Anti-E Cadherin antibody [SP64] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [SP64] - BSA and Azide free (AB240984)

Formalin-fixed, paraffin-embedded human prostate tissue stained for E Cadherin using ab227639 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227639)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [SP64] - BSA and Azide free (AB240984)

Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for E Cadherin using ab227639 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227639)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [SP64] - BSA and Azide free (AB240984)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast carcinoma tissue sections labeling E Cadherin with ab227639 at 1/100 dilution (1.22 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human breast carcinoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227639 for 10 mins at room temperature.

This image was generated using ab227639, the same clone, but with a different buffer formulation.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [SP64] - BSA and Azide free (AB240984)

Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for E Cadherin using ab227639 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227639)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [SP64] - BSA and Azide free (AB240984)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human skin tissue sections labeling E Cadherin with ab227639 at 1/100 dilution (1.22 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human skin, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227639 for 10 mins at room temperature.

This image was generated using ab227639, the same clone, but with a different buffer formulation.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [SP64] - BSA and Azide free (AB240984)

Formalin-fixed, paraffin-embedded human skin tissue stained for E Cadherin using ab227639 at 1/100 dilution in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab227639)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin antibody [SP64] - BSA and Azide free (AB240984)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling E Cadherin with ab227639 at 1/100 dilution (1.22 μg/ml). Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human liver, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227639 for 10 mins at room temperature.

This image was generated using ab227639, the same clone, but with a different buffer formulation.

• WB

Lab

Western blot - Anti-E Cadherin antibody [SP64] - BSA and Azide free (AB240984)

This image was generated using ab227639, the same clone, but with a different buffer formulation. False colour image of Western blot : Anti-E Cadherin antibody [SP64] staining at 1/100 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab227639 was shown to bind specifically to E Cadherin. A band was observed at 105/130 kDa in wild-type A431 cell lysates with no signal observed at this size in CDH1 knockout cell line ab273747 (knockout cell lysate ab273781). To generate this image, wild-type and CDH1 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

Lanes 1 - 4:

Western blot - Anti-E Cadherin antibody [SP64] (<a href='/en-us/products/primary-antibodies/e-cadherin-antibody-sp64-ab227639'>ab227639</a>) at 1/100 dilution

Lanes 1 - 4:

Western blot - Anti-E Cadherin antibody [SP64] - BSA and Azide free (ab240984) at 1/100 dilution

Lane 1:

Wild-type A431 cell lysate at 20 µg

Lane 2:

Western blot - Human CDH1 (E Cadherin) knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-cdh1-e-cadherin-knockout-a-431-cell-line-ab273747'>ab273747</a>)

Lane 2:

Western blot - Human CDH1 (E Cadherin) knockout A-431 cell lysate (ab273781)

Lane 2:

CDH1 knockout A431 cell lysate at 20 µg

Lane 3:

MCF7 cell lysate at 20 µg

Lane 4:

MDA-MB-231 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 105 kDa,130 kDa

false