Anti-Dystrophin antibody [RM1069] (ab315102)

Rabbit Recombinant Multiclonal Dystrophin antibody. Suitable for WB, IP, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples.

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Images

Immunoprecipitation - Anti-Dystrophin antibody [RM1069] (AB315102), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IP	IHC-P	IHC-Fr	ICC/IF
Human	Tested	Expected	Tested	Expected	Not recommended
Mouse	Tested	Tested	Tested	Tested	Not recommended
Rat	Tested	Tested	Tested	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes -
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes -
Species Rat	Dilution info 1/30	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/200	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Mouse	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/5000	Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Rat	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human, Mouse, Rat	Dilution info -	Notes -

Associated Products

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1 product for Alternative Version

Target data

See full target information DMD

Function

Anchors the extracellular matrix to the cytoskeleton via F-actin. Ligand for dystroglycan. Component of the dystrophin-associated glycoprotein complex which accumulates at the neuromuscular junction (NMJ) and at a variety of synapses in the peripheral and central nervous systems and has a structural function in stabilizing the sarcolemma. Also implicated in signaling events and synaptic transmission.

Additional Targets

Dmd

Alternative names

Dystrophin, DMD

Recommended products

Rabbit Recombinant Multiclonal Dystrophin antibody. Suitable for WB, IP, IHC-P, IHC-Fr and reacts with Mouse, Rat, Human samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Multiclonal
Immunogens: The exact immunogen used to generate this antibody is proprietary information.
Clone number: RM1069
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Dystrophin also known as the DMD protein plays a mechanical role in muscle fibers by connecting the cytoskeleton of a muscle fiber to the surrounding extracellular matrix through the cell membrane. This structural connection helps reinforce the muscle fiber during contraction and mechanical stress. The protein has a molecular weight of approximately 427 kDa. It is expressed mainly in skeletal and cardiac muscles where it is important for maintaining muscle integrity.

Biological function summary

Dystrophin acts as an important component of the dystrophin-glycoprotein complex. This complex stabilizes the muscle cell membrane by linking actin filaments within the cytoskeleton to proteins in the extracellular matrix. The absence or malfunctioning of dystrophin disrupts this connection leading to increased susceptibility to damage during muscle contraction. This is especially evident in tissues where the protein is abundantly present.

Pathways

Dystrophin is integral to the structural integrity pathway in muscle cells. It works alongside proteins like dystroglycan and sarcoglycan forming a multiprotein complex that ensures cell membrane stability during muscle contractions. The proper functioning of the dystrophin complex is also linked to calcium signaling pathways highlighting its role in cellular signaling mechanisms.

Associated diseases and disorders

Dystrophin's malfunction is directly associated with Duchenne Muscular Dystrophy (DMD) and Becker Muscular Dystrophy (BMD). Mutations in the DMD gene which encodes the dystrophin protein result in the absence or reduced functionality of the protein leading to progressive muscle degeneration observed in DMD and BMD. These disorders frequently involve the protein utrophin which sometimes compensates for the lack of functional dystrophin albeit insufficiently to alleviate the symptoms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

12 product images

Immunoprecipitation - Anti-Dystrophin antibody [RM1069] (ab315102)
Dystrophin was immunoprecipitated from 0.35 mg Rat skeletal muscle tissue lysate with ab315102 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315102 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Rat skeletal muscle tissue lysate
Lane 2: ab315102 IP in Rat skeletal muscle tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab315102 in rat skeletal muscle tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Dystrophin antibody [RM1069] (ab315102) at 1/30 dilution
All lanes: Rat skeletal muscle tissue lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 15s
Immunoprecipitation - Anti-Dystrophin antibody [RM1069] (ab315102)
Dystrophin was immunoprecipitated from 0.35 mg Mouse heart tissue lysate with ab315102 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab315102 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Mouse heart tissue lysate
Lane 2: ab315102 IP in Mouse heart tissue lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab315102 in mouse heart tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Dystrophin antibody [RM1069] (ab315102) at 1/30 dilution
All lanes: Mouse heart tissue lysate
Secondary
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Exposure time: 15s
Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [RM1069] (ab315102)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver (fresh) tissue labeling Dystrophin with ab315102 at 1/100 (5.04 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).
Low expression: confocal image showing no staining on rat liver (PMID: 9419360). The nuclear counterstain was DAPI (Blue). The section was incubated with ab315102 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [RM1069] (ab315102)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat cardiac muscle (fresh) tissue labeling Dystrophin with ab315102 at 1/100 (5.04 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing positive staining on rat cardiac muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab315102 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [RM1069] (ab315102)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (fresh) tissue labeling Dystrophin with ab315102 at 1/100 (5.04 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).
Low expression: confocal image showing no staining on mouse liver (PMID: 9419360). The nuclear counterstain was DAPI (Blue). The section was incubated with ab315102 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-Dystrophin antibody [RM1069] (ab315102)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cardiac muscle (fresh) tissue labeling Dystrophin with ab315102 at 1/100 (5.04 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing positive staining on mouse cardiac muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab315102 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [RM1069] (ab315102)
Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Dystrophin with ab315102 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on mouse liver. The section was incubated with ab315102 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [RM1069] (ab315102)
Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Dystrophin with ab315102 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Membrane staining on rat cardiac muscle. The section was incubated with ab315102 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [RM1069] (ab315102)
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Dystrophin with ab315102 at 1/5000 (0.101 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Membrane staining on mouse cardiac muscle. The section was incubated with ab315102 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dystrophin antibody [RM1069] (ab315102)
Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling Dystrophin with ab315102 at 1/200 (2.52 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Membrane staining on human cardiac muscle. The section was incubated with ab315102 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins
Western blot - Anti-Dystrophin antibody [RM1069] (ab315102)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: liver (PMID: 1319059).
The molecular weight observed is consistent with what have been described in literature (PMID: 7633443, 9224291, 10675908).
Other weak immunoreactive bands may indicate degradation products (PMID: 9224291).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Dystrophin antibody [RM1069] (ab315102) at 1/1000 dilution
Lane 1: Human skeletal muscle tissue lysate at 20 µg
Lane 2: Human liver tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 420, 260 kDa, 124 kDa
Exposure time: 70s
Western blot - Anti-Dystrophin antibody [RM1069] (ab315102)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: liver and kidney (PMID: 1319059).
The molecular weight observed is consistent with what have been described in literature (PMID: 7633443, 9224291, 10675908).
Other weak immunoreactive bands may indicate degradation products (PMID: 9224291).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-Dystrophin antibody [RM1069] (ab315102) at 1/1000 dilution
Lane 1: Mouse skeletal muscle tissue lysate at 20 µg
Lane 2: Mouse liver tissue lysate at 20 µg
Lane 3: Mouse kidney tissue lysate at 20 µg
Lane 4: Rat skeletal muscle tissue lysate at 20 µg
Lane 5: Rat heart tissue lysate at 20 µg
Lane 6: Rat liver tissue lysate at 20 µg
Lane 7: Rat kidney tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 420, 260 kDa, 124 kDa
Exposure time: 15s