AB308347

Anti-E Cadherin antibody [HL1229]

Recombinant
What is this?

Be the first to review this product! Submit a review

(1 Publication)

Rabbit Recombinant Monoclonal E Cadherin antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Mouse, Rat, Cat, Human, Dog samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CDH1.

View Alternative Names

CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin

9 Images

Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HL1229] (AB308347)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HL1229] (AB308347)

For immunofluorescence analysis, MCF-7 cells were fixed in 4% paraformaldehyde at room temperature for 15 minutes and permeabilized for detection of E-Cadherin using ab308347 at 1/500 dilution (green). Blue : Fluoroshield with DAPI

Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (AB308347)

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (AB308347)

Immunohistochemical analysis of mouse intestine tissue labeling E Cadherin using ab308347 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (AB308347)

Immunohistochemical analysis of mouse colon tissue labeling E Cadherin using ab308347 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (AB308347)

Immunohistochemical analysis of rat colon tissue labeling E Cadherin using ab308347 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (AB308347)

Immunohistochemical analysis of mouse stomach tissue labeling E Cadherin using ab308347 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-E Cadherin antibody [HL1229] (AB308347)

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody [HL1229] (ab308347) diluted at 1/1000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-E Cadherin antibody [HL1229] (ab308347) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 30 µg

Lane 2:

MDA-MD-231 cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 97.5 kDa

false

Supplier Data

Western blot - Anti-E Cadherin antibody [HL1229] (AB308347)

Separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-E Cadherin antibody [HL1229] (ab308347)

All lanes:

Mouse intestine tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 97 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HL1229] (AB308347)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HL1229] (AB308347)

Immunohistochemical analysis of paraffin-embedded cat pancreas tissue labelling E-Cadherin with ab308347 at 1/100 dilution.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-E Cadherin antibody [HL1229] (AB308347)

Separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-E Cadherin antibody [HL1229] (ab308347) at 1/1000 dilution

All lanes:

MDCK cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 97.5 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1229

Isotype

IgG

Carrier free

Yes

Reacts with

Dog, Human, Mouse, Rat, Cat

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human CDH1. The exact immunogen used to generate this antibody is proprietary information.

P12830

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

style

left-column

Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.

Biological function summary

E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.

Pathways

E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.

E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Cadherins are calcium-dependent cell adhesion proteins (PubMed : 11976333). They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH1 is involved in mechanisms regulating cell-cell adhesions, mobility and proliferation of epithelial cells (PubMed : 11976333). Promotes organization of radial actin fiber structure and cellular response to contractile forces, via its interaction with AMOTL2 which facilitates anchoring of radial actin fibers to CDH1 junction complexes at the cell membrane (By similarity). Plays a role in the early stages of desmosome cell-cell junction formation via facilitating the recruitment of DSG2 and DSP to desmosome plaques (PubMed : 29999492). Has a potent invasive suppressor role. It is a ligand for integrin alpha-E/beta-7.. E-Cad/CTF2 promotes non-amyloidogenic degradation of Abeta precursors. Has a strong inhibitory effect on APP C99 and C83 production.. (Microbial infection) Serves as a receptor for Listeria monocytogenes; internalin A (InlA) binds to this protein and promotes uptake of the bacteria.

See full target information CDH1

style

left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

BMC complementary medicine and therapies 24:258 PubMed38987744

2024

Pasteurized form of a potential probiotic lactobacillus brevis IBRC-M10790 exerts anti-inflammatory effects on inflammatory bowel disease in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Ardeshir Ebrahiminejad,Abbas Akhavan Sepahi,Abbas Yadegar,Anna Meyfour

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-E Cadherin antibody [HL1229]

Immunocytochemistry/ Immunofluorescence - Anti-E Cadherin antibody [HL1229] (AB308347)

Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (AB308347)

Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (AB308347)

Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (AB308347)

Immunohistochemistry paraffin embedded sections - Anti-E Cadherin antibody [HL1229] (AB308347)

Western blot - Anti-E Cadherin antibody [HL1229] (AB308347)

Western blot - Anti-E Cadherin antibody [HL1229] (AB308347)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [HL1229] (AB308347)

Western blot - Anti-E Cadherin antibody [HL1229] (AB308347)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise