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AB133652

Anti-Dysbindin antibody [EPR7041]

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(5 Publications)

Rabbit Recombinant Monoclonal Dysbindin antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 5 publications.

View Alternative Names

BLOC1S8, My031, DTNBP1, Dysbindin, Biogenesis of lysosome-related organelles complex 1 subunit 8, Dysbindin-1, Dystrobrevin-binding protein 1, Hermansky-Pudlak syndrome 7 protein, BLOC-1 subunit 8, HPS7 protein

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysbindin antibody [EPR7041] (AB133652)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysbindin antibody [EPR7041] (AB133652)

Immunohistochemical analysis of Dysbindin in paraffin embedded Human kidney tissue labelled with ab133652 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-Dysbindin antibody [EPR7041] (AB133652)
  • WB

Unknown

Western blot - Anti-Dysbindin antibody [EPR7041] (AB133652)

All lanes:

Western blot - Anti-Dysbindin antibody [EPR7041] (ab133652) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 2:

HeLa cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

Human fetal lung tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 39 kDa

false

OI-RD Scanning - Anti-Dysbindin antibody [EPR7041] (AB133652)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-Dysbindin antibody [EPR7041] (AB133652)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

  • Carrier free

    Anti-Dysbindin antibody [EPR7041] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7041

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dysbindin also known as Dystrobrevin Binding Protein 1 (DTNBP1) is an important protein that plays an important role in cellular processes. Its molecular weight is approximately 40 kDa. Dysbindin is expressed in a variety of tissues but it shows higher expression in the brain and testis. Within cells dysbindin localizes to the cytoplasm and is notably present at synaptic sites where it assumes an important function in neuronal communication.
Biological function summary

Multiple cellular processes involve dysbindin including synaptic transmission and intracellular signaling. Dysbindin is a component of the Biogenesis of Lysosome-related Organelles Complex 1 (BLOC-1) which is important for the biogenesis and trafficking of lysosome-related organelles. Its role in ensuring proper lysosomal function is linked to the regulation of synaptic vesicle release influencing neurotransmitter availability in synaptic clefts.

Pathways

Several important biological networks engage dysbindin particularly affecting neural and cellular functions. It participates in the synaptic vesicle cycle and pigment cell pathways. Dysbindin’s functional relationship with proteins such as Snapin and Pallidin illustrates its connection to BLOC-1-related pathways indicating its integrative role in synaptic endosome trafficking and pigment gene expression processes.

Dysbindin has a significant association with schizophrenia and Hermansky-Pudlak syndrome type 7. Genetic studies have linked the dysbindin gene to altered risk for schizophrenia through its impact on synaptic function and plasticity. Additionally its interaction with other BLOC-1 components like Pallidin ties it to Hermansky-Pudlak syndrome where disrupted organelle trafficking affects pigmentation and leads to systemic symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Associates with the BLOC-2 complex to facilitate the transport of TYRP1 independent of AP-3 function. Plays a role in synaptic vesicle trafficking and in neurotransmitter release. Plays a role in the regulation of cell surface exposure of DRD2. May play a role in actin cytoskeleton reorganization and neurite outgrowth. May modulate MAPK8 phosphorylation. Appears to promote neuronal transmission and viability through regulating the expression of SNAP25 and SYN1, modulating PI3-kinase-Akt signaling and influencing glutamatergic release. Regulates the expression of SYN1 through binding to its promoter. Modulates prefrontal cortical activity via the dopamine/D2 pathway.
See full target information DTNBP1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Journal of neural transmission (Vienna, Austria : 1996) 128:701-709 PubMed33890175

2021

Cortical copper transporter expression in schizophrenia: interactions of risk gene dysbindin-1.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsten E Schoonover,William M Kennedy,Rosalinda C Roberts

Schizophrenia research 228:60-73 PubMed33434736

2021

Abnormalities in the copper transporter CTR1 in postmortem hippocampus in schizophrenia: A subregion and laminar analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsten E Schoonover,Charlene B Farmer,Charity J Morgan,Vidushi Sinha,Laura Odom,Rosalinda C Roberts

Schizophrenia research 228:124-133 PubMed33434726

2021

Markers of copper transport in the cingulum bundle in schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsten E Schoonover,Rosalinda C Roberts

The world journal of biological psychiatry : the official journal of the World Federation of Societies of Biological Psychiatry 21:13-28 PubMed30230404

2018

Impaired copper transport in schizophrenia results in a copper-deficient brain state: A new side to the dysbindin story.

Applications

Unspecified application

Species

Unspecified reactive species

Kirsten E Schoonover,Stacy L Queern,Suzanne E Lapi,Rosalinda C Roberts

Journal of neurodevelopmental disorders 8:14 PubMed27134685

2016

Effects of sex and DTNBP1 (dysbindin) null gene mutation on the developmental GluN2B-GluN2A switch in the mouse cortex and hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Duncan Sinclair,Joseph Cesare,Mary McMullen,Greg C Carlson,Chang-Gyu Hahn,Karin E Borgmann-Winter
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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