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Rabbit Polyclonal E1 Ubiquitin Activating Enzyme 1/UBA1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human UBA1 aa 1-50.

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Images

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (AB264179), expandable thumbnail
  • Immunoprecipitation - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (AB264179), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human UBA1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P22314

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Predicted
Predicted
Cow
Predicted
Predicted
Rabbit
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

2-5 µg

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Rat, Rabbit, Cow

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/2000 - 1/10000

Notes

-

Species

Human

Dilution info

1/2000 - 1/10000

Notes

-

Predicted
Predicted

Species

Rat, Rabbit, Cow

Dilution info

-

Notes

-

Associated Products

Select an associated product type

5 products for Alternative Product

Target data

Function

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1447181, PubMed:1606621, PubMed:33108101). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).

Alternative names

Recommended products

Rabbit Polyclonal E1 Ubiquitin Activating Enzyme 1/UBA1 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human UBA1 aa 1-50.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human UBA1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information. Database link P22314
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The E1 Ubiquitin Activating Enzyme 1 also known as UBA1 performs the initial step in the ubiquitination process. This enzyme activates ubiquitin a small protein that tags other proteins for degradation by the proteasome. UBA1 binds to ubiquitin in an ATP-dependent manner facilitating its transfer to E2 ubiquitin-conjugating enzymes. UBA1 has a molecular mass of approximately 117 kDa and shows expression in various tissues particularly in actively dividing cells.

Biological function summary

The enzyme plays an essential role in protein homeostasis by initiating the ubiquitin-proteasome system. UBA1 actively contributes to cellular regulation by targeting damaged or misfolded proteins for degradation thereby maintaining protein quality control. The enzyme is part of a complex alongside other proteins involved in the ubiquitination pathway which include E2 enzymes and E3 ubiquitin ligases that work together to achieve selective protein ubiquitination.

Pathways

E1 Ubiquitin Activating Enzyme 1 functions centrally in the ubiquitin-proteasome pathway a major mechanism governing protein turnover. It cooperates with other enzymes such as E2 and E3 to form a cascade that ensures substrate specificity and timely degradation. The enzyme also connects to the NF-kB signaling pathway where it regulates the stability of inhibitor kB proteins influencing immune responses and inflammation processes.

Associated diseases and disorders

Mutations or dysregulation of UBA1 contribute to spinal muscular atrophy (SMA) and X-linked spinal muscular atrophy (XL-SMA). These conditions involve motor neuron degeneration linked to the impaired ubiquitin-proteasome function. Additionally alterations in UBA1 activity can impact B-cell-activation through the NF-kB pathway possibly leading to autoimmune disorders. Understanding the interactions of UBA1 with associated proteins provides insight into potential therapeutic targets for treating these diseases.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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2 product images

  • Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179), expandable thumbnail

    Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179)

    4-8% SDS-PAGE.

    All lanes: Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179) at 0.04 µg/mL

    Lane 1: HeLa whole cell lysate at 50 µg

    Lane 2: HeLa whole cell lysate at 15 µg

    Lane 3: HeLa whole cell lysate at 5 µg

    Lane 4: HEK-293T whole cell lysate at 50 µg

    Lane 5: NIH/3T3 whole cell lysate at 50 µg

    Secondary

    All lanes: Goat anti-Rabbit Light Chain HRP Conjugate

    Predicted band size: 118 kDa

    Exposure time: 30s

  • Immunoprecipitation - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179), expandable thumbnail

    Immunoprecipitation - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179)

    UBA1 was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (20% of IP loaded) using ab264179 at 3 μg/mg lysate. Western blot was performed on the immunprecipitate using ab264179 at 1 μg/ml.

    Lane 1: ab264179 in HeLa whole cell lysate.

    Lane 2: Control IgG in HeLa whole cell lysate.

    Detected with chemilimuniscence with exposure time of 30 seconds.

    All lanes: Immunoprecipitation - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab264179)

    Predicted band size: 118 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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