Rabbit Recombinant Monoclonal Dysferlin antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (AB209877), expandable thumbnail](https://content.abcam.com/products/images/dysferlin-antibody-jai-1-49-3-bsa-and-azide-free-ab209877--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41311.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (AB209877)")
![Immunocytochemistry/ Immunofluorescence - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (AB209877), expandable thumbnail](https://content.abcam.com/products/images/dysferlin-antibody-jai-1-49-3-bsa-and-azide-free-ab209877--immunocytochemistry-immunofluorescence-img70132.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (AB209877)")
![Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (AB209877), expandable thumbnail](https://content.abcam.com/products/images/dysferlin-antibody-jai-1-49-3-bsa-and-azide-free-ab209877--immunohistochemistry-frozen-sections-img9769.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (AB209877)")
![Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (AB209877), expandable thumbnail](https://content.abcam.com/products/images/dysferlin-antibody-jai-1-49-3-bsa-and-azide-free-ab209877--immunohistochemistry-frozen-sections-img97371.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (AB209877)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt | WB | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Tested | Tested |
| Mouse | Predicted | Not recommended | Expected | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
Key calcium ion sensor involved in the Ca(2+)-triggered synaptic vesicle-plasma membrane fusion. Plays a role in the sarcolemma repair mechanism of both skeletal muscle and cardiomyocytes that permits rapid resealing of membranes disrupted by mechanical stress (By similarity).
Alternative names
FER1L1, DYSF, Dysferlin, Dystrophy-associated fer-1-like protein, Fer-1-like protein 1
Recommended products
Rabbit Recombinant Monoclonal Dysferlin antibody. Carrier free. Suitable for IHC-P, WB, ICC/IF, IHC-Fr and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- JAI-1-49-3
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 7.2 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab209877 is the carrier-free version of Anti-Dysferlin antibody [JAI-1-49-3] ab124684.
This antibody was made in collaboration with the Jain Foundation whose goal is to hasten EVERY avenue that may lead to the cure for LGMD2B/Miyoshi.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Dysferlin also known by its alternate names DYSF and CAPN3 is a protein characterized by a mass of approximately 237 kilodaltons. It belongs to the ferlin family and is mainly expressed in skeletal and cardiac muscle tissues. This protein contains multiple C2 domains which are significant for its membrane-trafficking roles. Dysferlin has an important role at the muscle surface particularly in the repair of sarcolemma breaches which helps maintain muscle cell integrity.
- Biological function summary
The action of dysferlin impacts several molecular processes critical for muscle function and maintenance often interacting as part of a larger protein complex. It facilitates membrane repair by promoting vesicle fusion events and recruits other proteins to damaged sites. Such action is vital in restoring cellular structure after membrane damage particularly under the mechanical stress experienced in muscle tissues.
- Pathways
Dysferlin operates within the calcium-dependent membrane repair pathway and the broader muscular dystrophy pathway. It interacts importantly with proteins such as annexins and actin that aid in membrane repair processes. Dysferlin's role in cellular repair connects it to pathways related to muscle membrane dynamics and stress response further highlighting its function in muscle health and recovery.
- Associated diseases and disorders
Mutations or deficiencies in dysferlin are linked to muscular dystrophies such as limb-girdle muscular dystrophy type 2B and Miyoshi myopathy. These conditions are characterized by progressive muscle weakness and wasting. The link between dysferlin's function and these disorders is also seen in its association with proteins like calpain 3 which also plays a role in muscle maintenance. Understanding the line of interaction between dysferlin and these proteins offers insights into potential therapeutic targets for these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (ab209877), expandable thumbnail](https://content.abcam.com/products/images/dysferlin-antibody-jai-1-49-3-bsa-and-azide-free-ab209877--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41311.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (ab209877)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (ab209877)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling Dysferlin with purified Anti-Dysferlin antibody [JAI-1-49-3] ab124684 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dysferlin antibody [JAI-1-49-3] ab124684).
![Immunocytochemistry/ Immunofluorescence - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (ab209877), expandable thumbnail](https://content.abcam.com/products/images/dysferlin-antibody-jai-1-49-3-bsa-and-azide-free-ab209877--immunocytochemistry-immunofluorescence-img70132.jpg "Immunocytochemistry/ Immunofluorescence - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (ab209877)")
Immunocytochemistry/ Immunofluorescence - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (ab209877)
Immunocytochemistry/Immunofluorescence analysis of A673 cells labelling Dysferlin with purified Anti-Dysferlin antibody [JAI-1-49-3] ab124684 at 1/300. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: primary antibody (1/300) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dysferlin antibody [JAI-1-49-3] ab124684).
![Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (ab209877), expandable thumbnail](https://content.abcam.com/products/images/dysferlin-antibody-jai-1-49-3-bsa-and-azide-free-ab209877--immunohistochemistry-frozen-sections-img9769.jpg "Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (ab209877)")
Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (ab209877)
Immunohistochemistry (Frozen sections) analysis of unfixed frozen mouse skeletal muscle tissue (wild type) labelling Dysferlin with unpurified Anti-Dysferlin antibody [JAI-1-49-3] ab124684 at a dilution of 1/200.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dysferlin antibody [JAI-1-49-3] ab124684).
![Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (ab209877), expandable thumbnail](https://content.abcam.com/products/images/dysferlin-antibody-jai-1-49-3-bsa-and-azide-free-ab209877--immunohistochemistry-frozen-sections-img97371.jpg "Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (ab209877)")
Immunohistochemistry (Frozen sections) - Anti-Dysferlin antibody [JAI-1-49-3] - BSA and Azide free (ab209877)
Immunohistochemistry (Frozen sections) analysis of unfixed frozen human skeletal muscle tissue (control) labelling Dysferlin with unpurified Anti-Dysferlin antibody [JAI-1-49-3] ab124684 at a dilution of 1/200.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Dysferlin antibody [JAI-1-49-3] ab124684).
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