AB15108

Anti-Dysferlin antibody

(4 Reviews)

(5 Publications)

Rabbit Polyclonal Dysferlin antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 5 publications.

View Alternative Names

FER1L1, DYSF, Dysferlin, Dystrophy-associated fer-1-like protein, Fer-1-like protein 1

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysferlin antibody (AB15108)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysferlin antibody (AB15108)

ab15108 staining Dysferlin in human skeletal muscle by Immunohistochemistry (FFPE-sections).

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Western blot - Anti-Dysferlin antibody (AB15108)

All lanes:

Western blot - Anti-Dysferlin antibody (ab15108) at 1 µg/mL

All lanes:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 237 kDa

Observed band size: 231 kDa,240 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dysferlin also known by its alternate names DYSF and CAPN3 is a protein characterized by a mass of approximately 237 kilodaltons. It belongs to the ferlin family and is mainly expressed in skeletal and cardiac muscle tissues. This protein contains multiple C2 domains which are significant for its membrane-trafficking roles. Dysferlin has an important role at the muscle surface particularly in the repair of sarcolemma breaches which helps maintain muscle cell integrity.

Biological function summary

The action of dysferlin impacts several molecular processes critical for muscle function and maintenance often interacting as part of a larger protein complex. It facilitates membrane repair by promoting vesicle fusion events and recruits other proteins to damaged sites. Such action is vital in restoring cellular structure after membrane damage particularly under the mechanical stress experienced in muscle tissues.

Pathways

Dysferlin operates within the calcium-dependent membrane repair pathway and the broader muscular dystrophy pathway. It interacts importantly with proteins such as annexins and actin that aid in membrane repair processes. Dysferlin's role in cellular repair connects it to pathways related to muscle membrane dynamics and stress response further highlighting its function in muscle health and recovery.

Mutations or deficiencies in dysferlin are linked to muscular dystrophies such as limb-girdle muscular dystrophy type 2B and Miyoshi myopathy. These conditions are characterized by progressive muscle weakness and wasting. The link between dysferlin's function and these disorders is also seen in its association with proteins like calpain 3 which also plays a role in muscle maintenance. Understanding the line of interaction between dysferlin and these proteins offers insights into potential therapeutic targets for these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity).

See full target information DYSF

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:6849 PubMed36369230

2022

Differential impact of ubiquitous and muscle dynamin 2 isoforms in muscle physiology and centronuclear myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Raquel Gómez-Oca,Evelina Edelweiss,Sarah Djeddi,Mathias Gerbier,Xènia Massana-Muñoz,Mustapha Oulad-Abdelghani,Corinne Crucifix,Coralie Spiegelhalter,Nadia Messaddeq,Pierre Poussin-Courmontagne,Pascale Koebel,Belinda S Cowling,Jocelyn Laporte

Frontiers in neuroscience 9:16 PubMed25729345

2015

The inner CSF-brain barrier: developmentally controlled access to the brain via intercellular junctions.

Applications

IHC

Species

Unspecified reactive species

Sophie Whish,Katarzyna M Dziegielewska,Kjeld Møllgård,Natassya M Noor,Shane A Liddelow,Mark D Habgood,Samantha J Richardson,Norman R Saunders

Neuromuscular disorders : NMD 20:820-5 PubMed20817457

2010

Evaluation of commercial dysferlin antibodies on canine, mouse and human skeletal muscle.

Applications

WB, WB, WB

Species

Human, Dog, Mouse

Ling T Guo,Steven A Moore,Sonia Forcales,Eva Engvall,G Diane Shelton

Human molecular genetics 18:318-27 PubMed18986978

2008

Myogenic Akt signaling upregulates the utrophin-glycoprotein complex and promotes sarcolemma stability in muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Angela K Peter,Christopher Y Ko,Michelle H Kim,Nigel Hsu,Noriyuki Ouchi,Suhn Rhie,Yasuhiro Izumiya,Ling Zeng,Kenneth Walsh,Rachelle H Crosbie

FASEB journal : official publication of the Federa 21:1768-76 PubMed17363620

2007

From T-tubule to sarcolemma: damage-induced dysferlin translocation in early myogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lars Klinge,Steve Laval,Sharon Keers,Faye Haldane,Volker Straub,Rita Barresi,Kate Bushby

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Dysferlin antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysferlin antibody (AB15108)

Western blot - Anti-Dysferlin antibody (AB15108)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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