Rabbit Polyclonal e-LOX-3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALOXE3.
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- Frontiers in cell and developmental biology 9:7515932021Oncogenic Activation of YAP Signaling Sensitizes Ferroptosis of Hepatocellular Carcinoma ALOXE3-Mediated Lipid Peroxidation Accumulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifei Qin,Zhuo Pei,Zhuan Feng,Peng Lin,Shijie Wang,Yong Li,Fei Huo,Quancheng Wang,Zhiping Wang,Zhi-Nan Chen,Jiao Wu,Yi-Fei WangPubMed 34977009
- Brain research bulletin 170:81-892021The ALOXE3 gene variants from patients with Dravet syndrome decrease gene expression and enzyme activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMei-Mei Gao,Hao-Ying Huang,Si-Yu Chen,Hui-Ling Tang,Na He,Wen-Cai Feng,Ping Lu,Fei Hu,Hua-Juan Yan,Yue-Sheng LongPubMed 33581311
- Cell death & disease 11:9882020Discovery of a novel ferroptosis inducer-talaroconvolutin A-killing colorectal cancer cells in vitro and in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYong Xia et. al.PubMed 33203867
- Cellular and molecular neurobiology :2020Expression Pattern of ALOXE3 in Mouse Brain Suggests Its Relationship with Seizure Susceptibility.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui-Ling Tang et. al.PubMed 33058074
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 1% BSA- Form
Lyophilized
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ALOXE3. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BYJ1
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/500.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Non-heme iron-containing lipoxygenase which is atypical in that it displays a prominent hydroperoxide isomerase activity and a reduced lipoxygenases activity (PubMed:12881489, PubMed:17045234, PubMed:20921226, PubMed:20923767). The hydroperoxide isomerase activity catalyzes the isomerization of hydroperoxides, derived from arachidonic and linoleic acid by ALOX12B, into hepoxilin-type epoxyalcohols and ketones (PubMed:12881489, PubMed:17045234, PubMed:20923767). In presence of oxygen, oxygenates polyunsaturated fatty acids, including arachidonic acid, to produce fatty acid hydroperoxides (PubMed:20921226). In the skin, acts downstream of ALOX12B on the linoleate moiety of esterified omega-hydroxyacyl-sphingosine (EOS) ceramides to produce an epoxy-ketone derivative, a crucial step in the conjugation of omega-hydroxyceramide to membrane proteins (PubMed:21558561). Therefore plays a crucial role in the synthesis of corneocytes lipid envelope and the establishment of the skin barrier to water loss (PubMed:21558561). In parallel, it may have a signaling function in barrier formation through the production of hepoxilins metabolites (PubMed:21558561). Plays also a role in adipocyte differentiation through hepoxilin A3 and hepoxilin B3 production which in turn activate PPARG (By similarity). Through the production of hepoxilins in the spinal cord, it may regulate inflammatory tactile allodynia (By similarity).
Alternative names
Hydroperoxide isomerase ALOXE3, Epidermis-type lipoxygenase 3, Hydroperoxy dehydratase ALOXE3, Hydroperoxy icosatetraenoate dehydratase, Hydroperoxy icosatetraenoate isomerase, Epidermal LOX-3, e-LOX-3, eLOX-3, ALOXE3
Recommended products
Rabbit Polyclonal e-LOX-3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ALOXE3.
Alternative names
Hydroperoxide isomerase ALOXE3, Epidermis-type lipoxygenase 3, Hydroperoxy dehydratase ALOXE3, Hydroperoxy icosatetraenoate dehydratase, Hydroperoxy icosatetraenoate isomerase, Epidermal LOX-3, e-LOX-3, eLOX-3, ALOXE3
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 1% BSA- Form
Lyophilized
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ALOXE3. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BYJ1
- Purification technique
Affinity purification Immunogen
- Reconstitution
Reconstitute in 200µl Steriled Distilled Water
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The e-LOX-3 also known as Epidermis-type Lipoxygenase 3 is an enzyme that plays a role in the lipoxygenase family. It has an estimated mass of approximately 75 kDa. Researchers find e-LOX-3 expressed in human skin playing a role in the epidermal layer. This enzyme participates in the process of oxidation of polyunsaturated fatty acids. It influences the formation of certain signaling molecules and lipid peroxidation products in the body.
- Biological function summary
Mutations affecting e-LOX-3 disrupt important epidermal barrier functions. e-LOX-3 acts as a component of the complex lipoxygenase pathway involved in metabolizing fatty acids into oxygenated products. This pathway is important for maintaining skin health and homeostasis by contributing to the development and maintenance of the epidermal lipid barrier. e-LOX-3 helps create metabolites that act as signaling molecules in skin differentiation and function.
- Pathways
E-LOX-3 is an important element in the arachidonic acid pathway and the related lipoxygenase pathway. Lipoxygenase enzymes such as 12-LOX and 15-LOX interact with e-LOX-3 in producing bioactive lipids essential for skin health. The enzyme plays a role in converting polyunsaturated fatty acids into hydroxy fatty acids or hydroperoxy derivatives which serve critical functions in inflammation and repair processes within the skin.
- Associated diseases and disorders
Alterations in e-LOX-3 function link to ichthyosis and psoriasis. e-LOX-3 mutations can cause non-bullous congenital ichthyosiform erythroderma (NCIE) characterized by scaly skin due to disrupted lipid metabolism. Its activity impacts other proteins like transglutaminase 1 which is critical in maintaining skin barrier function. Understanding the role of e-LOX-3 in skin conditions provides potential therapeutic targets for treatment strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-e-LOX-3 antibody (ab118470)
All lanes: Western blot - Anti-e-LOX-3 antibody (ab118470) at 1/500 dilution
All lanes: Human fetal lung lysate
Predicted band size: 81 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-e-LOX-3 antibody (ab118470)
Staining of Human e-LOX-3 in a Formalin/PFA-fixed paraffin-embedded section of Human fetal skin using ab118470 at a dilution of 1/100.
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