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Rabbit Recombinant Monoclonal E Cadherin antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063), expandable thumbnail
  • Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063), expandable thumbnail
  • Immunoprecipitation - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063), expandable thumbnail
  • Flow Cytometry - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063), expandable thumbnail
  • Immunocytochemistry - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information. Database link P12830

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICCIHC-PIPWB
Human
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Expected
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

CDH1 encodes a protein that is a calcium-dependent cell adhesion molecule, playing a role in cell-cell adhesion, mobility, and proliferation in epithelial cells. It acts as a potent invasive suppressor and serves as a ligand for integrin alpha-E/beta-7. The protein contributes to the sorting of heterogeneous cell types due to its homophilic interactions. E-Cad/CTF2, a product of CDH1, promotes the non-amyloidogenic degradation of Abeta precursors and strongly inhibits the production of APP C99 and C83. Additionally, in the context of microbial infection, it functions as a receptor for Listeria monocytogenes, with internalin A binding to it to promote bacterial uptake. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin

Recommended products

Rabbit Recombinant Monoclonal E Cadherin antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information. Database link P12830
Clone number
BLR088G
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

Associated diseases and disorders

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063), expandable thumbnail

    Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063)

    Samples: Whole cell lysate (5 µg) from GaMG, HCT 116, U2OS, OVCAR-4, Jurkat, 22Rv1, HeLa, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody:Rabbit anti-ECadherin recombinant monoclonal antibody [BLR088G] (A700-088 lot 1) used at 1:1000. Secondary:HRPconjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (A700-057).

    All lanes: Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063) at 1/1000 dilution

    Lane 1: GaMG Whole cell lysate, at 5 µg

    Lane 2: HCT 116 Whole cell lysate, at 5 µg

    Lane 3: U2OS Whole cell lysate at 5 µg

    Lane 4: OVCAR-4 Whole cell lysate at 5 µg

    Lane 5: Jurkat Whole cell lysate at 5 µg

    Lane 6: 22Rv1 Whole cell lysate at 5 µg

    Lane 7: HeLa Whole cell lysate at 5 µg

    Lane 8: MCF-7 Whole cell lysate at 5 µg

    Lane 9: K-562 Whole cell lysate at 5 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063), expandable thumbnail

    Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063)

    Samples: Whole cell lysate (5 µg) from EL4, mIMCD-3, NIH 3T3, Renca, and TCMK-1 cells prepared using NETN lysis buffer. Antibody:Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] (A700-088 lot 1) used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.

    All lanes: Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063) at 1/1000 dilution

    Lane 1: EL4 Whole cell lysate at 5 µg

    Lane 2: mIMCD-3 Whole cell lysate at 5 µg

    Lane 3: NIH 3T3 Whole cell lysate at 5 µg

    Lane 4: Renca Whole cell lysate at 5 µg

    Lane 5: TCMK-1 Whole cell lysate at 5 µg

    Secondary

    All lanes: HRP-conjugated goat anti-rabbit IgG

  • Immunoprecipitation - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063), expandable thumbnail

    Immunoprecipitation - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063)

    Samples:Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies:Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] used for IP at 20 µl/mg lysate. E-Cadherin was also immunoprecipitated by rabbit anti-E-Cadherin antibody (BL13080). For blotting immunoprecipitated E-Cadherin, was used at 1:1000. Chemiluminescence with an exposure time of 1 second.

  • Flow Cytometry - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063), expandable thumbnail

    Flow Cytometry - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063)

    Detection of human E-Cadherin (shaded) in HT29 cells by flow cytometry.
    Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G]  or isotype control (unshaded). Secondary:  650-conjugated goat anti-rabbit IgG

  • Immunocytochemistry - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063), expandable thumbnail

    Immunocytochemistry - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063)

    Detection of human E-Cadherin in FFPE HCT-116 cells by ICC Antibody:Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] (A700-088 lot 1). Secondary:HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate:DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063)

    Detection of human E-Cadherin in FFPE squamous cell carcinoma by IHC Antibody:Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] (A700-088 lot 1). Secondary:HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate:DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063)

    Detection of mouse E-Cadherin in FFPE skin by IHC. Antibody:Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] (A700-088 lot 1). Secondary:HRPconjugated goat anti-rabbit IgG (A120-501P). Substrate: DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063)

    Detection of human E-Cadherin in FFPE lung carcinoma by IHC. Antibody:Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] (A700-088 lot 1). Secondary:HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate:DAB.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063)

    Detection of human E-Cadherin in FFPE lung carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] (A700-088 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate:Opal™. Counterstain: DAPI.

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