Rabbit Recombinant Monoclonal E Cadherin phospho S838 + S840 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 62 publications.
Images
![Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-phospho-s838-s840-antibody-ep9132y-ab76319--western-blot-img144798.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-phospho-s838-s840-antibody-ep9132y-ab76319--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118854.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)")
![Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-phospho-s838-s840-antibody-ep9132y-ab76319--western-blot-img44022.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-phospho-s838-s840-antibody-ep9132y-ab76319--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124344.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-phospho-s838-s840-antibody-ep9132y-ab76319--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111535.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (AB76319)")
Publications
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- BMC cancer 23:10212023Identification of a basement membrane-related genes signature with immune correlation in bladder urothelial carcinoma and verification in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanze Li,Kai Xu,Ye Zhang,Hu Mao,Qiangmin Qiu,Zhiwei Yan,Xiuheng Liu,Yang Du,Zhiyuan ChenPubMed 37872487
- American journal of translational research 15:5047-50702023Matrine improves the hepatic microenvironment and reverses epithelial-mesenchymal transition in hepatocellular carcinoma by inhibiting the Wnt-1/β-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianfei Shi,Hua Zhang,Guangjie Han,Jinfeng Wang,Xin Han,Man Zhao,Xiaoling Duan,Lili Mi,Ning Li,Xiaolei Yin,Cuizhen Li,Fei YinPubMed 37692966
- International journal of molecular sciences 24:2023TRIM14 Overexpression Induces Chemoresistance and Malignant Behaviors of Hepatocellular Carcinoma Cells by Activating the STAT3/HIF-1α Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiqi Xu,Lihong Zhuang,Hongxu Zhu,Anrong Mao,Jiamin Zhou,Lu WangPubMed 37628777
- BMC biology 21:1012023Insights into the formation and diversification of a novel chiropteran wing membrane from embryonic development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeal Anthwal,Daniel J Urban,Alexa Sadier,Risa Takenaka,Simon Spiro,Nancy Simmons,Richard R Behringer,Chris J Cretekos,John J Rasweiler,Karen E SearsPubMed 37143038
- Genome integrity 14:22023Therapeutic Fractional Doses of Ionizing Radiation Promote Epithelial-Mesenchymal Transition, Enhanced Invasiveness, and Altered Glycosylation in MCF-7 Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRaheem Al-Abedi,Seda Tuncay Cagatay,Ammar Mayah,Susan A Brooks,Munira KadhimPubMed 38025522
- International journal of molecular sciences 24:2023Inhibition of Ribosomal RNA Processing 15 Homolog (RRP15) Suppressed Tumor Growth, Invasion and Epithelial to Mesenchymal Transition (EMT) of Colon Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZirong Deng,Yun Xu,Yuchen Cai,Weiling Lin,Libei Zhang,Aoqing Jiang,Yuhang Zhou,Rui Zhao,Heyan Zhao,Zhaoguo Liu,Tingdong YanPubMed 36834940
- Journal of neuroimmunology 371:5779292022Activin A is a novel chemoattractant for migration of microglial BV2 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYunfeng Wang,Yan Qi,Jianfei Qi,Jiandong Wu,Francis Lin,Xueling Cui,Jingyan Ge,Zhonghui LiuPubMed 35939944
- Journal of oncology 2022:83269402022MACC1 Promotes the Progression and Is a Novel Biomarker for Predicting Immunotherapy Response in Colorectal Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMan Xiong,Mingsen Wang,Youhong Yan,Xiaowu Chen,Wanwei Guo,Ming Xu,Shaoyan Guo,Yeyang WangPubMed 35874635
- Stem cells international 2022:19407612022Extracellular Vesicles Derived from Adipose Mesenchymal Stem Cells Promote Peritoneal Healing by Activating MAPK-ERK1/2 and PI3K-Akt to Alleviate Postoperative Abdominal Adhesion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManyu Shi,Hengchen Liu,Tingting Zhang,Mingzhao Zhang,Xin Tang,Zenan Zhang,Wenjun Lu,Shulong Yang,Zhitao Jiang,Qingbo Cui,Zhaozhu LiPubMed 35578661
- Aging 14:2945-29652022E-cadherin deficiency promotes prostate macrophage inflammation and bladder overactivity in aged male mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLaura E Pascal,Taro Igarashi,Shinsuke Mizoguchi,Wei Chen,Lora H Rigatti,Caroline G Madigan,Rajiv Dhir,Wade Bushman,Donald B DeFranco,Naoki Yoshimura,Zhou WangPubMed 35361739
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Tested | Tested |
| Rat | Not recommended | Not recommended | Not recommended | Tested | Tested |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 | Notes For unpurified use at 1/500000. |
Species Rat | Dilution info 1/10000 | Notes For unpurified use at 1/500000. |
Species Human | Dilution info 1/10000 | Notes For unpurified use at 1/500000. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/80000 | Notes For unpurified use at 1/100. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/80000 | Notes For unpurified use at 1/100. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Human | Dilution info 1/80000 | Notes For unpurified use at 1/100. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Associated Products
Select an associated product type
7 products for Alternative Product
Target data
Function
CDH1 encodes a protein that is a calcium-dependent cell adhesion molecule, playing a role in cell-cell adhesion, mobility, and proliferation in epithelial cells. It acts as a potent invasive suppressor and serves as a ligand for integrin alpha-E/beta-7. The protein contributes to the sorting of heterogeneous cell types due to its homophilic interactions. E-Cad/CTF2, a product of CDH1, promotes the non-amyloidogenic degradation of Abeta precursors and strongly inhibits the production of APP C99 and C83. Additionally, in the context of microbial infection, it functions as a receptor for Listeria monocytogenes, with internalin A binding to it to promote bacterial uptake. This supplementary information is collated from multiple sources and compiled automatically.
Alternative names
CD324, CDHE, UVO, CDH1, Cadherin-1, CAM 120/80, Epithelial cadherin, Uvomorulin, E-cadherin
Recommended products
Rabbit Recombinant Monoclonal E Cadherin phospho S838 + S840 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 62 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EP913(2)Y
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
E-Cadherin sometimes called CDH1 or Cadherin-1 is a protein that plays a role in cell-to-cell adhesion. This transmembrane protein has a molecular weight of approximately 120 kDa. E-Cadherin is mainly expressed in epithelial tissues of various organs including the skin and gut. Its adhesive function is made possible by its extracellular domain that facilitates homophilic binding between cells contributing to the maintenance of tissue architecture and cellular integrity.
- Biological function summary
E-Cadherin participates in establishing and maintaining adherens junctions which are vital for tissue structure. E-Cadherin operates as a core component of the cadherin-catenin complex which links the protein to the actin cytoskeleton. Through this linkage E-Cadherin plays an important role in signaling pathways that influence cellular growth and differentiation. The protein's ability to mediate intercellular connections also regulates cellular motility and supports basic aspects of cell behavior in epithelial tissues.
- Pathways
E-Cadherin influences both the Wnt signaling pathway and the epithelial-to-mesenchymal transition (EMT). Within the Wnt signaling pathway E-Cadherin partners with β-catenin a significant player in transcription regulation and cell signaling. Disruption in E-Cadherin's adhesive functionality can lead to increased β-catenin availability affecting downstream transcriptional control. In the EMT process E-Cadherin loss characterizes an important step in which cells gain migratory and invasive properties typically seen during metastasis in cancer progression.
- Associated diseases and disorders
E-Cadherin's role is prominent in cancer particularly in the context of gastric and breast cancers. Mutations or altered expression of E-Cadherin lead to diminished cell adhesion promoting tumorigenesis and metastatic spread. In gastric cancer for instance mutated E-Cadherin often associates with loss of epithelial function facilitating cancer cell dissemination. Additionally its loss or dysfunction may correlate with proteins such as β-catenin further impacting cancer progression and pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-phospho-s838-s840-antibody-ep9132y-ab76319--western-blot-img144798.jpg "Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)")
Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
Blocking buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319) at 1/500000 dilution
Lane 1: Human brain lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg
Lane 2: Human brain lysate at 15 µg
Lane 3: Rat brain lysate, membrane treated with Alkaline Phosphatase for 1 hour at 15 µg
Lane 4: Rat brain lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 97 kDa
Observed band size: 145 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-phospho-s838-s840-antibody-ep9132y-ab76319--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118854.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling E Cadherin with Purified ab76319 at 1:80000 dilution (0.009 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-phospho-s838-s840-antibody-ep9132y-ab76319--western-blot-img44022.jpg "Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)")
Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
Blocking buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319) at 1/10000 dilution
All lanes: Mouse brain lysate at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 97 kDa
Observed band size: 145 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-phospho-s838-s840-antibody-ep9132y-ab76319--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img124344.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling E Cadherin with Purified ab76319 at 1:80000 dilution (0.009 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-phospho-s838-s840-antibody-ep9132y-ab76319--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111535.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue sections labeling E Cadherin with Purified ab76319 at 1:80000 dilution (0.009 µg/ml). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-phospho-s838-s840-antibody-ep9132y-ab76319--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44763.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)")
This image is courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)
Unpurified ab76319 staining E Cadherin in mouse skin (pilosebaceous untis) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde, permeabilized with Tween-20 and blocked with 10% normal donkey serum + 1% serum for 40 minutes at room temperature; antigen retrieval was by heat mediation in a citrate buffer, pH 6. Samples were incubated with primary antibody (1/400 in 1% BSA) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319), expandable thumbnail](https://content.abcam.com/products/images/e-cadherin-phospho-s838-s840-antibody-ep9132y-ab76319--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img44763.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E Cadherin (phospho S838 + S840) antibody [EP913(2)Y] (ab76319)")
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