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Mouse Recombinant Monoclonal E Cadherin antibody. Carrier free. Suitable for WB and reacts with Human samples.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (AB287971), expandable thumbnail
  • Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (AB287971), expandable thumbnail
  • Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (AB287971), expandable thumbnail

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-P
Human
Tested
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.

Alternative names

CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin

Recommended products

Mouse Recombinant Monoclonal E Cadherin antibody. Carrier free. Suitable for WB and reacts with Human samples.

Key facts

Isotype
IgG2a
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
36/E-Cadherin
Purification technique
Affinity purification Protein A
Specificity

This antibody recognises overexpressed P-cadherin and may have some degree of cross-reactivity with endogenous P-caderin

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

This antibody is a carrier free version of Anti-E Cadherin antibody [36/E-Cadherin] ab287970.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

Associated diseases and disorders

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)

    This data was developed using Anti-E Cadherin antibody [36/E-Cadherin] ab287970, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) whole cell lysate labeling E Cadherin with Anti-E Cadherin antibody [36/E-Cadherin] ab287970 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in MCF7 cell line. The nuclear counter stain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-beta Tubulin antibody [EPR16774] ab179513) at 1/200 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) secondary antibody at 1/500 dilution (red).
    The negative controls are as follows:
    -ve control 1: Anti-E Cadherin antibody [36/E-Cadherin] ab287970 at 1/2000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080) secondary antibody at 1/500 dilution.
    -ve control 2: Anti-alpha Tubulin mouse MAb (Anti-beta Tubulin antibody [EPR16774] ab179513) at 1/200 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed ab150117) secondary antibody at 1/1000 dilution.

  • Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971), expandable thumbnail

    Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)

    This data was developed using Anti-E Cadherin antibody [36/E-Cadherin] ab287970, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as blocking and diluting buffer.

    Full-length E-cadherin is ~120 kDa. The other bands are due to proteolytic cleavage in different Cadherin domains (PMID: 11112695; PMID: 11212238).

    All lanes: Western blot - Anti-E Cadherin antibody [36/E-Cadherin] (Anti-E Cadherin antibody [36/E-Cadherin] ab287970) at 1/1000 dilution

    Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Lane 2: MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 97 kDa

    Observed band size: 110 kDa, 120 kDa

    Exposure time: 7s

  • Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971), expandable thumbnail

    Western blot - Anti-E Cadherin antibody [36/E-Cadherin] - BSA and Azide free (ab287971)

    This data was developed using Anti-E Cadherin antibody [36/E-Cadherin] ab287970, the same antibody clone in a different buffer formulation.

    5% NFDM/TBST was used as blocking and diluting buffer.

    This antibody has cross-reactivity with P-Cadherin.

    All lanes: Western blot - Anti-E Cadherin antibody [36/E-Cadherin] (Anti-E Cadherin antibody [36/E-Cadherin] ab287970) at 1/1000 dilution

    Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 2: HEK-293T transfected with P-Cadherin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Lane 3: HEK-293T transfected with E-Cadherin expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/10000 dilution

    Predicted band size: 97 kDa

    Observed band size: 120 kDa

    Exposure time: 3s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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