AB11512

Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker

Name: Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512) | Abcam
Brand: Abcam Limited
SKU: AB11512
Rating: 4 (26 reviews)

Lab Essentials
What is this?

(26 Reviews)

(179 Publications)

Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512) is a rat monoclonal antibody detecting E Cadherin in ICC/IF. Suitable for Mouse.

- Over 140 publications
- Trusted since 2004

View Alternative Names

CD324, Cadherin-1, ARC-1, Epithelial cadherin, Uvomorulin, E-cadherin, Cdh1

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (AB11512)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (AB11512)

ab11512 staining E Cadherin - Intercellular Junction Marker in M158 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11512 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor^® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Also suitable in cells fixed with 100% methanol (5 min).

Carrier free

Anti-E Cadherin antibody [DECMA-1] - BSA and Azide free

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

DECMA-1

Isotype

IgG1

Carrier free

Reacts with

Mouse

Applications

ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Some customers have used this antibody successfully in human; however, our latest tests were unsuccessful and therefore we can no longer guarantee the reactivity of this species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512) is a rat monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.

Trusted by the scientific community
Anti-E Cadherin [DECMA-1] - Intercellular Junction Marker (ab11512) was first used in a scientific publication in 2004 and has been cited over 140 times in peer-reviewed journals.

Reviewed by scientists
Anti-E Cadherin [DECMA-1] - Intercellular Junction Marker (ab11512) has over 25 independent reviews from customers.

Other related products
We have a range of other formats of antibody clone [DECMA-1] also available for your convenience: ab11512, Carrier free - ab264106

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with Β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased Β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as Β-catenin further impacting cancer progression and pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity).. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production (By similarity).. (Microbial infection) Does not function as a receptor for L.monocytogenes internalin A (InlA); mutating a single surface-exposed residue confers receptor activity to this protein and promotes uptake of the bacteria.

See full target information Cdh1

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Publications (179)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6307 PubMed40628714

2025

Generic comparison of lumen nucleation and fusion in epithelial organoids with and without hydrostatic pressure.

Applications

Unspecified application

Species

Unspecified reactive species

Linjie Lu,Kana Fuji,Tristan Guyomar,Michèle Lieb,Marie André,Sakurako Tanida,Makiko Nonomura,Tetsuya Hiraiwa,Yara Alcheikh,Siham Yennek,Heike Petzold,Cecilie Martin-Lemaitre,Anne Grapin-Botton,Alf Honigmann,Masaki Sano,Daniel Riveline

International journal of molecular sciences 26: PubMed40243881

2025

Effects of Prebiotic Phytocompound Administration in Gestational Diabetic Dams and Its Influence on Offspring Cognitive Outcomes.

Applications

Unspecified application

Species

Unspecified reactive species

Gayathri Jagadeesan,Tushar K Das,Jennifer M Mendoza,Ghalya Alrousan,Maria P Blasco-Conesa,Parimelazhagan Thangaraj,Bhanu Priya Ganesh

eLife 12: PubMed40184163

2025

Stratification of enterochromaffin cells by single-cell expression analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Song,Linda J Fothergill,Kari S Lee,Brandon Y Liu,Ada Koo,Mark Perelis,Shanti Diwakarla,Brid Callaghan,Jie Huang,Jill Wykosky,John B Furness,Gene W Yeo

Oncology letters 29:245 PubMed40182609

2025

The feasible role of soluble E‑cadherin in spheroidogenesis of HCT116 colorectal cancer cells, a candidate biomarker for liquid biopsy.

Applications

Unspecified application

Species

Unspecified reactive species

In-Youb Chang,Hye-Jin Boo,Jin Won Hyun,Sang-Pil Yoon

International journal of ophthalmology 18:209-215 PubMed39967985

2025

Skullcapflavone II suppresses TGF-β-induced corneal epithelial mesenchymal transition .

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Xi Li,Zhen Zhang,Yue Zhang,Fan-Ru Zhao,Yu-Fan Li,Yu-Fei Dang,Yang-Yang Yue,Li Li

Scientific reports 15:4964 PubMed39929986

2025

Low-intensity pulsed ultrasound induces multifaced alterations in chromosome segregation, cytoskeletal filaments and cell junctions.

Applications

Unspecified application

Species

Unspecified reactive species

Ion Udroiu,Federica Todaro,Alessandra Vitaliti,Damiano Palmieri,Eugenia Guida,Giulia Perilli,Leonardo Duranti,Cadia D'Ottavi,Maurizio Mattei,Susanna Dolci,Gaio Paradossi,Angelico Bedini,Ida Silvestri,Antonella Sgura,Fabio Domenici

EMBO molecular medicine 16:3142-3168 PubMed39521937

2024

JAK-STAT1 as therapeutic target for EGFR deficiency-associated inflammation and scarring alopecia.

Applications

Unspecified application

Species

Unspecified reactive species

Karoline Strobl,Jörg Klufa,Regina Jin,Lena Artner-Gent,Dana Krauß,Philipp Novoszel,Johanna Strobl,Georg Stary,Igor Vujic,Johannes Griss,Martin Holcmann,Matthias Farlik,Bernhard Homey,Maria Sibilia,Thomas Bauer

BMC genomics 25:969 PubMed39415084

2024

Clusterin from endometrial glands plays a critical role in decidualization via Trem2.

Applications

Unspecified application

Species

Unspecified reactive species

Sitong Yao,Yingni Chen,Rui Cao,Lin Lu,Jingsi Yang,Wei Lei,Yugu Li,Xiaohuan Liang

Nature materials 23:1582-1591 PubMed39385019

2024

Jamming of nephron-forming niches in the developing mouse kidney creates cyclical mechanical stresses.

Applications

Unspecified application

Species

Unspecified reactive species

Louis S Prahl,Jiageng Liu,John M Viola,Aria Zheyuan Huang,Trevor J Chan,Gabriela Hayward-Lara,Catherine M Porter,Chenjun Shi,Jitao Zhang,Alex J Hughes

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2308622 PubMed39360593

2024

The T-Type Calcium Channel CACNA1H is Required for Smooth Muscle Cytoskeletal Organization During Tracheal Tubulogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ziying Liu,Chunyan Lu,Li Ma,Changjiang Li,Haiyun Luo,Yiqi Liu,Xinyuan Liu,Haiqing Li,Yachao Cui,Jiahang Zeng,Natalia Bottasso-Arias,Debora Sinner,Le Li,Jian Wang,Didier Y R Stainier,Wenguang Yin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker

Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (AB11512)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (179)

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