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Knockout Tested Rabbit Monoclonal E Cadherin antibody. Intercellular Junction marker. Suitable for mIHC, IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 750 publications.
Alternative names=Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin, CDHE, UVO, CDH1
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Liquid
Monoclonal
The exact immunogen used to generate this antibody is proprietary information.
mIHC | IHC-P | WB | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|---|
Human | Tested | Tested | Tested | Tested | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/1000 - 1/50000 | Notes For unpurified protein: 1/200000 dilution |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 0.2-1 µg/mL | Notes Permeabilisation is unnecessary as the immunogen is in an extracellular domain. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/30 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. For purified format use at1/1000. |
Knockout Tested Rabbit Monoclonal E Cadherin antibody. Intercellular Junction marker. Suitable for mIHC, IHC-P, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 750 publications.
Alternative names=Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin, CDHE, UVO, CDH1
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Liquid
Monoclonal
The exact immunogen used to generate this antibody is proprietary information.
EP700Y
Affinity purification Protein A
E-cadherin contains a number of cleavage sites which may yield a complex fragmentation pattern in WB. Multiple bands between ~80-120 kDa may be observed. This antibody has been tested on human samples in both WB and IHC. Customer feedback (see Abreview) suggests the antibody does not perform well in IHC on mouse tissue.
2.8 x 1011 M
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Abcam recommended secondaries - Goat Anti-Rabbit HRP (ab205718) and Goat Anti-Rabbit Alexa Fluor® 488 (ab150077). Or search our wide range of secondary antibodies for use with your experiment.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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