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AB193642

Anti-E. coli ethanolamine ammonia lyase light chain antibody

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Rabbit Polyclonal E. coli ethanolamine ammonia lyase light chain antibody. Suitable for WB and reacts with Bacteria samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Escherichia coli K-12 eutC.

View Alternative Names

b2440, JW2433, eutC, Ethanolamine ammonia-lyase small subunit, EAL small subunit, Ethanolamine ammonia-lyase beta subunit, Ethanolamine ammonia-lyase light chain, Ethanolamine deaminase small subunit

1 Images
Western blot - Anti-E. coli ethanolamine ammonia lyase light chain antibody (AB193642)
  • WB

Supplier Data

Western blot - Anti-E. coli ethanolamine ammonia lyase light chain antibody (AB193642)

All lanes:

Western blot - Anti-E. coli ethanolamine ammonia lyase light chain antibody (ab193642) at 3 µg/mL

Lane 1:

Rosseta bacteria lysate at 80 µg

Lane 2:

Rosseta bacteria lysate at 40 µg

Lane 3:

Rosseta bacteria lysate at 20 µg

Lane 4:

Rosseta bacteria lysate at 10 µg

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/5000 dilution

Predicted band size: 32 kDa

Observed band size: 32 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Bacteria

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Escherichia coli K-12 eutC.

P19636

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
>95%,Protein G purified
Storage buffer
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The E. coli ethanolamine ammonia lyase (EAL) light chain also known as EutC plays a mechanical role in the degradation of ethanolamine. This protein is essential for breaking down ethanolamine to acetaldehyde and ammonia. EAL light chain partners with the heavy chain EutB to form the functional enzyme. The molecular weight of the EAL light chain is approximately 19 kDa. E. coli expresses this protein mostly in environments where ethanolamine and coenzyme B12 (adenosylcobalamin) are available as it requires these components for function.
Biological function summary

The degradation of ethanolamine by the EAL light chain involves a radical-based mechanism. EAL exists as part of a multimeric complex known as the ethanolamine ammonia-lyase complex. This complex facilitates the conversion of ethanolamine into acetaldehyde and ammonia aiding the bacteria's survival and growth. This process is significant because it can utilize ethanolamine from cell membranes as a carbon and nitrogen source giving E. coli a competitive advantage in certain habitats.

Pathways

Ethanolamine ammonia lyase functions in the ethanolamine utilization (eut) pathway. This pathway allows E. coli to metabolize ethanolamine when glucose is scarce. In this pathway ethanolamine with the assistance of coenzyme B12 gets converted to acetaldehyde subsequently entering the central metabolism as a two-carbon unit. The related proteins in this pathway such as EutB EutA and EutN collaborate to ensure the completion of ethanolamine breakdown.

The activity of the ethanolamine ammonia lyase complex has relevance to some bacterial infections. Overexpression of EAL can enhance E. coli's ability to colonize the gut potentially leading to urinary tract infections. Other proteins like EutB and factors in the coenzyme B12 synthesis pathway connect to these disorders by aiding bacterial pathogenesis. Understanding this relationship is key to developing strategies targeting microbial ethanolamine metabolism to mitigate infection risks.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Catalyzes the deamination of various vicinal amino-alcohols to oxo compounds (PubMed : 19762342). Ethanolamine ammonia-lyase (EAL) allows bacteria to utilize ethanolamine as the sole source of nitrogen and carbon in the presence of external vitamin B12. It is spontaneously inactivated by its substrate and reactivated by EutA (PubMed : 15466038). Directly targeted to the BMC. May play a role in bacterial microcompartment (BMC) assembly or maintenance (By similarity).
See full target information eutC
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