing in part via phosphorylating and enforcing DSB accumulation of the histone methyltransferase EHMT2 (PubMed : 32611815). Enhances the transcriptional activity of TCF1/HNF1A and FOXO1. Inhibits epithelial cell migration. Mediates colon carcinoma cell survival in mitogen-poor environments. Inhibits the SHH and WNT1 pathways, thereby enhancing adipogenesis. In addition, promotes expression of the gluconeogenic enzyme glucose-6-phosphatase catalytic subunit 1 (G6PC1).

See full target information DYRK1B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 7:46085 PubMed28443635

2017

Expression and localisation of two-pore domain (K2P) background leak potassium ion channels in the mouse retina.

Applications

Unspecified application

Species

Unspecified reactive species

Steven Hughes,Russell G Foster,Stuart N Peirson,Mark W Hankins

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Dyrk1B/MIRK antibody

Immunoprecipitation - Anti-Dyrk1B/MIRK antibody (AB113968)

Western blot - Anti-Dyrk1B/MIRK antibody (AB113968)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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