Skip to main content

Rabbit Recombinant Monoclonal E Cadherin antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 6 publications.

Be the first to review this product! Submit a review

Images

Western blot - Anti-E Cadherin antibody [EPR16845-108] (AB181296), expandable thumbnail
  • Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-108] (AB181296), expandable thumbnail
  • Western blot - Anti-E Cadherin antibody [EPR16845-108] (AB181296), expandable thumbnail
  • Western blot - Anti-E Cadherin antibody [EPR16845-108] (AB181296), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Mouse
Tested
Tested
Rat
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity). E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production (By similarity). (Microbial infection) Does not function as a receptor for L.monocytogenes internalin A (InlA); mutating a single surface-exposed residue confers receptor activity to this protein and promotes uptake of the bacteria.

Alternative names

CD324, Cadherin-1, ARC-1, Epithelial cadherin, Uvomorulin, E-cadherin, Cdh1

Recommended products

Rabbit Recombinant Monoclonal E Cadherin antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 6 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR16845-108
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

Associated diseases and disorders

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296), expandable thumbnail

    Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296)

    Lane 1: Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296) at 1/20000 dilution

    Lane 2: Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296) at 1/100000 dilution

    All lanes: His-tagged mouse E-Cadherin active protein (aa1-709) at 0.01 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 32 kDa, 97 kDa

    Observed band size: 120 kDa, 84 kDa

    Exposure time: 10s

  • Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-108] (ab181296), expandable thumbnail

    Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-108] (ab181296)

    E Cadherin was immunoprecipitated from 1 mg of mouse serum with ab181296 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181296 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse serum 10 μg (Input).

    Lane 2: ab181296 IP in mouse serum.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab181296 in mouse serum.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 1 second.

    All lanes: Immunoprecipitation - Anti-E Cadherin antibody [EPR16845-108] (ab181296)

    Predicted band size: 97 kDa

  • Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296), expandable thumbnail

    Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296)

    Exposure time : Lanes 1-2: 30 seconds; Lanes 3-4: 15 seconds.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The expression profile observed is consistent with what has been described in the literature (PMID: 11076937; PMID: 11953314).

    All lanes: Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296) at 1/1000 dilution

    Lane 1: Rat spleen lysate at 10 µg

    Lane 2: Mouse plasma at 10 µg

    Lane 3: Mouse serum at 10 µg

    Lane 4: Mouse brain lysate at 10 µg

    Secondary

    Lanes 1 and 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Lanes 2 - 3: Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 97 kDa

    Observed band size: 120 kDa, 84 kDa

  • Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296), expandable thumbnail

    Western blot - Anti-E Cadherin antibody [EPR16845-108] (ab181296)

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28220208).

    Low expression: spleen, skeletal muscle (PMID: 7734012).

    The identity of the bands at 37 kDa and 60 kDa are unknown.

    Samples are non-boiled as boiling may cause protein aggregation.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    In Western blot, Anti-E Cadherin antibody [EPR16845-108] (ab181296) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-ABCB4 antibody [EPR28774-92] (Anti-ABCB4 antibody [EPR28774-92] ab317573) at 1/1000 dilution

    Lane 1: Mouse liver tissue lysate non-membrane fraction at 20 µg with NFDM/TBST

    Lane 2: Mouse liver tissue lysate membrane fraction at 20 µg with NFDM/TBST

    Lane 3: Mouse liver tissue lysate at 20 µg with NFDM/TBST

    Lane 4: Mouse spleen tissue lysate at 20 µg with NFDM/TBST

    Lane 5: Mouse skeletal muscle tissue lysate at 20 µg with NFDM/TBST

    Lane 6: Rat liver tissue lysate at 20 µg with NFDM/TBST

    Lane 7: Rat spleen tissue lysate at 20 µg with NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 140-150 kDa, 36 kDa, 125 kDa

    Exposure time: 180s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com