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Knockout Tested Rabbit Monoclonal E Cadherin antibody. Intercellular Junction marker. Suitable for mIHC, IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 750 publications.

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Shipping To:
United States

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIHC-PWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested

Images

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/50000
Notes

For unpurified protein: 1/200000 dilution

Tested
Tested

Species
Human
Dilution info
0.2-1 µg/mL
Notes

Permeabilisation is unnecessary as the immunogen is in an extracellular domain.

Tested
Tested

Species
Human
Dilution info
1/30
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For purified format use at1/1000.

Target data

Recommended products

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Knockout Tested Rabbit Monoclonal E Cadherin antibody. Intercellular Junction marker. Suitable for mIHC, IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 750 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Clone number

EP700Y

Purification technique

Affinity purification Protein A

Specificity

E-cadherin contains a number of cleavage sites which may yield a complex fragmentation pattern in WB. Multiple bands between ~80-120 kDa may be observed. This antibody has been tested on human samples in both WB and IHC. Customer feedback (see Abreview) suggests the antibody does not perform well in IHC on mouse tissue.

Dissociation constant

2.8 x 1011 M

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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