Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (AB275395)

This data was developed using ab275391, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on human skeletal muscle (PMID : 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (AB275395)

This data was developed using ab275391, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on human cardiac muscle (PMID : 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (AB275395)

This data was developed using ab275391, the same antibody clone in a different buffer formulation. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cardiac muscle labelling Cardiac Troponin T with ab307152 at 1/5000 dilution (0.102 µg/mL) (B), CTNNA3 with ab184916 at 1/2000 dilution (0.942 μg/ml) (C) and PDystrophin with ab275391 at 1/500 dilution (2.08 μg/ml) (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Panel A : merged staining of anti-Dystrophin (red; Opal™690), anti-Cardiac troponin T (green; Opal™520) and anti-CTNNA3 (gray; Opal™570) on human cardiac muscle. Panel B : anti-Cardiac troponin T stained on cytoplasm of cardiac muscle. Panel C : anti-CTNNA3 stained on intercalated discs. Panel D : anti-Dystrophin stained on membrane of cardiac muscle. The section was incubated in three rounds of staining : in the order of ab275391, ab307152, and ab184916 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (AB275395)

Fluorescence multiplex immunohistochemical analysis of the human skeletal muscle (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Dystrophin (ab275391, red; Opal™690), anti-A2BP1/Fox1/RBFOX1 (ab254413, green; Opal™520) and anti-Creatine Kinase MM (ab283683, magenta; Opal™570) on human skeletal muscle. Panel B : anti-A2BP1/Fox1/RBFOX1 stained on nucleus of skeletal muscle. Panel C : anti-Creatine Kinase MM stained on cytoplasm of skeletal muscle. Panel D : anti-Dystrophin stained on membrane of skeletal muscle. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab275391 at 1/500 (2.08 μg/ml) dilution, ab254413 at 1/2000 (0.239 μg/ml) dilution, and ab283683 at 1/3000 (0.18 μg/ml) dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. DAPI (blue) was used as a nuclear counter stain. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. This data was developed using ab275391, the same antibody clone in a different buffer formulation.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (AB275395)

Immunohistochemical analysis of formalin fixed paraffin embedded human skeletal muscle labelling Dystrophin with ab275395 at a concentration of 0.1µg/ml. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with a Bond™ Polymer Refine Detection kit. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution 2) for 20mins.

ab275395 anti-Dystrophin antibody [EPR23336-129] was incubated for 15mins at room temperature. Sections were counterstained with Hematoxylin. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (AB275395)

Immunohistochemical analysis of formalin fixed paraffin embedded human skeletal muscle labelling Dystrophin with ab275395 at a concentration of 0.5µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with a OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was performed with DISCOVERY cell conditioning solution (CC1) 100°C, pH8.5 for 32mins.

ab275395 anti-Dystrophin antibody [EPR23336-129] was incubated for 16mins at 37°C. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Customers are encouraged to optimise antigen retrieval conditions, antibody concentration, incubation times and temperature for best results in their own IHC assay workflow (automated and manual)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (AB275395)

This data was developed using ab275391, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on mouse cardiac muscle (PMID : 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (AB275395)

This data was developed using ab275391, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on rat skeletal muscle (PMID : 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (AB275395)

This data was developed using ab275391, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat retina tissue labeling Dystrophin with ab275391 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Positive staining on rat retina. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (AB275395)

This data was developed using ab275391, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded rat cardiac muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on rat cardiac muscle (PMID : 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (AB275395)

This data was developed using ab275391, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue labeling Dystrophin with ab275391 at 1/500 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Membranous staining on mouse skeletal muscle (PMID : 24793134). The section was incubated with ab275391 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX. instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [EPR23336-129] - BSA and Azide free (AB275395)

This data was developed using ab275391, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse retina tissue labeling Dystrophin with ab275391 at 1/100 (5.61 ug/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488) at 1/1000 dilution (Green). Positive staining on mouse retina. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^®488)at 1/1000 dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).