Anti-E Cadherin antibody [EPR30045-525]

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with a mouse CDH1 expression vector containing a myc-His-tag® cells labelling E Cadherin with ab324191 at 1/100 (5.22 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing positive staining in 293T cell line transfected with a mouse CDH1 expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse breast tissue staining PNPLA3 with ab324436 at a 1/200 dilution, ab324191 anti-E Cadherin used at 1/500 dilution and ab180862 anti-SOX10 used at a 1/4000 dilution.

Panel A : anti-PNPLA3 (green; Opal™520), anti-E Cadherin (magenta; Opal™690) and anti-SOX10 (gray; Opal™570) on mouse breast.

Panel B : anti-PNPLA3 staining white adipose tissue in mouse breast.

Panel C : anti-E Cadherin staining membrane of epithelium in mouse breast.

Panel D : anti-SOX10 staining nucleus of epithelium in mouse breast.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324436, ab324191 and ab180862 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse breast tissue staining Heparan Sulfate Proteoglycan 2/Perlecan with ab315029 at a 1/100 dilution, ab324191 anti-E Cadherin used at 1/500 dilution and ab316115 anti-Perilipin-1 used at a 1/2000 dilution.

Panel A : merged staining of anti-Perlecan (green; Opal™520), anti-E Cadherin (magenta; Opal™690) and anti-Perilipin-1 (gray; Opal™570) on mouse breast.

Panel B : anti-Perlecan staining endothelium and basement membrane in mouse breast.

Panel C : anti-E Cadherin staining epithelium in mouse breast.

Panel D : anti-Perilipin-1 staining adipocytes in mouse breast.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325029, ab324191 and ab316115 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling E Cadherin with ab324191 at 1/500 (2.088 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse cerebrum. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• mIHC

Lab

Multiplex immunohistochemistry - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections Mouse Prostate tissue labelling E Cadherin with ab324191 at 1 : 500 dilution (B), Cytokeratin 5 with ab236216 at 1 : 1500 dilution (C) and Collagen VI with ab271938 at 1 : 2000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-E Cadherin (green; Opal™690), anti-Cytokeratin 5 (magenta; Opal™520) and anti-Collagen VI (gray; Opal™570) on mouse prostate.

Panel B : anti-E Cadherin staining epithelium in mouse prostate.

Panel C : ant-Cytokeratin 5 staining basal cells in mouse prostate.

Panel D : ant-Collagen VI staining stoma in mouse prostate.

Nuclear DNA was labelled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324191, ab236216 and ab271938 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Immunohistochemistry analysis of Formalin/PFA-fixed paraffin-embedded sections Mouse Prostate tissue labelling E Cadherin with ab324191 at 1 : 500 dilution (B), Cytokeratin 5 with ab236216 at 1 : 1500 dilution (C) and smooth muscle Myosin heavy chain 11 with ab240983 at 1 : 4000 dilution (D). Opal Polymer HRP Ms + Rb was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Panel A : merged staining of anti-E Cadherin (green; Opal™690), anti-Cytokeratin 5 (magenta; Opal™520) and anti-smooth muscle Myosin heavy chain 11 (gray; Opal™570) on mouse prostate.

Panel B : anti-E Cadherin staining epithelium in mouse prostate.

Panel C : ant-Cytokeratin 5 staining basal cells in mouse prostate.

Panel D : ant-smooth muscle Myosin heavy chain 11 staining smooth muscle in mouse prostate.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324191, ab236216 and ab240983 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse breast tissue staining CD34 with ab316277 at a 1 : 10000 (0.05 ug/ml) dilution, ab324191 E Cadherin used at 1 : 500 (0.228 ug/ml) dilution and ab316115 Perilipin-1 used at 1 : 2000 (0.251 μg/ml) dilution.

Panel A : merged staining of anti-CD34 (green; Opal™570), anti-E Cadherin (magenta; Opal™690) and anti-Perilipin-1 (gray; Opal™570) on mouse breast.

Panel B : anti-CD34 staining endothelium in mouse breast.

Panel C : ant-E Cadherin staining epithelium in mouse breast.

Panel D : ant-Perilipin-1 staining adipocytes in mouse breast.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab316277, ab324191 and ab316115 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling E Cadherin with ab324191 at 1/500 (2.088 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : weak staining on mouse testis. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling E Cadherin with ab324191 at 1/500 (2.088 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse colon (PMID : 35371360). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HC11 (mouse mammary gland epithelial cell) cells labelling E Cadherin with ab324191 at 1/100 (5.22 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing membranous staining in HC11 cell line and no staining in EL4 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Negative control : EL4.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab324191 at 1/100 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution. -ve control 2 : ab7291 at 1/1000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling E Cadherin with ab324191 at 1/500 (2.088 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse breast cancer. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon (fresh frozen) tissue labeling E Cadherin with ab324191 at 1/100 (5.22 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Confocal image showing positive staining on mouse colon. The nuclear counterstain was DAPI (Blue). The section was incubated with the primary antibody for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse testis (fresh frozen) tissue labeling E Cadherin with ab324191 at 1/100 (5.22 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Low expression : confocal image showing positive staining on mouse testis. The nuclear counterstain was DAPI (Blue). The section was incubated with the primary antibody for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse breast tissue staining Hormone sensitive lipase/HSL with ab325336 at a 1/2000 (0.248 μg/ml) dilution, ab324191 anti-E Cadherin used at a 1/500 (2.088 μg/ml) dilution and ab180862 anti-SOX10 used at a 1/4000 (0.03 μg/ml) dilution.

Panel A : anti-Hormone sensitive lipase/HSL (green; Opal™520), anti-E Cadherin (magenta; Opal™690), anti-SOX10 (gray; Opal™570) on mouse breast.

Panel B : anti-Hormone sensitive lipase/HSL staining adipocytes in mouse breast.

Panel C : anti-E Cadherin staining epithelium in mouse breast.

Panel D : anti-SOX10 staining on nucleus in mouse breast.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab325336, ab324191 and ab180862 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] (AB324191)

Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling E Cadherin with ab324191 at 1/500 (2.088 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse stomach. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins