Mouse Monoclonal E Cadherin antibody. Carrier free. Suitable for IHC-P, ICC, WB and reacts with Human, Recombinant fragment samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDH1.
Images
![Western blot - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (AB269767), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-cdh1-1525-bsa-and-azide-free-ab269767--western-blot-img78844.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (AB269767)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (AB269767), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-cdh1-1525-bsa-and-azide-free-ab269767--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117230.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (AB269767)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (AB269767), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-cdh1-1525-bsa-and-azide-free-ab269767--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100001.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (AB269767)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (AB269767), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-cdh1-1525-bsa-and-azide-free-ab269767--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104156.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (AB269767)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (AB269767), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-cdh1-1525-bsa-and-azide-free-ab269767--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img64063.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (AB269767)")
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human CDH1. Database link P12830
Reactivity data
Select an application| IHC-P | ICC | WB | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Recombinant fragment | Not recommended | Not recommended | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Species Human | Dilution info 1.00000-2.00000 µg/mL | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
8 products for Alternative Product
Target data
Function
Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.
Alternative names
CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin
Recommended products
Mouse Monoclonal E Cadherin antibody. Carrier free. Suitable for IHC-P, ICC, WB and reacts with Human, Recombinant fragment samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CDH1.
Key facts
- Isotype
- IgG1
- Host species
- Mouse
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human CDH1. Database link P12830
- Carrier free
- Yes
- Clone number
- CDH1/1525
- Purification technique
- Affinity purification Protein G
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab269767 is a carrier free version of Anti-E Cadherin antibody [CDH1/1525] ab219332.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.
- Biological function summary
E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.
- Pathways
E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.
- Associated diseases and disorders
E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-cdh1-1525-bsa-and-azide-free-ab269767--western-blot-img78844.jpg "Western blot - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767)")
Western blot - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767)
This image was produced using Anti-E Cadherin antibody [CDH1/1525] ab219332, the same clone but in a different formulation.
All lanes: Western blot - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767) at 2 µg/mL
Lane 1: E Cadherin recombinant protein
Lane 2: Human stomach tissue lysate
Predicted band size: 97 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-cdh1-1525-bsa-and-azide-free-ab269767--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117230.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767)
Immunohistochemical analysis of formalin fixed, paraffin embedded human colon carcinoma tissue labeling E Cadherin with Anti-E Cadherin antibody [CDH1/1525] ab219332 at 2 µg/mL.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-E Cadherin antibody [CDH1/1525] ab219332).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-cdh1-1525-bsa-and-azide-free-ab269767--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img100001.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767)
Immunohistochemical analysis of formalin fixed, paraffin embedded human skin tissue labeling E Cadherin with Anti-E Cadherin antibody [CDH1/1525] ab219332 at 2 µg/mL.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-E Cadherin antibody [CDH1/1525] ab219332).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-cdh1-1525-bsa-and-azide-free-ab269767--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img104156.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767)
Immunohistochemical analysis of formalin fixed, paraffin embedded human prostate carcinoma tissue labeling E Cadherin with Anti-E Cadherin antibody [CDH1/1525] ab219332 at 2 µg/mL.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-E Cadherin antibody [CDH1/1525] ab219332).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-cdh1-1525-bsa-and-azide-free-ab269767--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img64063.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767)
Immunohistochemical analysis of formalin fixed, paraffin embedded human placenta tissue labeling E Cadherin with Anti-E Cadherin antibody [CDH1/1525] ab219332 at 2 µg/mL.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-E Cadherin antibody [CDH1/1525] ab219332).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-cdh1-1525-bsa-and-azide-free-ab269767--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103579.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767)
Immunohistochemical analysis of formalin fixed, paraffin embedded human colon carcinoma tissue labeling E Cadherin with Anti-E Cadherin antibody [CDH1/1525] ab219332 at 2 µg/mL.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-E Cadherin antibody [CDH1/1525] ab219332).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-cdh1-1525-bsa-and-azide-free-ab269767--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img103579.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [CDH1/1525] - BSA and Azide free (ab269767)")
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