Anti-E Cadherin antibody [CDH1/1525] (ab219332)

Mouse Monoclonal E Cadherin antibody. Suitable for ICC, WB, IHC-P and reacts with Human, Recombinant fragment - Human samples. Cited in 15 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDH1.

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Images

Immunocytochemistry - Anti-E Cadherin antibody [CDH1/1525] (AB219332), expandable thumbnail

Publications

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

Recombinant Full Length Protein corresponding to Human CDH1. Database link P12830

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	ICC	WB	IHC-P
Human	Tested	Expected	Tested
Mouse	Not recommended	Not recommended	Not recommended
Rat	Not recommended	Not recommended	Not recommended
Recombinant fragment - Human	Not recommended	Tested	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1-2 µg/mL	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes -
Species Recombinant fragment - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Recombinant fragment - Human	Dilution info 1-2 µg/mL	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1-2 µg/mL	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Recombinant fragment - Human	Dilution info -	Notes -

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Target data

See full target information CDH1

Function

CDH1 encodes a protein that is a calcium-dependent cell adhesion molecule, playing a role in cell-cell adhesion, mobility, and proliferation in epithelial cells. It acts as a potent invasive suppressor and serves as a ligand for integrin alpha-E/beta-7. The protein contributes to the sorting of heterogeneous cell types due to its homophilic interactions. E-Cad/CTF2, a product of CDH1, promotes the non-amyloidogenic degradation of Abeta precursors and strongly inhibits the production of APP C99 and C83. Additionally, in the context of microbial infection, it functions as a receptor for Listeria monocytogenes, with internalin A binding to it to promote bacterial uptake. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin

Key facts

Isotype: IgG1
Host species: Mouse
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: Recombinant Full Length Protein corresponding to Human CDH1. Database link P12830
Clone number: CDH1/1525
Purification technique: Affinity purification Protein G
Light chain type: kappa
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

Associated diseases and disorders

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

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8 product images

Immunocytochemistry - Anti-E Cadherin antibody [CDH1/1525] (ab219332)
Immunofluorescence analysis of MCF7 (human breast adenocarcinoma cell line) cells labeling E Cadherin with ab219332 at 2 µg/mL (green). The nuclear counterstain is Reddot (red).
Western blot - Anti-E Cadherin antibody [CDH1/1525] (ab219332)
All lanes: Western blot - Anti-E Cadherin antibody [CDH1/1525] (ab219332) at 1 µg/mL
Lane 1: Recombinant E-Cadherin protein
Lane 2: Human stomach lysate
Predicted band size: 97 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Hepatocellular Carcinoma tissue labelling E Cadherin with ab219332 at 2 μg/mL for 30 minutes at room temperature. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at room temperature for 20 minutes.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)
Immunohistochemical analysis of formalin fixed, paraffin embedded human colon carcinoma tissue labeling E Cadherin with ab219332 at 2 μg/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)
Immunohistochemical analysis of formalin fixed, paraffin embedded human skin tissue labeling E Cadherin with ab219332 at 2 μg/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)
Immunohistochemical analysis of formalin fixed, paraffin embedded human prostate carcinoma tissue labeling E Cadherin with ab219332 at 2 μg/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)
Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue labeling E Cadherin with ab219332 at 2 μg/mL.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] (ab219332)
Immunohistochemical analysis of formalin fixed, paraffin embedded human colon carcinoma tissue labeling E Cadherin with ab219332 at 2 μg/mL.