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Rabbit Recombinant Monoclonal E Cadherin antibody. Carrier free. Suitable for IHC-Fr, ICC/IF, IHC-P and reacts with Mouse, Transfected cell line - Mouse samples.

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Images

Immunohistochemistry (Frozen sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (AB324198), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (AB324198), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (AB324198), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (AB324198), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (AB324198), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-FrICC/IFIHC-P
Mouse
Tested
Tested
Tested
Transfected cell line - Mouse
Not recommended
Tested
Not recommended

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Transfected cell line - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Transfected cell line - Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell line - Mouse
Dilution info
-
Notes

-

Target data

Function

Cadherins are calcium-dependent cell adhesion proteins (PubMed:11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed:11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7 (By similarity). E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production (By similarity). (Microbial infection) Does not function as a receptor for L.monocytogenes internalin A (InlA); mutating a single surface-exposed residue confers receptor activity to this protein and promotes uptake of the bacteria.

Alternative names

CD324, Cadherin-1, ARC-1, Epithelial cadherin, Uvomorulin, E-cadherin, Cdh1

Rabbit Recombinant Monoclonal E Cadherin antibody. Carrier free. Suitable for IHC-Fr, ICC/IF, IHC-P and reacts with Mouse, Transfected cell line - Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR30045-525
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab324198 is the carrier-free version of Anti-E Cadherin antibody [EPR30045-525] ab324191

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Frozen sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198)

    E Cadherin Immunohistochemistry (Frozen sections) staining of Mouse testis (fresh frozen) using rabbit Anti-E Cadherin antibody

    This data was developed using Anti-E Cadherin antibody [EPR30045-525] ab324191, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse testis (fresh frozen) tissue labeling E Cadherin with Anti-E Cadherin antibody [EPR30045-525] ab324191 at 1/100 (5.22 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Low expression: confocal image showing positive staining on mouse testis. The nuclear counterstain was DAPI (Blue). The section was incubated with the primary antibody for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198)

    E Cadherin Immunocytochemistry/ Immunofluorescence staining using rabbit Anti-E Cadherin antibody

    This data was developed using Anti-E Cadherin antibody [EPR30045-525] ab324191, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HC11 (mouse mammary gland epithelial cell) cells labelling E Cadherin with Anti-E Cadherin antibody [EPR30045-525] ab324191 at 1/100 (5.22 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

    Confocal image showing membranous staining in HC11 cell line and no staining in EL4 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Negative control: EL4.

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    -ve control 1: Anti-E Cadherin antibody [EPR30045-525] ab324191 at 1/100 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) antibody at 1/1000 dilution.
    -ve control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198)

    E Cadherin Immunocytochemistry/ Immunofluorescence staining of 293T (Human embryonic kidney epithelial cell) transfected with a mouse CDH1 expression vector containing a myc-His-tag® using rabbit Anti-E Cadherin antibody

    This data was developed using Anti-E Cadherin antibody [EPR30045-525] ab324191, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with a mouse CDH1 expression vector containing a myc-His-tag® cells labelling E Cadherin with Anti-E Cadherin antibody [EPR30045-525] ab324191 at 1/100 (5.22 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

    Confocal image showing positive staining in 293T cell line transfected with a mouse CDH1 expression vector containing a myc-His-tag® (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Myc-Tag Mouse mAb (Alexa Fluor® 647) was used to counterstain tubulin at 1/100 dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198)

    E Cadherin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse colon using rabbit Anti-E Cadherin antibody

    This data was developed using Anti-E Cadherin antibody [EPR30045-525] ab324191, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling E Cadherin with Anti-E Cadherin antibody [EPR30045-525] ab324191 at 1/500 (2.088 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse colon (PMID: 35371360). The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198)

    E Cadherin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse stomach using rabbit Anti-E Cadherin antibody

    This data was developed using Anti-E Cadherin antibody [EPR30045-525] ab324191, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling E Cadherin with Anti-E Cadherin antibody [EPR30045-525] ab324191 at 1/500 (2.088 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse stomach. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198)

    E Cadherin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse breast cancer using rabbit Anti-E Cadherin antibody

    This data was developed using Anti-E Cadherin antibody [EPR30045-525] ab324191, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse breast cancer tissue labeling E Cadherin with Anti-E Cadherin antibody [EPR30045-525] ab324191 at 1/500 (2.088 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Positive staining on mouse breast cancer. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198)

    E Cadherin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse testis using rabbit Anti-E Cadherin antibody

    This data was developed using Anti-E Cadherin antibody [EPR30045-525] ab324191, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling E Cadherin with Anti-E Cadherin antibody [EPR30045-525] ab324191 at 1/500 (2.088 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Low expression: weak staining on mouse testis. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198)

    E Cadherin Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) staining of Mouse cerebrum using rabbit Anti-E Cadherin antibody

    This data was developed using Anti-E Cadherin antibody [EPR30045-525] ab324191, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling E Cadherin with Anti-E Cadherin antibody [EPR30045-525] ab324191 at 1/500 (2.088 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Negative control: no staining on mouse cerebrum. The primary antibody was incubated for 30 mins at room temperature.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument

    Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Frozen sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-E Cadherin antibody [EPR30045-525] - BSA and Azide free (ab324198)

    E Cadherin Immunohistochemistry (Frozen sections) staining of Mouse colon (fresh frozen) using rabbit Anti-E Cadherin antibody

    This data was developed using Anti-E Cadherin antibody [EPR30045-525] ab324191, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse colon (fresh frozen) tissue labeling E Cadherin with Anti-E Cadherin antibody [EPR30045-525] ab324191 at 1/100 (5.22 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

    Confocal image showing positive staining on mouse colon. The nuclear counterstain was DAPI (Blue). The section was incubated with the primary antibody for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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