Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (AB34711)

ICC/IF image of ab34711 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34711, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (AB34711)

ab34711 (4µg/ml) staining E1 Ubiquitin activating enzyme in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear and weak cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (AB34711)

This image shows human lung tissue stained with ab34711 at 10μg/ml. In many cells apunctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining.

• WB

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Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (AB34711)

All lanes:

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab34711) at 1 µg/mL

Lane 1:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg/mL

Lane 2:

Human liver tissue lysate - total protein (<a href='/en-us/products/unavailable/human-liver-tissue-lysate-total-protein-ab29889'>ab29889</a>) at 10 µg/mL

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg/mL

Lane 4:

MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg/mL

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 118 kDa

Observed band size: 118 kDa,35 kDa,53 kDa

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Exposure time: 1min