Goat Polyclonal E-Syt1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ESYT1 aa 1000-1050.
Images
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human ESYT1 aa 1000-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BSJ8
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Monkey | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 3.75 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Cow, Dog, Pig, Monkey, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.50000-3.00000 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Horse, Cow, Dog, Pig, Monkey, Orangutan | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Binds glycerophospholipids in a barrel-like domain and may play a role in cellular lipid transport (By similarity). Binds calcium (via the C2 domains) and translocates to sites of contact between the endoplasmic reticulum and the cell membrane in response to increased cytosolic calcium levels. Helps tether the endoplasmic reticulum to the cell membrane and promotes the formation of appositions between the endoplasmic reticulum and the cell membrane.
Alternative names
FAM62A, KIAA0747, MBC2, ESYT1, Extended synaptotagmin-1, E-Syt1, Membrane-bound C2 domain-containing protein
Recommended products
Goat Polyclonal E-Syt1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ESYT1 aa 1000-1050.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human ESYT1 aa 1000-1050. The exact immunogen used to generate this antibody is proprietary information. Database link Q9BSJ8
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
E-Syt1 also known as Extended Synaptotagmin 1 is a protein encoding a molecule of approximately 129 kDa. This protein plays an essential role in the tethering of the endoplasmic reticulum (ER) to the plasma membrane facilitating lipid transfer between these organelles. It is mostly expressed in the brain and other tissues. E-Syt1 possesses N-terminal transmembrane segments that anchor it within the ER membrane and its C-terminal part reaches out to bind PI(45)P2 at the plasma membrane upon increased cytosolic calcium levels.
- Biological function summary
The Extended Synaptotagmin family which includes E-Syt1 forms a complex known as the E-Syt1/SMP complex which aids in the transfer of lipids for membrane repair and cellular homeostasis. The protein ensures cell membrane integrity by shuttling lipids in response to cellular signals. This mechanism supports cell survival and function especially under stress conditions where membrane damage may occur.
- Pathways
E-Syt1 is integral to phosphoinositide and calcium signaling processes. It forms important interactions within the phosphoinositide metabolism pathway facilitating membrane dynamics. The protein also intersects with pathways involving calcium signaling due to its calcium-binding capacity working alongside proteins like Synaptotagmin to fine-tune cellular responses to fluctuations in calcium levels.
- Associated diseases and disorders
Extended Synaptotagmin 1 has implications in conditions like neurodegenerative diseases and certain cancers. Alterations in E-Syt1 activity or expression levels can contribute to conditions such as Alzheimer’s disease where disrupted lipid transport affects neuronal health. E-Syt1 in tandem with proteins like tau in Alzheimer’s highlights its role in maintaining cellular functions that prevent disease progression. Its involvement in lipid transfer can also relate to cancer pathologies where cell membrane dynamics are important.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-E-Syt1 antibody (ab189199)
All lanes: Western blot - Anti-E-Syt1 antibody (ab189199) at 0.5 µg/mL
All lanes: Human Adipose lysate (in RIPA buffer) at 35 µg
Developed using the ECL technique.
Predicted band size: 123 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E-Syt1 antibody (ab189199)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human placenta tissue labeling E-Syt1 with ab189199 at 3.75 μg/ml.
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Product protocols
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