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AB307277

Anti-E-Tag antibody

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Rabbit Polyclonal E tag antibody. Suitable for ICC/IF and reacts with Tag samples.

View Alternative Names

E epitope tag, GAPVPYPDPLEPR epitope tag, GAPVPYPDPLEPR tag

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-E-Tag antibody (AB307277)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-E-Tag antibody (AB307277)

Immunofluorescent analysis of 4% paraformaldehyde (PFA) - fixed, permeabilized with 0.1% Triton-X-100 in CHO cells transfected with 12-tags constructs (named as CHO-12 tags) labelling E Tag with ab307277 at 1/1000 dilution (1ug/ml), followed by a Donkey Anti-Rabbit IgG (H&L) Alexa Fluor 594 antibody at 1/500 dilution (4ug/ml) (Red). Nucleus was counterstained with DAPI (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-E-Tag antibody (AB307277)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-E-Tag antibody (AB307277)

Immunofluorescent analysis of 4% paraformaldehyde (PFA) - fixed, permeabilized with 0.1% Triton-X-100 in CHO cells transfected with 12-tags constructs (named as CHO-12 tags) labelling E Tag with ab307277 at 1/1000 dilution (1ug/ml), followed by a Donkey Anti-Rabbit IgG (H&L), FITC conjugated secondary antibody at 1/1000 dilution (1 ug/ml) (Green). Nucleus was counterstained with DAPI (Blue). Image also shows parallel staining in parental CHO cell line.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Applications

ICC/IF

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antibody was immunoaffinity purified off a peptide containing immunosorbent.
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information E tag
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Product promise

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