Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512) is a rat monoclonal antibody that is used to detect E Cadherin in ICC/IF. Suitable for Mouse samples.
- Over 140 publications
- Trusted since 2004
Images
![Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (AB11512), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-decma-1-intercellular-junction-marker-ab11512--immunocytochemistry-immunofluorescence-img110515.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (AB11512)")
Publications
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- Cells 12:2023Modeling of -Deficient Kidney Disease Using a Human Induced Pluripotent Stem Cell-Derived Kidney Organoid System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSun Woo Lim,Dohyun Na,Hanbi Lee,Xianying Fang,Sheng Cui,Yoo Jin Shin,Kang In Lee,Jae Young Lee,Chul Woo Yang,Byung Ha ChungPubMed 37759541
- Development (Cambridge, England) 150:2023Mesodermal FGF and BMP govern the sequential stages of zebrafish thyroid specification.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenoit Haerlingen,Robert Opitz,Isabelle Vandernoot,Angelo Molinaro,Meghna Parakkal Shankar,Pierre Gillotay,Achim Trubiroha,Sabine CostagliolaPubMed 37191061
- Journal of translational medicine 21:1382023Modeling of Fabry disease nephropathy using patient derived human induced pluripotent stem cells and kidney organoid system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheng Cui,Xianying Fang,Hanbi Lee,Yoo Jin Shin,Eun-Sil Koh,Sungjin Chung,Hoon Suk Park,Sun Woo Lim,Kang In Lee,Jae Young Lee,Chul Woo Yang,Byung Ha ChungPubMed 36814269
- International journal of molecular sciences 24:2023CRISPR-Cas9-Mediated Correction of Gene Mutation Rescues the Gitelman's Disease Phenotype in a Patient-Derived Kidney Organoid System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSun Woo Lim,Xianying Fang,Sheng Cui,Hanbi Lee,Yoo Jin Shin,Eun Jeong Ko,Kang In Lee,Jae Young Lee,Byung Ha Chung,Chul Woo YangPubMed 36769335
- Nature communications 13:79182022Glucose absorption drives cystogenesis in a human organoid-on-chip model of polycystic kidney disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSienna R Li,Ramila E Gulieva,Louisa Helms,Nelly M Cruz,Thomas Vincent,Hongxia Fu,Jonathan Himmelfarb,Benjamin S FreedmanPubMed 36564419
- Kidney research and clinical practice 42:75-852022Deep learning predicts the differentiation of kidney organoids derived from human induced pluripotent stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKeonhyeok Park,Jong Young Lee,Soo Young Lee,Iljoo Jeong,Seo-Yeon Park,Jin Won Kim,Sun Ah Nam,Hyung Wook Kim,Yong Kyun Kim,Seungchul LeePubMed 36328994
- International journal of molecular sciences 23:2022Molecular Mechanism of Mouse Uterine Smooth Muscle Regulation on Embryo Implantation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Cao,Zhen-Shan Yang,Sui-Li Hu,Shi-Jin Liang,Shu-Miao Zhang,Song-Qi Zhu,Lin Lu,Cheng-Hong Long,Si-Tong Yao,Yong-Jiang Ma,Xiao-Huan LiangPubMed 36293350
- Oncoimmunology 11:21272712022Tyk2 is a tumor suppressor in colorectal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesStefan Moritsch,Bernadette Mödl,Irene Scharf,Lukas Janker,Daniela Zwolanek,Gerald Timelthaler,Emilio Casanova,Maria Sibilia,Thomas Mohr,Lukas Kenner,Dietmar Herndler-Brandstetter,Christopher Gerner,Mathias Müller,Birgit Strobl,Robert EferlPubMed 36185806
- Nature biomedical engineering 6:463-4752022Modelling ciliopathy phenotypes in human tissues derived from pluripotent stem cells with genetically ablated cilia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNelly M Cruz,Raghava Reddy,José L McFaline-Figueroa,Christine Tran,Hongxia Fu,Benjamin S FreedmanPubMed 35478224
- Cellular and molecular life sciences : CMLS 79:2592022Effective high-throughput isolation of enriched platelets and circulating pro-angiogenic cells to accelerate skin-wound healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSilvia Erratico,Marzia Belicchi,Mirella Meregalli,Dario Di Silvestre,Luana Tripodi,Antonella De Palma,Rebecca Jones,Emanuele Ferrari,Laura Porretti,Elena Trombetta,Giorgio R Merlo,Pierluigi Mauri,Yvan TorrentePubMed 35474498
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| ICC/IF | |
|---|---|
| Mouse | Tested |
| Cow | Predicted |
| Dog | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
7 products for Alternative Product
Target data
Function
Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity). E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production (By similarity). (Microbial infection) Does not function as a receptor for L.monocytogenes internalin A (InlA); mutating a single surface-exposed residue confers receptor activity to this protein and promotes uptake of the bacteria.
Alternative names
CD324, Cadherin-1, ARC-1, Epithelial cadherin, Uvomorulin, E-cadherin, Cdh1
Recommended products
Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512) is a rat monoclonal antibody that is used to detect E Cadherin in ICC/IF. Suitable for Mouse samples.
- Over 140 publications
- Trusted since 2004
Key facts
- Isotype
- IgG1
- Host species
- Rat
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- DECMA-1
- Purification technique
- Affinity purification Protein G
- Specificity
Some customers have used this antibody successfully in human; however, our latest tests were unsuccessful and therefore we can no longer guarantee the reactivity of this species.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512) is a rat monoclonal antibody and is validated for use in Immunocytochemistry/immunofluorescence (ICC/IF) in Mouse samples.
Trusted by the scientific community
Anti-E Cadherin [DECMA-1] - Intercellular Junction Marker (ab11512) was first used in a scientific publication in 2004 and has been cited over 140 times in peer-reviewed journals.
Reviewed by scientists
Anti-E Cadherin [DECMA-1] - Intercellular Junction Marker (ab11512) has over 25 independent reviews from customers.
Other related products
We have a range of other formats of antibody clone [DECMA-1] also available for your convenience:
ab11512, Carrier free - Anti-E Cadherin antibody [DECMA-1] - BSA and Azide free ab264106
Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.
- Biological function summary
E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.
- Pathways
E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with Β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased Β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.
- Associated diseases and disorders
E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as Β-catenin further impacting cancer progression and pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-antibody-decma-1-intercellular-junction-marker-ab11512--immunocytochemistry-immunofluorescence-img110515.jpg "Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512)")
Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [DECMA-1] - Intercellular Junction Marker (ab11512)
ab11512 staining E Cadherin - Intercellular Junction Marker in M158 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab11512 at 5µg/ml and Anti-beta Tubulin antibody - Loading Control ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed ab150165, Goat polyclonal Secondary Antibody to Rat IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 100% methanol (5 min).
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