AB219332

Anti-E Cadherin antibody [CDH1/1525]

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(18 Publications)

Mouse Monoclonal E Cadherin antibody. Suitable for ICC, WB, IHC-P and reacts with Human, Recombinant fragment - Human samples. Cited in 18 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDH1.

View Alternative Names

CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Immunohistochemical analysis of formalin fixed paraffin embedded human colon carcinoma tissue labeling E Cadherin with ab219332 at 2 μg/mL.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Hepatocellular Carcinoma tissue labelling E Cadherin with ab219332 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Immunohistochemical analysis of formalin fixed paraffin embedded human placenta tissue labeling E Cadherin with ab219332 at 2 μg/mL.

Supplier Data

Immunocytochemistry - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Immunofluorescence analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling E Cadherin with ab219332 at 2 µg/mL (green). The nuclear counterstain is Reddot (red).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Immunohistochemical analysis of formalin fixed paraffin embedded human colon carcinoma tissue labeling E Cadherin with ab219332 at 2 μg/mL.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Immunohistochemical analysis of formalin fixed paraffin embedded human prostate carcinoma tissue labeling E Cadherin with ab219332 at 2 μg/mL.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Immunohistochemical analysis of formalin fixed, paraffin embedded human skin tissue labeling E Cadherin with ab219332 at 2 μg/mL.

Supplier Data

Western blot - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

All lanes:

Western blot - Anti-E Cadherin antibody [CDH1/1525] (ab219332) at 1 µg/mL

Lane 1:

Recombinant E-Cadherin protein

Lane 2:

Human stomach lysate

Predicted band size: 97 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CDH1/1525

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

WB, ICC, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CDH1.

P12830

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "" }, "Mouse": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "" }, "Rat": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "1-2 µg/mL", "ICC-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "ICC-species-checked": "notRecommended", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed : 29999492). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.

See full target information CDH1

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:136 PubMed40082910

2025

Basal cell adhesion molecule (BCAM) promotes mesothelial-to-mesenchymal transition and tumor angiogenesis through paracrine signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Suresh Sivakumar,Sonja Lieber,Raimund Dietze,Vanessa M Beutgen,Eileen C Sutor,Sophie Heidemann,Florian Finkernagel,Julia Teply-Szymanski,Andrea Nist,Thorsten Stiewe,Katrin Roth,Silke Reinartz,Johannes Graumann,Sabine Müller-Brüsselbach,Rolf Müller

Cell communication and signaling : CCS 22:46 PubMed38233791

2024

ZDHHC5-mediated S-palmitoylation of FAK promotes its membrane localization and epithelial-mesenchymal transition in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Wang,Na Shen,Yang Yang,Yuan Xia,Wenhao Zhang,Yu Lu,Zhicheng Wang,Ze Yang,Zhangjie Wang

Frontiers in medicine 10:1158359 PubMed37384048

2023

Renal glomerular and tubular responses to glutaraldehyde- polymerized human hemoglobin.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew C Williams,Xiaoyuan Zhang,Jin Hyen Baek,Felice D'Agnillo

Nature communications 14:3393 PubMed37296125

2023

Intranasal or airborne transmission-mediated delivery of an attenuated SARS-CoV-2 protects Syrian hamsters against new variants.

Applications

Unspecified application

Species

Unspecified reactive species

Charles B Stauft,Prabhuanand Selvaraj,Felice D'Agnillo,Clement A Meseda,Shufeng Liu,Cyntia L Pedro,Kotou Sangare,Christopher Z Lien,Jerry P Weir,Matthew F Starost,Tony T Wang

Journal of cellular and molecular medicine 26:5657-5669 PubMed36282889

2022

Identifying cancer cell-secreted proteins that activate cancer-associated fibroblasts as prognostic factors for patients with pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qiankun Luo,Jiayin Liu,Qiang Fu,Xu Zhang,Pengfei Yu,Pan Liu,Jiali Zhang,Huiyuan Tian,Song Chen,Hongwei Zhang,Tao Qin

Journal of digestive diseases 23:376-387 PubMed35844201

2022

Circular RNA circ_0006089 promotes the progression of gastric cancer by regulating the miR-143-3p/PTBP3 axis and PI3K/AKT signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Rong Lin,Wei Rong Chen,Pei Hong Zheng,Wei Shan Chen,Gao Yang Cai

Folia neuropathologica 60:195-209 PubMed35950472

2022

Effects of miR-211-3p/RHBDD1 axis on cell proliferation, cell cycle progression, and epithelial-mesenchymal transition in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Chen,Xiangyi Wang,Yude Zhu,Fuquan Liu,Laixing Liu,Ning Jiang

Cellular & molecular biology letters 27:39 PubMed35578166

2022

Identification and validation of an eight-lncRNA signature that predicts prognosis in patients with esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jinfeng Zhang,Xiaodong Ling,Chengyuan Fang,Jianqun Ma

Diabetology & metabolic syndrome 13:108 PubMed34654473

2021

LncRNA KCNQ1OT1 promotes the development of diabetic nephropathy by regulating miR-93-5p/ROCK2 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Li Zhao,Huaqian Chen,Lin Wu,Zhengdong Li,Ren Zhang,Yan Zeng,Tao Yang,Hualing Ruan

Oncology reports 45: PubMed33907830

2021

Schisandrin B inhibits epithelial‑mesenchymal transition and stemness of large‑cell lung cancer cells and tumorigenesis in xenografts via inhibiting the NF‑κB and p38 MAPK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Shuping Li,Hong Wang,Ruidong Ma,Li Wang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-E Cadherin antibody [CDH1/1525]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Immunocytochemistry - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Western blot - Anti-E Cadherin antibody [CDH1/1525] (AB219332)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (18)

Product promise