Anti-Dystrophin antibody [RM1069]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [RM1069] (AB315102)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Dystrophin with ab315102 at 1/200 (2.52 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Membrane staining on human cardiac muscle. The section was incubated with ab315102 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [RM1069] (AB315102)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Dystrophin with ab315102 at 1/100 (5.04 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

Low expression : confocal image showing no staining on rat liver (PMID : 9419360). The nuclear counterstain was DAPI (Blue). The section was incubated with ab315102 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [RM1069] (AB315102)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Dystrophin with ab315102 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse liver. The section was incubated with ab315102 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [RM1069] (AB315102)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Dystrophin with ab315102 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Membrane staining on mouse cardiac muscle. The section was incubated with ab315102 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [RM1069] (AB315102)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Dystrophin with ab315102 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Membrane staining on rat cardiac muscle. The section was incubated with ab315102 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [RM1069] (AB315102)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cardiac muscle (fresh) tissue labeling Dystrophin with ab315102 at 1/100 (5.04 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining on rat cardiac muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab315102 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [RM1069] (AB315102)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cardiac muscle (fresh) tissue labeling Dystrophin with ab315102 at 1/100 (5.04 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing positive staining on mouse cardiac muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab315102 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [RM1069] (AB315102)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Dystrophin with ab315102 at 1/100 (5.04 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).

Low expression : confocal image showing no staining on mouse liver (PMID : 9419360). The nuclear counterstain was DAPI (Blue). The section was incubated with ab315102 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.

• IP

Supplier Data

Immunoprecipitation - Anti-Dystrophin antibody [RM1069] (AB315102)

Dystrophin was immunoprecipitated from 0.35 mg Rat skeletal muscle tissue lysate with ab315102 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315102 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat skeletal muscle tissue lysate

Lane 2 : ab315102 IP in Rat skeletal muscle tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315102 in rat skeletal muscle tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Dystrophin antibody [RM1069] (ab315102) at 1/30 dilution

All lanes:

Rat skeletal muscle tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 15s

• IP

Supplier Data

Immunoprecipitation - Anti-Dystrophin antibody [RM1069] (AB315102)

Dystrophin was immunoprecipitated from 0.35 mg Mouse heart tissue lysate with ab315102 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315102 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse heart tissue lysate

Lane 2 : ab315102 IP in Mouse heart tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab315102 in mouse heart tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Dystrophin antibody [RM1069] (ab315102) at 1/30 dilution

All lanes:

Mouse heart tissue lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 15s

• WB

Supplier Data

Western blot - Anti-Dystrophin antibody [RM1069] (AB315102)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver (PMID : 1319059).

The molecular weight observed is consistent with what have been described in literature (PMID : 7633443, 9224291, 10675908).

Other weak immunoreactive bands may indicate degradation products (PMID : 9224291).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Dystrophin antibody [RM1069] (ab315102) at 1/1000 dilution

Lane 1:

Human skeletal muscle tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 260 kDa,420 kDa,124 kDa

true

Exposure time: 70s

• WB

Supplier Data

Western blot - Anti-Dystrophin antibody [RM1069] (AB315102)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : liver and kidney (PMID : 1319059).

The molecular weight observed is consistent with what have been described in literature (PMID : 7633443, 9224291, 10675908).

Other weak immunoreactive bands may indicate degradation products (PMID : 9224291).

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-Dystrophin antibody [RM1069] (ab315102) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse kidney tissue lysate at 20 µg

Lane 4:

Rat skeletal muscle tissue lysate at 20 µg

Lane 5:

Rat heart tissue lysate at 20 µg

Lane 6:

Rat liver tissue lysate at 20 µg

Lane 7:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 420 kDa,260 kDa,124 kDa

true

Exposure time: 15s