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AB35654

Anti-E. coli LPS antibody [2D7/1]

4

(9 Reviews)

|

(82 Publications)

Anti-E. coli LPS antibody [2D7/1] (ab35654) is a mouse monoclonal antibody detecting E. coli LPS in ICC/IF, ELISA. Suitable for Escherichia coli.

- Over 60 publications
- Trusted since 2006

View Alternative Names

Escherichia coli LPS

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-E. coli LPS antibody [2D7/1] (AB35654)
  • ICC/IF

AbReview11942****

Immunocytochemistry/ Immunofluorescence - Anti-E. coli LPS antibody [2D7/1] (AB35654)

ab35654 staining E. coli LPS in mouse hepatocytes (red). Cells were PFA fixed and permeabilized in 0.1% Triton X-100 prior to blocking in SFX Kit blocking agent for 30 minutes at 25°C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 25°C. The secondary antibody was Alexa Fluor® 555 goat anti-mouse, diluted 1/100. Cells were stimulated for 90 minutes with E. coli LPS and then probed with ab35654. The picture was taken on a Leica confocal microscope that pseudo-colors the 555 secondary red. The blue staining was with To-Pro-III Iodide,again, pseudo-colored to be blue on confocal.

This image is courtesy of an anonymous Abreview

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

2D7/1

Isotype

IgG2b

Light chain type

kappa

Carrier free

No

Reacts with

Escherichia coli

Applications

ICC/IF, ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

The J5 mutant of E. coli lacks the enzyme uridine diphosphate glucose 4-epimerase and therefore produces an incomplete LPS, deficient in galactose and all the sugars distal to the central polymers. The J5 mutant has no O-specific chains and its endotoxin remains as the core LPS containing lipid A, N acetyl glucosamine, 2-keto-3-deoxyoctonate, heptose and glucose (a composition similar to that of the Rc strains of Salmonella).

The clone 2D7/1 has also been found to react with K. pneumoniae, S. sonnei and S. typhimurium.

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Reactivity data

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Product details

Product Specifications

Anti-E. coli LPS antibody [2D7/1] (ab35654) is a mouse monoclonal antibody and is validated for use in ELISA, ICC/IF in E. coli samples.
Anti-E. coli LPS antibody [2D7/1] (ab35654) specifically detects E. coli LPS (UniProt ID: ; Molecular weight: kDa) and is sold in 100 µg selling sizes.

Quality and Validation

Abcam's high quality validation processes ensure Anti-E. coli LPS antibody [2D7/1] (ab35654) has high sensitivity and specificity.
Anti-E. coli LPS antibody [2D7/1] (ab35654) has been cited over 64 times in peer reviewed journals and is trusted by the scientific community.
Anti-E. coli LPS antibody [2D7/1] (ab35654) has 8 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information E. coli LPS
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Publications (82)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 16:1652433 PubMed40963610

2025

Intratumoral bacterial load and tertiary lymphoid structure density in hepatocellular carcinoma: association and prognostic significance.

Applications

Unspecified application

Species

Unspecified reactive species

Shen Qu,Weili Jia,Xiaoyan Liu,Qianyun Yao,Chao Chen,Zihao Zhao,Ye Nie,Feng Chang,Zexu Yang,Chaosheng Peng,Yangang Wang,Wenjie Song

Alzheimer's & dementia : the journal of the Alzheimer's Association 21:e70331 PubMed40731189

2025

Gut-derived bacterial vesicles carrying lipopolysaccharide promote microglia-mediated synaptic pruning.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoduo Zhao,Jiayi Yu,Bin Xu,Zhi Xu,Xia Lei,Shilong Han,Shangfei Luo,Can Zhang,Guoping Peng,Jing Li,Jie Yu,Yi Ling,Zhongqin Fan,Wei Mo,Ying Yang,Jing Zhang

Nature communications 16:4357 PubMed40348743

2025

Surface lipoprotein sorting by crosstalk between Lpt and Lol pathways in gram-negative bacteria.

Applications

Unspecified application

Species

Unspecified reactive species

Qingshan Luo,Chengai Wang,Shuai Qiao,Shan Yu,Lianwan Chen,Seonghoon Kim,Kun Wang,Jiangge Zheng,Yong Zhang,Fan Wu,Xiaoguang Lei,Jizhong Lou,Michael Hennig,Wonpil Im,Long Miao,Min Zhou,Weiwei Bei,Yihua Huang

Nature communications 16:607 PubMed39799152

2025

Two-component system GrpP/GrpQ promotes pathogenicity of uropathogenic Escherichia coli CFT073 by upregulating type 1 fimbria.

Applications

Unspecified application

Species

Unspecified reactive species

Xueping Li,Yu Pang,Lingyan Jiang,Le Liu,Jiarui Zhou,Chen Jin,Qian Wang,Hongmin Sun,Qing Li,Zhen Chen,Jingliang Qin,Jianwei Mu,Bin Liu,Qiyue Zhang,Yutao Liu,Lu Feng,Lei Wang

Gut microbes 16:2439534 PubMed39673542

2024

Dietary modulation of gut microbiota affects susceptibility to drug-induced liver injury.

Applications

Unspecified application

Species

Unspecified reactive species

Han Pan,Delei Song,Zhiyi Wang,Xin Yang,Pei Luo,Wei Li,Yan Li,Mengxue Gong,Chenhong Zhang

mSystems 10:e0076524 PubMed39660866

2024

Association of intratumoral microbiome diversity with hepatocellular carcinoma prognosis.

Applications

Unspecified application

Species

Unspecified reactive species

Fengle Jiang,Yuan Dang,Zheting Zhang,Yanan Yan,Yingchao Wang,Yi Chen,Lihong Chen,Jialiang Zhang,Jingfeng Liu,Jianmin Wang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2310236 PubMed39476363

2024

Adipose Tissue-Resident Sphingomonas Paucimobilis Suppresses Adaptive Thermogenesis by Reducing 15-HETE Production and Inhibiting AMPK Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yucheng Zhu,Ruiqi Yang,Zhangchao Deng,Bohua Deng,Kun Zhao,Chen Dai,Gang Wei,YanJiang Wang,Jinshui Zheng,Zhuqing Ren,Wentao Lv,Yingping Xiao,Zhinan Mei,Tongxing Song

Chinese medicine 19:137 PubMed39369234

2024

Proteomic analysis and experimental validation reveal the blood-brain barrier protective of Huanshaodan in the treatment of SAMP8 mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Yunfang Su,Ningning Liu,Pan Wang,Congcong Shang,Ruiqin Sun,Jinlian Ma,Zhonghua Li,Huifen Ma,Yiran Sun,Zijuan Zhang,Junying Song,Zhishen Xie,Jiangyan Xu,Zhenqiang Zhang

Molecular medicine (Cambridge, Mass.) 30:127 PubMed39179968

2024

Caspase-11 signaling promotes damage to hippocampal CA3 to enhance cognitive dysfunction in infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ni Liang,Yi Li,Chuang Yuan,Xiaoli Zhong,Yanliang Yang,Fang Liang,Kai Zhao,Fangfang Yuan,Jian Shi,Erhua Wang,Yanjun Zhong,Guixiang Tian,Ben Lu,Yiting Tang

Microorganisms 12: PubMed39065096

2024

A Photonic Immunosensor Detection Method for Viable and Non-Viable in Water Samples.

Applications

Unspecified application

Species

Unspecified reactive species

Ana Fernández Blanco,Yolanda Moreno,Jorge García-Hernández,Manuel Hernández
View all publications
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