AB181296

Anti-E Cadherin antibody [EPR16845-108]

RabMAb
Recombinant
Lab Essentials
20ul selling size
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(6 Publications)

Rabbit Recombinant Monoclonal E Cadherin antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

CD324, Cadherin-1, ARC-1, Epithelial cadherin, Uvomorulin, E-cadherin, Cdh1

4 Images

Supplier Data

Western blot - Anti-E Cadherin antibody [EPR16845-108] (AB181296)

Exposure time : Lanes 1-2 : 30 seconds; Lanes 3-4 : 15 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile observed is consistent with what has been described in the literature (PMID : 11076937; PMID : 11953314).

All lanes:

Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296) at 1/1000 dilution

Lane 1:

Rat spleen lysate at 10 µg

Lane 2:

Mouse plasma at 10 µg

Lane 3:

Mouse serum at 10 µg

Lane 4:

Mouse brain lysate at 10 µg

Secondary

Lanes 1 and 4:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 2 - 3:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 97 kDa

Observed band size: 120 kDa,84 kDa

true

Supplier Data

Western blot - Anti-E Cadherin antibody [EPR16845-108] (AB181296)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 28220208).

Low expression : spleen, skeletal muscle (PMID : 7734012).

The identity of the bands at 37 kDa and 60 kDa are unknown.

Samples are non-boiled as boiling may cause protein aggregation.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-E Cadherin antibody [EPR16845-108] (ab181296) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-ABCB4 antibody [EPR28774-92] (<a href='/en-us/products/primary-antibodies/abcb4-antibody-epr28774-92-ab317573'>ab317573</a>) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate non-membrane fraction at 20 µg with NFDM/TBST

Lane 2:

Mouse liver tissue lysate membrane fraction at 20 µg with NFDM/TBST

Lane 3:

Mouse liver tissue lysate at 20 µg with NFDM/TBST

Lane 4:

Mouse spleen tissue lysate at 20 µg with NFDM/TBST

Lane 5:

Mouse skeletal muscle tissue lysate at 20 µg with NFDM/TBST

Lane 6:

Rat liver tissue lysate at 20 µg with NFDM/TBST

Lane 7:

Rat spleen tissue lysate at 20 µg with NFDM/TBST

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 140-150 kDa,36 kDa,125 kDa

true

Exposure time: 180s

Supplier Data

Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-108] (AB181296)

E Cadherin was immunoprecipitated from 1 mg of mouse serum with ab181296 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181296 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse serum 10 μg (Input).

Lane 2 : ab181296 IP in mouse serum.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab181296 in mouse serum.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

All lanes:

Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-108] (ab181296)

Predicted band size: 97 kDa

false

Supplier Data

Western blot - Anti-E Cadherin antibody [EPR16845-108] (AB181296)

Lane 1:

Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296) at 1/20000 dilution

Lane 2:

Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296) at 1/100000 dilution

All lanes:

His-tagged mouse E-Cadherin active protein (aa1-709) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 32 kDa,97 kDa

Observed band size: 120 kDa,84 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16845-108

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity).. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production (By similarity).. (Microbial infection) Does not function as a receptor for L.monocytogenes internalin A (InlA); mutating a single surface-exposed residue confers receptor activity to this protein and promotes uptake of the bacteria.

See full target information Cdh1

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 27:456-469 PubMed36651490

2023

Pirfenidone suppressed triple-negative breast cancer metastasis by inhibiting the activity of the TGF-β/SMAD pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Daiqin Luo,Xianlin Zeng,Shuling Zhang,Daohong Li,Zhimei Cheng,Yun Wang,Jinhua Long,Zuquan Hu,Shiqi Long,Jing Zhou,Shuai Zhang,Zhu Zeng

Cancer cell international 22:221 PubMed35773697

2022

VEGF-B targeting by aryl hydrocarbon receptor mediates the migration and invasion of choriocarcinoma stem-like cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qianxia Tan,Jingting Cai,Jingping Peng,Cui Hu,ChenChun Wu,Huining Liu

Frontiers in physiology 13:778479 PubMed35309056

2022

Inhibition of PI3K/AKT/mTOR Signalling Pathway Activates Autophagy and Suppresses Peritoneal Fibrosis in the Process of Peritoneal Dialysis.

Applications

Unspecified application

Species

Unspecified reactive species

Miao Jia,Hong Qiu,Lihua Lin,Shun Zhang,Damei Li,Donghua Jin

Molecular medicine reports 25: PubMed34841435

2021

MicroRNA‑671‑5p inhibits cell proliferation, migration and invasion in non‑small cell lung cancer by targeting MFAP3L.

Applications

Unspecified application

Species

Unspecified reactive species

Junhui Ye,Wujun Luo,Lingling Luo,Limin Zhai,Pingping Huang

Experimental and therapeutic medicine 22:1102 PubMed34504556

2021

Sema4C modulates the migration of primary tumor-associated lymphatic endothelial cells via an ERK-mediated pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Peng,Xijiang Liu,Chengcheng Li,Min Gao,Hongyan Wang

Gut microbes 12:1788891 PubMed32686598

2020

Gut microbiota regulate tumor metastasis via circRNA/miRNA networks.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuxian Zhu,Jianguo Huang,Xu Li,Jun Xing,Qiang Chen,Ruilin Liu,Feng Hua,Zhongmin Qiu,Yuanlin Song,Chunxue Bai,Yin-Yuan Mo,Ziqiang Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-E Cadherin antibody [EPR16845-108]

Western blot - Anti-E Cadherin antibody [EPR16845-108] (AB181296)

Western blot - Anti-E Cadherin antibody [EPR16845-108] (AB181296)

Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-108] (AB181296)

Western blot - Anti-E Cadherin antibody [EPR16845-108] (AB181296)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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