AB264179

Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody

Name: Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179) | Abcam
Brand: Abcam Limited
SKU: AB264179

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(2 Publications)

Rabbit Polyclonal E1 Ubiquitin Activating Enzyme 1/UBA1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human UBA1 aa 1-50.

View Alternative Names

A1S9T, UBE1, UBA1, Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1

2 Images

Supplier Data

Immunoprecipitation - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (AB264179)

UBA1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20% of IP loaded) using ab264179 at 3 μg/mg lysate. Western blot was performed on the immunprecipitate using ab264179 at 1 μg/ml.

Lane 1 : ab264179 in HeLa whole cell lysate.

Lane 2 : Control IgG in HeLa whole cell lysate.

Detected with chemilimuniscence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179)

Predicted band size: 118 kDa

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Supplier Data

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (AB264179)

4-8% SDS-PAGE.

All lanes:

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

HEK-293T whole cell lysate at 50 µg

Lane 5:

NIH/3T3 whole cell lysate at 50 µg

Secondary

All lanes:

Goat anti-Rabbit Light Chain HRP Conjugate

Predicted band size: 118 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human UBA1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P22314

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The E1 Ubiquitin Activating Enzyme 1 also known as UBA1 performs the initial step in the ubiquitination process. This enzyme activates ubiquitin a small protein that tags other proteins for degradation by the proteasome. UBA1 binds to ubiquitin in an ATP-dependent manner facilitating its transfer to E2 ubiquitin-conjugating enzymes. UBA1 has a molecular mass of approximately 117 kDa and shows expression in various tissues particularly in actively dividing cells.

Biological function summary

The enzyme plays an essential role in protein homeostasis by initiating the ubiquitin-proteasome system. UBA1 actively contributes to cellular regulation by targeting damaged or misfolded proteins for degradation thereby maintaining protein quality control. The enzyme is part of a complex alongside other proteins involved in the ubiquitination pathway which include E2 enzymes and E3 ubiquitin ligases that work together to achieve selective protein ubiquitination.

Pathways

E1 Ubiquitin Activating Enzyme 1 functions centrally in the ubiquitin-proteasome pathway a major mechanism governing protein turnover. It cooperates with other enzymes such as E2 and E3 to form a cascade that ensures substrate specificity and timely degradation. The enzyme also connects to the NF-kB signaling pathway where it regulates the stability of inhibitor kB proteins influencing immune responses and inflammation processes.

Mutations or dysregulation of UBA1 contribute to spinal muscular atrophy (SMA) and X-linked spinal muscular atrophy (XL-SMA). These conditions involve motor neuron degeneration linked to the impaired ubiquitin-proteasome function. Additionally alterations in UBA1 activity can impact B-cell-activation through the NF-kB pathway possibly leading to autoimmune disorders. Understanding the interactions of UBA1 with associated proteins provides insight into potential therapeutic targets for treating these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed : 1447181, PubMed : 1606621, PubMed : 33108101). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed : 1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed : 22456334).

See full target information UBA1

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:159 PubMed39901028

2025

Arginine methyltransferase PRMT1 promotes ferroptosis through EGR1/GLS2 axis in sepsis-related acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Min Li,Longhui Hu,Qiao Ke,Zhao Li,Chujun Ruan,Hanjing Lu,Xiaoran Liu

Respiratory research 24:165 PubMed37344798

2023

CircItgb5 promotes synthetic phenotype of pulmonary artery smooth muscle cells via interacting with miR-96-5p and Uba1 in monocrotaline-induced pulmonary arterial hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Su,Huiqi Zhu,Sihao Wang,Yeping Li,Chao Yan,Jiaoyan Wang,Kejing Ying

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody

Immunoprecipitation - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (AB264179)

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (AB264179)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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