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AB102804

Anti-Dystrophia myotonica protein kinase / DMPK antibody

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Rabbit Polyclonal Dystrophia myotonica protein kinase / DMPK antibody. Carrier free. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human DMPK.

View Alternative Names

DM1PK, MDPK, DMPK, Myotonin-protein kinase, MT-PK, DM-kinase, DM1 protein kinase, Myotonic dystrophy protein kinase, DMK

2 Images
Western blot - Anti-Dystrophia myotonica protein kinase / DMPK antibody (AB102804)
  • WB

Unknown

Western blot - Anti-Dystrophia myotonica protein kinase / DMPK antibody (AB102804)

All lanes:

Western blot - Anti-Dystrophia myotonica protein kinase / DMPK antibody (ab102804) at 1/500 dilution

Lane 1:

Dystrophia myotonica protein kinase / DMPK transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell line at 25 µg

Predicted band size: 70 kDa

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Western blot - Anti-Dystrophia myotonica protein kinase / DMPK antibody (AB102804)
  • WB

Unknown

Western blot - Anti-Dystrophia myotonica protein kinase / DMPK antibody (AB102804)

All lanes:

Western blot - Anti-Dystrophia myotonica protein kinase / DMPK antibody (ab102804) at 1/500 dilution

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 70 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human DMPK.

Q09013

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dystrophia myotonica protein kinase also known as DMPK is a serine-threonine kinase with a molecular mass of approximately 69 kDa. The protein localizes mainly in muscle heart and brain tissues serving critical roles in cellular signaling. DMPK influences various cellular processes through its kinase activity modulating several substrates by phosphorylation. This protein is generally expressed in the cytoplasm but can also localize to membranes influencing ion channel activity and cytoskeletal dynamics.
Biological function summary

DMPK acts to regulate muscle function and integrity by modulating phosphorylation states within cells. It is not typically part of known multi-protein complexes but works alongside other kinases to affect signaling pathways. For example DMPK can influence actin filaments and myosin phosphorylation contributing to muscle cell contractility and stability. This regulatory function is essential for the maintenance of normal muscle tone and function.

Pathways

DMPK participates actively in RhoA and MAPK signaling pathways. DMPK impacts the RhoA pathway by regulating cytoskeletal organization and cell morphology interacting with proteins like Rho kinase to modulate muscle contraction. In the MAPK pathway DMPK plays a role in stress response signaling impacting proteins such as ERK that control gene expression in response to stressors.

DMPK is strongly connected to myotonic dystrophy type 1 (DM1) a genetic disorder characterized by muscle weakness and myotonia. Aberrant expression of DMPK due to triplet repeat expansions in its gene leads to toxic RNA accumulation disrupting normal cellular function. This disorder also shares pathogenic features with MBNL1 where both proteins influence RNA-binding and processing contributing to the DM1 phenotype. Understanding the role of DMPK in these conditions may offer insights into potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Non-receptor serine/threonine protein kinase which is necessary for the maintenance of skeletal muscle structure and function. May play a role in myocyte differentiation and survival by regulating the integrity of the nuclear envelope and the expression of muscle-specific genes. May also phosphorylate PPP1R12A and inhibit the myosin phosphatase activity to regulate myosin phosphorylation. Also critical to the modulation of cardiac contractility and to the maintenance of proper cardiac conduction activity probably through the regulation of cellular calcium homeostasis. Phosphorylates PLN, a regulator of calcium pumps and may regulate sarcoplasmic reticulum calcium uptake in myocytes. May also phosphorylate FXYD1/PLM which is able to induce chloride currents. May also play a role in synaptic plasticity.
See full target information DMPK
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Product promise

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