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Rabbit Recombinant Monoclonal E Cadherin antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Rat, Cat, Human, Dog samples. Immunogen corresponding to Recombinant Fragment Protein within Human CDH1.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HL1229] (AB308347), expandable thumbnail
  • Western blot - Anti-E Cadherin antibody [HL1229] (AB308347), expandable thumbnail
  • Western blot - Anti-E Cadherin antibody [HL1229] (AB308347), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HL1229] (AB308347), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (AB308347), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human CDH1. The exact immunogen used to generate this antibody is proprietary information. Database link P12830

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PICC/IFWB
Human
Expected
Tested
Tested
Mouse
Tested
Expected
Tested
Rat
Tested
Expected
Expected
Cat
Tested
Expected
Expected
Dog
Expected
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Cat
Dilution info
1/100
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Human, Dog
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Mouse, Rat, Cat, Dog
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Dog
Dilution info
1/1000
Notes

-

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Rat, Cat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

CDH1 encodes a protein that is a calcium-dependent cell adhesion molecule, playing a role in cell-cell adhesion, mobility, and proliferation in epithelial cells. It acts as a potent invasive suppressor and serves as a ligand for integrin alpha-E/beta-7. The protein contributes to the sorting of heterogeneous cell types due to its homophilic interactions. E-Cad/CTF2, a product of CDH1, promotes the non-amyloidogenic degradation of Abeta precursors and strongly inhibits the production of APP C99 and C83. Additionally, in the context of microbial infection, it functions as a receptor for Listeria monocytogenes, with internalin A binding to it to promote bacterial uptake. This supplementary information is collated from multiple sources and compiled automatically.

Alternative names

CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin

Recommended products

Rabbit Recombinant Monoclonal E Cadherin antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Rat, Cat, Human, Dog samples. Immunogen corresponding to Recombinant Fragment Protein within Human CDH1.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human CDH1. The exact immunogen used to generate this antibody is proprietary information. Database link P12830
Carrier free
Yes
Clone number
HL1229
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

Associated diseases and disorders

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HL1229] (ab308347), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HL1229] (ab308347)

    Immunohistochemical analysis of paraffin-embedded cat pancreas tissue labelling E-Cadherin with ab308347 at 1/100 dilution.

    Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • Western blot - Anti-E Cadherin antibody [HL1229] (ab308347), expandable thumbnail

    Western blot - Anti-E Cadherin antibody [HL1229] (ab308347)

    Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (ab308347) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-E Cadherin antibody [HL1229] (ab308347) at 1/1000 dilution

    Lane 1: MCF7 cell lysate at 30 µg

    Lane 2: MDA-MD-231 cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 97.5 kDa

  • Western blot - Anti-E Cadherin antibody [HL1229] (ab308347), expandable thumbnail

    Western blot - Anti-E Cadherin antibody [HL1229] (ab308347)

    Separated by 5% SDS-PAGE.

    All lanes: Western blot - Anti-E Cadherin antibody [HL1229] (ab308347) at 1/1000 dilution

    All lanes: MDCK cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 97.5 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HL1229] (ab308347), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HL1229] (ab308347)

    For immunofluorescence analysis, MCF-7 cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of E-Cadherin using ab308347 at 1/500 dilution (green). Blue: Fluoroshield with DAPI

  • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (ab308347), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (ab308347)

    Immunohistochemical analysis of rat colon tissue labeling E Cadherin using ab308347 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

  • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (ab308347), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (ab308347)

    Immunohistochemical analysis of mouse intestine tissue labeling E Cadherin using ab308347 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

  • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (ab308347), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (ab308347)

    Immunohistochemical analysis of mouse stomach tissue labeling E Cadherin using ab308347 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

  • Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (ab308347), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (ab308347)

    Immunohistochemical analysis of mouse colon tissue labeling E Cadherin using ab308347 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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