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AB154168

Anti-Dystrophin antibody [EPR9598(ABC)]

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(34 Publications)

Anti-Dystrophin antibody [EPR9598(ABC)] (ab154168) is a rabbit monoclonal antibody detecting Dystrophin in Western Blot. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency
- Over 10 publications

View Alternative Names

Dystrophin, DMD

2 Images
Western blot - Anti-Dystrophin antibody [EPR9598(ABC)] (AB154168)
  • WB

Unknown

Western blot - Anti-Dystrophin antibody [EPR9598(ABC)] (AB154168)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-Dystrophin antibody [EPR9598(ABC)] (ab154168) at 1/1000 dilution

All lanes:

Human fetal skeletal muscle lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 427 kDa

false

Western blot - Anti-Dystrophin antibody [EPR9598(ABC)] (AB154168)
  • WB

Unknown

Western blot - Anti-Dystrophin antibody [EPR9598(ABC)] (AB154168)

All lanes:

Western blot - Anti-Dystrophin antibody [EPR9598(ABC)] (ab154168) at 1/1000 dilution

Lane 1:

Human fetal muscle lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 18 kDa,25 kDa,427 kDa,57 kDa,9 kDa

Observed band size: 18 kDa,25 kDa,90-110 kDa

false

  • Carrier free

    Anti-Dystrophin antibody [EPR9598(ABC)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9598(ABC)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Anti-Dystrophin antibody [EPR9598(ABC)] (ab154168) was developed by Abcam using patented rabbit monoclonal antibody technology and is validated for use in WB.

Abcam's high quality manufacturing and validation processes ensure Anti-Dystrophin antibody [EPR9598(ABC)] (ab154168) has high sensitivity and specificity alongside high lot-to-lot consistency and reproducibility.

Anti-Dystrophin antibody [EPR9598(ABC)] (ab154168) specifically detects Dystrophin (UniProt ID: P11532; Molecular weight: 427kDa) and is sold in 100 µL and 1 mL selling sizes.

Conjugation-ready, carrier free format available for antibody clone EPR9598(ABC) - ab199717.

Dystrophin (DMD) is a crucial protein in neuro research, particularly for its role in maintaining the structural integrity of neurons and muscle cells. It is best known for its association with Duchenne (DMD) and Becker (BMD) muscular dystrophies, where its deficiency leads to progressive muscle degeneration and intellectual disabilities. Researchers use dystrophin to study the molecular mechanisms underlying these disorders and to explore potential therapeutic interventions. Its expression in various brain regions and cell types, including glial cells and neurons, makes it essential for understanding the broader impacts of dystrophin deficiency on the central nervous system.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Dystrophin also known as the DMD protein plays a mechanical role in muscle fibers by connecting the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. This structural connection helps reinforce the muscle fiber during contraction and mechanical stress. The protein has a molecular weight of approximately 427 kDa. It is expressed mainly in skeletal and cardiac muscles where it is important for maintaining muscle integrity.
Biological function summary

Dystrophin acts as an important component of the dystrophin-glycoprotein complex. This complex stabilizes the muscle cell membrane by linking actin filaments within the cytoskeleton to proteins in the extracellular matrix. The absence or malfunctioning of dystrophin disrupts this connection leading to increased susceptibility to damage during muscle contraction. This is especially evident in tissues where the protein is abundantly present.

Pathways

Dystrophin is integral to the structural integrity pathway in muscle cells. It works alongside proteins like dystroglycan and sarcoglycan forming a multiprotein complex that ensures cell membrane stability during muscle contractions. The proper functioning of the dystrophin complex is also linked to calcium signaling pathways highlighting its role in cellular signaling mechanisms.

Dystrophin's malfunction is directly associated with Duchenne Muscular Dystrophy (DMD) and Becker Muscular Dystrophy (BMD). Mutations in the DMD gene which encodes the dystrophin protein result in the absence or reduced functionality of the protein leading to progressive muscle degeneration observed in DMD and BMD. These disorders frequently involve the protein utrophin which sometimes compensates for the lack of functional dystrophin albeit insufficiently to alleviate the symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission.
See full target information DMD
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Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed41002406

2025

Regional Expression of Dystrophin Gene Transcripts and Proteins in the Mouse Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Konstantina Tetorou,Artadokht Aghaeipour,Shunyi Ma,Talia Gileadi,Amel Saoudi,Pablo Perdomo Quinteiro,Jorge Aragón,Maaike van Putten,Pietro Spitali,Cecilia Montanez,Cyrille Vaillend,Jennifer E Morgan,Federica Montanaro,Francesco Muntoni

Molecular therapy. Nucleic acids 36:102665 PubMed40896584

2025

A combinatorial oligonucleotide therapy to improve dystrophin restoration and dystrophin-deficient muscle health.

Applications

Unspecified application

Species

Unspecified reactive species

Young Jae Moon,Ravi Hindupur,Iteoluwakishi H Gamu,Nikki M McCormack,Fatima Shaikh,James S Novak,Jyoti K Jaiswal

Acta neuropathologica communications 13:110 PubMed40400011

2025

A comprehensive spatiotemporal map of dystrophin isoform expression in the developing and adult human brain.

Applications

Unspecified application

Species

Unspecified reactive species

Francesco Catapano,Reem Alkharji,Darren Chambers,Simran Singh,Artadokht Aghaeipour,Jyoti Malhotra,Patrizia Ferretti,Rahul Phadke,Francesco Muntoni

Nucleic acids research 53: PubMed40183632

2025

AOC 1044 induces exon 44 skipping and restores dystrophin protein in preclinical models of Duchenne muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Usue Etxaniz,Isaac Marks,Tyler Albin,Matthew Diaz,Raghav Bhardwaj,Aaron Anderson,Olecya Tyaglo,Tiffany Hoang,Maria Azzurra Missinato,Kristoffer Svensson,Ben Badillo,Philip R Kovach,Laura Leung,Michael Cochran,Hae Won Kwon,Md Nur Ahad Shah,Rika Maruyama,Toshifumi Yokota,Venkata R Doppalapudi,Beatrice Darimont,Husam S Younis,W Michael Flanagan,Arthur A Levin,Hanhua Huang,Georgios Karamanlidis

International journal of molecular sciences 26: PubMed40141224

2025

Valproic Acid Improves Antisense-Mediated Exon-Skipping Efficacy in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Micky Phongsavanh,Flavien Bizot,Amel Saoudi,Cecile Gastaldi,Olivier Le Coz,Thomas Tensorer,Elise Brisebard,Luis Garcia,Aurélie Goyenvalle

Skeletal muscle 14:28 PubMed39614336

2024

Golodirsen restores DMD transcript imbalance in Duchenne Muscular Dystrophy patient muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rachele Rossi,Silvia Torelli,Marc Moore,Pierpaolo Ala,Jennifer Morgan,Jyoti Malhotra,Francesco Muntoni

Journal of medicinal chemistry 67:14868-14884 PubMed39197837

2024

Structure-Activity Relationship of Antibody-Oligonucleotide Conjugates: Evaluating Bioconjugation Strategies for Antibody-Phosphorodiamidate Morpholino Oligomer Conjugates for Drug Development.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Cochran,Isaac Marks,Tyler Albin,Danny Arias,Philip Kovach,Beatrice Darimont,Hanhua Huang,Usue Etxaniz,Hae Won Kwon,Yunyu Shi,Matthew Diaz,Olecya Tyaglo,Arthur Levin,Venkata Ramana Doppalapudi

Molecular therapy. Nucleic acids 35:102228 PubMed38975000

2024

Deletion of miR-146a enhances therapeutic protein restoration in model of dystrophin exon skipping.

Applications

Unspecified application

Species

Unspecified reactive species

Nikki M McCormack,Kelsey A Calabrese,Christina M Sun,Christopher B Tully,Christopher R Heier,Alyson A Fiorillo

Molecular therapy : the journal of the American Society of Gene Therapy 32:2604-2623 PubMed38910327

2024

Dual FKRP/FST gene therapy normalizes ambulation, increases strength, decreases pathology, and amplifies gene expression in LGMDR9 mice.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia Lam,Deborah A Zygmunt,Anna Ashbrook,Macey Bennett,Tatyana A Vetter,Paul T Martin

Biomedicines 11: PubMed38137463

2023

Networking to Optimize exon 53 Skipping in the Brain of Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Mathilde Doisy,Ophélie Vacca,Claire Fergus,Talia Gileadi,Minou Verhaeg,Amel Saoudi,Thomas Tensorer,Luis Garcia,Vincent P Kelly,Federica Montanaro,Jennifer E Morgan,Maaike van Putten,Annemieke Aartsma-Rus,Cyrille Vaillend,Francesco Muntoni,Aurélie Goyenvalle
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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