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AB327131

Anti-DYSF Antibody [CL10888]

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Mouse Monoclonal Dysferlin antibody. Suitable for WB, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human DYSF.

View Alternative Names

FER1L1, DYSF, Dysferlin, Dystrophy-associated fer-1-like protein, Fer-1-like protein 1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYSF Antibody [CL10888] (AB327131)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYSF Antibody [CL10888] (AB327131)

Immunohistochemical staining of human lymph node using ab327131 at 1 : 1000–1 : 2500 dilution, showing strong membranous positivity in non-germinal center cells and endothelial cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYSF Antibody [CL10888] (AB327131)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYSF Antibody [CL10888] (AB327131)

Immunohistochemistry analysis in human spleen and skin tissues using ab327131 at 1 : 1000–1 : 2500 dilution. Corresponding DYSF RNA-seq data are presented for the same tissues.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYSF Antibody [CL10888] (AB327131)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYSF Antibody [CL10888] (AB327131)

Immunohistochemical staining of human spleen using ab327131 at 1 : 1000–1 : 2500 dilution, showing moderate positivity in cells in red pulp.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYSF Antibody [CL10888] (AB327131)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYSF Antibody [CL10888] (AB327131)

Immunohistochemical staining of human placenta using ab327131 at 1 : 1000–1 : 2500 dilution, showing moderate membranous positivity in trophoblastic cells.

Western blot - Anti-DYSF Antibody [CL10888] (AB327131)
  • WB

Supplier Data

Western blot - Anti-DYSF Antibody [CL10888] (AB327131)

Western blot analysis in human cell line RH-30 using ab327131 at 1 µg/ml.

All lanes:

Western blot - Anti-DYSF Antibody [CL10888] (ab327131)

All lanes:

Human cell line RH-30

Predicted band size: 237.3 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYSF Antibody [CL10888] (AB327131)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DYSF Antibody [CL10888] (AB327131)

Immunohistochemical staining of human skin using ab327131 at 1 : 1000–1 : 2500 dilution, showing no positivity in keratinocytes as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL10888

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DYSF.

O75923

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity).
See full target information DYSF
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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