AB314063

Anti-E-Cadherin antibody [BLR088G] - BSA free

Recombinant
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(26 Publications)

Rabbit Recombinant Monoclonal E Cadherin antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Human, Mouse samples. Cited in 26 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CDH1 aa 150-750.

View Alternative Names

CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin

9 Images

Flow Cytometry - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Detection of human E-Cadherin (shaded) in HT29 cells by flow cytometry. Antibody : Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] or isotype control (unshaded). Secondary : DyLight^® 650-conjugated goat anti-rabbit IgG (ab96886).

Supplier Data

Immunocytochemistry - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Detection of human E-Cadherin in FFPE HCT-116 cells by ICC Antibody : Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] (A700-088 lot 1). Secondary : HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate : DAB.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Detection of human E-Cadherin in FFPE lung carcinoma by immunohistochemistry-IF. Antibody : Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] (A700-088 Lot 1). Secondary : HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate : Opal™. Counterstain : DAPI.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Detection of human E-Cadherin in FFPE squamous cell carcinoma by IHC Antibody : Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] (A700-088 lot 1). Secondary : HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate : DAB.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Detection of human E-Cadherin in FFPE lung carcinoma by IHC. Antibody : Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] (A700-088 lot 1). Secondary : HRP-conjugated goat anti-rabbit IgG (A120-501P). Substrate : DAB.

Supplier Data

Immunoprecipitation - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] used for IP at 20 µl/mg lysate. E-Cadherin was also immunoprecipitated by rabbit anti-E-Cadherin antibody (BL13080). For blotting immunoprecipitated E-Cadherin, was used at 1 : 1000. Chemiluminescence with an exposure time of 1 second.

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IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Detection of mouse E-Cadherin in FFPE skin by IHC. Antibody : Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] (A700-088 lot 1). Secondary : HRPconjugated goat anti-rabbit IgG (A120-501P). Substrate : DAB.

Supplier Data

Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Samples : Whole cell lysate (5 µg) from GaMG, HCT 116, U2OS, OVCAR-4, Jurkat, 22Rv1, HeLa, MCF-7, and K-562 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-ECadherin recombinant monoclonal antibody [BLR088G] (A700-088 lot 1) used at 1 : 1000. Secondary : HRPconjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (A700-057).

All lanes:

Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063) at 1/1000 dilution

Lane 1:

GaMG Whole cell lysate, at 5 µg

Lane 2:

HCT 116 Whole cell lysate, at 5 µg

Lane 3:

U2OS Whole cell lysate at 5 µg

Lane 4:

OVCAR-4 Whole cell lysate at 5 µg

Lane 5:

Jurkat Whole cell lysate at 5 µg

Lane 6:

22Rv1 Whole cell lysate at 5 µg

Lane 7:

HeLa Whole cell lysate at 5 µg

Lane 8:

MCF-7 Whole cell lysate at 5 µg

Lane 9:

K-562 Whole cell lysate at 5 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Supplier Data

Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Samples : Whole cell lysate (5 µg) from EL4, mIMCD-3, NIH 3T3, Renca, and TCMK-1 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-E-Cadherin recombinant monoclonal antibody [BLR088G] (A700-088 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (ab314063) at 1/1000 dilution

Lane 1:

EL4 Whole cell lysate at 5 µg

Lane 2:

mIMCD-3 Whole cell lysate at 5 µg

Lane 3:

NIH 3T3 Whole cell lysate at 5 µg

Lane 4:

Renca Whole cell lysate at 5 µg

Lane 5:

TCMK-1 Whole cell lysate at 5 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR088G

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

WB, IHC-P, ICC, IP, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human CDH1 aa 150-750. The exact immunogen used to generate this antibody is proprietary information.

P12830

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed : 29999492). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.

See full target information CDH1

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Publications (26)

Recent publications for all applications. Explore the full list and refine your search

Oncology research 33:2379-2398 PubMed40918458

2025

Suppresses Colorectal Cancer Progression by Inhibiting Epithelial-Mesenchymal Transition via Regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Yuhang Jiang,Yijun Xu,Qi Zhu,Yingxia Wu,Zhe Wang,Shuang He,Shiyong Yu,Honggang Xiang

Scientific reports 15:29838 PubMed40813603

2025

A novel neutrophil extracellular trap-related gene signature for predicting glioblastoma prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Huang,Xue-Bin Yu,Yong-Zhi Zhou

Journal of ovarian research 18:180 PubMed40790593

2025

Oleic acid activates TGFβ-Smad3 signaling to promote ovarian cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyang Guo,Yinjia Li,Yunyun Guo,Aosong Zhang,Xiao Huo,Ying Song,Bing Li,Yuanjun Tang,Tianhui He,Tong Liu,Lixiang Xue,Yi Qu,Jiagui Song

Cell death & disease 16:597 PubMed40775202

2025

ULBP2 CAR-T cells enhance gastric cancer immunotherapy by inhibiting CAF activation.

Applications

Unspecified application

Species

Unspecified reactive species

Wentao Zhang,Wen Ren,Shuyan Guo,Haobo Han,Weiwen Cai,Haowen Bai,Long Li,Xiangyan Jiang,Xin Zheng,Tiansheng Zhang,Yan Wang,Huili Ye,Hongtai Cao,Wengui Shi,Huinian Zhou,Zeyuan Yu,Long Qin,Zuoyi Jiao

Cell division 20:20 PubMed40751214

2025

SMAD3 orchestrates RNF167 and STAMBPL1-mediated Sestrin2 ubiquitination to drive gastric cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Zhang,Na Zhao,Hainan Zhang,Le Yao,Hongtao Si

Journal of cell communication and signaling 19:e70037 PubMed40717692

2025

Cancer-associated fibroblasts-secreted lactate promotes RNA polymerase III subunit G-mediated epithelial-mesenchymal transition in non-small cell lung cancer by increasing m6A modification of zinc finger protein 384.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Li,Xing Yang,Hao Tang,Zhiping Zhou,Bin Liu

Molecular medicine reports 32: PubMed40641129

2025

AZD1080, a specific inhibitor of GSK‑3β, inhibits stemness and malignancies in osteosarcoma cancer stem‑like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peiyu Guo,Zhenkai Lou,Hongda Gong,Xiaodong Hou,Chunqiang Zhang,Bing Wang,Kaili Du

International journal of nanomedicine 20:8063-8083 PubMed40626139

2025

Macrophage Membrane-Coated Liposomes Delivering Vonoprazan Disrupt Mitochondrial Oxidative Phosphorylation in Diffuse Large B-Cell Lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Wang,Lei Wang,Qingqing Fan,Jingyan Xu,Yifan Li,Jiale Xu,Bei Zhao,Dan Ge,Longying Xiong,Yanping Wu,Yun Zhu,Min Chen

Scientific reports 15:21635 PubMed40594164

2025

Hypoxia-induced HIF-1α/VASN promotes bladder cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Qian-Jin Zhang,Chun-Hui Liu,Ke Wang,Xue-Yuan Mao,Yang Dong,Ming-Yi Zang,Wei Zhang,Quan-Sheng Yu,Lin Hao

Materials today. Bio 32:101818 PubMed40453816

2025

Matrix-bound EGF promotes malignant phenotypes of breast cancer organoids in the biomimetic ECM of alginate.

Applications

Unspecified application

Species

Unspecified reactive species

Xue-Yu Chen,Meng-Yuan Wang,Xin Shu,Jun Li,Ruizhi Tang,Xi-Qiu Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-E-Cadherin antibody [BLR088G] - BSA free

Flow Cytometry - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Immunocytochemistry - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Immunoprecipitation - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Western blot - Anti-E-Cadherin antibody [BLR088G] - BSA free (AB314063)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (26)

Product promise