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AB234644

Anti-Dystonin/BPA antibody

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(1 Publication)

Rabbit Polyclonal Dystonin/BPA antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DST aa 1-200.

View Alternative Names

BP230, BP240, BPAG1, DMH, DT, KIAA0728, DST, Dystonin, 230 kDa bullous pemphigoid antigen, 230/240 kDa bullous pemphigoid antigen, Bullous pemphigoid antigen 1, Dystonia musculorum protein, Hemidesmosomal plaque protein, BPA, Bullous pemphigoid antigen

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Dystonin/BPA antibody (AB234644)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dystonin/BPA antibody (AB234644)

HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Dystonin/BPA (green) using ab234644 at 1/100 dilution in ICC/IF, followed by Alexa-Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).

Western blot - Anti-Dystonin/BPA antibody (AB234644)
  • WB

Supplier Data

Western blot - Anti-Dystonin/BPA antibody (AB234644)

All lanes:

Western blot - Anti-Dystonin/BPA antibody (ab234644) at 1/500 dilution

Lane 1:

Recombinant human Dystonin/BPA protein at 0.1 µg

Lane 2:

Recombinant human Dystonin/BPA protein at 0.05 µg

Lane 3:

Recombinant human Dystonin/BPA protein at 0.025 µg

Lane 4:

Recombinant human Dystonin/BPA protein at 0.0125 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 860 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human DST aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q03001

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purity >95%.
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dystonin also known as BPA (Bullous Pemphigoid Antigen) is a large protein with a molecular mass of approximately 800 kDa. This versatile protein plays a mechanical role by providing structural integrity to cells particularly within tissues that experience mechanical stress. Dystonin locates mainly in the cytoskeleton-associated regions of muscle and neural cells where it connects cytoskeletal components to membrane complexes. The presence of dystonin in these areas indicates its function in maintaining tissue cohesion and stability under stress.
Biological function summary

Dystonin acts as a cytoskeletal linker that maintains cellular architecture by directly linking intermediate filaments to the actin and microtubule networks. As part of this structural network dystonin forms complexes with other proteins to stabilize cell junctions and muscle fibers. In neural tissues it plays an important role in maintaining the integrity of axons essential for proper nerve function. Dystonin ensures cells retain their shape and positioning which is necessary for regular cellular functions and signal transmissions in these tissues.

Pathways

Dystonin interacts with multiple structural proteins in the cytoskeletal pathway such as keratins and microtubule-associated proteins. The linkage function of dystonin integrates it into the hemidesmosomal pathway which is vital for epithelial cell adhesion. Dystonin works along with other proteins like integrins and plectin which also engage in these pathways promoting cellular adhesion and resilience against mechanical stress.

Mutations or deficiencies in dystonin link to diseases like bullous pemphigoid and Hereditary Sensory and Autonomic Neuropathy Type VI (HSAN VI). Bullous pemphigoid is an autoimmune disorder characterized by blistering of the skin due to compromised cell adhesion. The lack of functional dystonin in HSAN VI leads to nerve degradation highlighting its role in neural maintenance. Proteins like integrins are also implicated in these conditions emphasizing the importance of dystonin-related pathways in maintaining cell adhesion and nerve integrity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytoskeletal linker protein. Acts as an integrator of intermediate filaments, actin and microtubule cytoskeleton networks. Required for anchoring either intermediate filaments to the actin cytoskeleton in neural and muscle cells or keratin-containing intermediate filaments to hemidesmosomes in epithelial cells. The proteins may self-aggregate to form filaments or a two-dimensional mesh. Regulates the organization and stability of the microtubule network of sensory neurons to allow axonal transport. Mediates docking of the dynein/dynactin motor complex to vesicle cargos for retrograde axonal transport through its interaction with TMEM108 and DCTN1 (By similarity).. Isoform 3. Plays a structural role in the assembly of hemidesmosomes of epithelial cells; anchors keratin-containing intermediate filaments to the inner plaque of hemidesmosomes. Required for the regulation of keratinocyte polarity and motility; mediates integrin ITGB4 regulation of RAC1 activity.. Isoform 6. Required for bundling actin filaments around the nucleus.. Isoform 7. Regulates the organization and stability of the microtubule network of sensory neurons to allow axonal transport.
See full target information DST
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38612704

2024

Stress-Induced Changes in Nucleocytoplasmic Localization of Crucial Factors in Gene Expression Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Ali Khamit,Payal Chakraborty,Szabolcs Zahorán,Zoltán Villányi,Hajnalka Orvos,Edit Hermesz
View all publications
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Product promise

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