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AB251665

Anti-Dystrophin antibody

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(1 Publication)

Rabbit Polyclonal Dystrophin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DMD aa 150-350.

View Alternative Names

Dystrophin, DMD

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody (AB251665)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody (AB251665)

Formalin-fixed, paraffin-embedded human heart muscle tissue stained for Dystrophin with ab251665 at a 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody (AB251665)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody (AB251665)

Formalin-fixed, paraffin-embedded human heart muscle tissue shows an absence in immunoreactivity for Dystrophin when incubated with ab251665 at a 1/200 dilution (negative control).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody (AB251665)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody (AB251665)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Dystrophin with ab251665 at a 1/200 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody (AB251665)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody (AB251665)

Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Dystrophin with ab251665 at a 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DMD aa 150-350. The exact immunogen used to generate this antibody is proprietary information.

P11532

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dystrophin also known as the DMD protein plays a mechanical role in muscle fibers by connecting the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. This structural connection helps reinforce the muscle fiber during contraction and mechanical stress. The protein has a molecular weight of approximately 427 kDa. It is expressed mainly in skeletal and cardiac muscles where it is important for maintaining muscle integrity.
Biological function summary

Dystrophin acts as an important component of the dystrophin-glycoprotein complex. This complex stabilizes the muscle cell membrane by linking actin filaments within the cytoskeleton to proteins in the extracellular matrix. The absence or malfunctioning of dystrophin disrupts this connection leading to increased susceptibility to damage during muscle contraction. This is especially evident in tissues where the protein is abundantly present.

Pathways

Dystrophin is integral to the structural integrity pathway in muscle cells. It works alongside proteins like dystroglycan and sarcoglycan forming a multiprotein complex that ensures cell membrane stability during muscle contractions. The proper functioning of the dystrophin complex is also linked to calcium signaling pathways highlighting its role in cellular signaling mechanisms.

Dystrophin's malfunction is directly associated with Duchenne Muscular Dystrophy (DMD) and Becker Muscular Dystrophy (BMD). Mutations in the DMD gene which encodes the dystrophin protein result in the absence or reduced functionality of the protein leading to progressive muscle degeneration observed in DMD and BMD. These disorders frequently involve the protein utrophin which sometimes compensates for the lack of functional dystrophin albeit insufficiently to alleviate the symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission.
See full target information DMD
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 18:2799-2804 PubMed31452758

2019

Fibulin-2 inhibits development of gastric cancer by downregulating β-catenin.

Applications

Unspecified application

Species

Unspecified reactive species

Hongping Ma,Changhong Lian,Yingming Song
View all publications
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Product promise

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