Rabbit Polyclonal E1 Ubiquitin Activating Enzyme 1/UBA1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UBA1.
Images
Publications
Filter by
- Molecular therapy. Nucleic acids 31:466-4812023The SWIB/MDM2 motif of UBE4B activates the p53 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesH Helena Wu,Sarah Leng,Yasser Abuetabh,Consolato Sergi,David D Eisenstat,Roger LengPubMed 36865087
- Cell death & disease 11:5032020Spermidine activates RIP1 deubiquitination to inhibit TNF-α-induced NF-κB/p65 signaling pathway in osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhong Chen et. al.PubMed 32632306
- Chemico-biological interactions 306:70-7720194-PBA prevents diabetic muscle atrophy in rats by modulating ER stress response and ubiquitin-proteasome system.Applications:Unspecified applicationReactive species:Unspecified reactive speciesS Sreenivasa Reddy et. al.PubMed 30980806
- Journal of cellular biochemistry 120:5962-59732018Ubiquitin-proteasome system and ER stress in the brain of diabetic rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarnam Shruthi,S Sreenivasa Reddy,P Swathi Chitra,G Bhanuprakash ReddyPubMed 30317658
- JCI insight 1:e879082016Systemic restoration of UBA1 ameliorates disease in spinal muscular atrophy.
- Cell biochemistry and function 33:525-332015PUGNAc induces protein ubiquitination in C2C12 myotube cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJa-Hye Park,Jeong-Eun Lee,Pyong-Gon Moon,Moon-Chang BaekPubMed 26531776
- PloS one 7:e318582012Functional complexity of the axonal growth cone: a proteomic analysis.Applications:ICC/IF, WBReactive species:RatAdriana Estrada-Bernal,Staci D Sanford,Lucas J Sosa,Glenn C Simon,Kirk C Hansen,Karl H PfenningerPubMed 22384089
- The Biochemical journal 441:927-362011Changes in the ratio of free NEDD8 to ubiquitin triggers NEDDylation by ubiquitin enzymes.Applications:WBReactive species:Unspecified reactive speciesRoland Hjerpe,Yann Thomas,Jesse Chen,Aleksandra Zemla,Siobhan Curran,Natalia Shpiro,Lawrence R Dick,Thimo KurzPubMed 22004789
- FASEB journal : official publication of the Federa 22:2901-112008Protein ubiquitination is modulated by O-GlcNAc glycosylation.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Lyophilized
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human UBA1. Database link P22314
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Dog | Predicted | Predicted | Predicted |
| Rabbit | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/5000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Dog | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2-20 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Dog | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rabbit, Dog | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1447181, PubMed:1606621, PubMed:33108101). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).
Alternative names
A1S9T, UBE1, UBA1, Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1
Recommended products
Rabbit Polyclonal E1 Ubiquitin Activating Enzyme 1/UBA1 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human UBA1.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Lyophilized
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human UBA1. Database link P22314
- Purification technique
- Affinity purification Protein A
- Specificity
A BLAST analysis was used to suggest that this antibody would react with Ubiquitin Activating Enzyme E1 protein from human (100%) rabbit (96%), mouse (95%), rat (95%) and dog (93%) based on a high degree of sequence homology. Cross reactivity against this protein from other sources has not been determined.
- Concentration
- Purification notes
The product was purified from monospecific antiserum by a multi step procedure.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The E1 Ubiquitin Activating Enzyme 1 also known as UBA1 performs the initial step in the ubiquitination process. This enzyme activates ubiquitin a small protein that tags other proteins for degradation by the proteasome. UBA1 binds to ubiquitin in an ATP-dependent manner facilitating its transfer to E2 ubiquitin-conjugating enzymes. UBA1 has a molecular mass of approximately 117 kDa and shows expression in various tissues particularly in actively dividing cells.
- Biological function summary
The enzyme plays an essential role in protein homeostasis by initiating the ubiquitin-proteasome system. UBA1 actively contributes to cellular regulation by targeting damaged or misfolded proteins for degradation thereby maintaining protein quality control. The enzyme is part of a complex alongside other proteins involved in the ubiquitination pathway which include E2 enzymes and E3 ubiquitin ligases that work together to achieve selective protein ubiquitination.
- Pathways
E1 Ubiquitin Activating Enzyme 1 functions centrally in the ubiquitin-proteasome pathway a major mechanism governing protein turnover. It cooperates with other enzymes such as E2 and E3 to form a cascade that ensures substrate specificity and timely degradation. The enzyme also connects to the NF-kB signaling pathway where it regulates the stability of inhibitor kB proteins influencing immune responses and inflammation processes.
- Associated diseases and disorders
Mutations or dysregulation of UBA1 contribute to spinal muscular atrophy (SMA) and X-linked spinal muscular atrophy (XL-SMA). These conditions involve motor neuron degeneration linked to the impaired ubiquitin-proteasome function. Additionally alterations in UBA1 activity can impact B-cell-activation through the NF-kB pathway possibly leading to autoimmune disorders. Understanding the interactions of UBA1 with associated proteins provides insight into potential therapeutic targets for treating these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab34711)
All lanes: Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab34711) at 1 µg/mL
Lane 1: Human brain tissue lysate - total protein (ab29466) at 10 µg/mL
Lane 2: Human liver tissue lysate - total protein (ab29889) at 10 µg/mL
Lane 3: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg/mL
Lane 4: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg/mL
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 118 kDa
Observed band size: 118 kDa, 35 kDa, 53 kDa
Exposure time: 1min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab34711)
This image shows human lung tissue stained with ab34711 at 10μg/ml. In many cells apunctate nuclear staining was observed. Other cells showed both cytoplasmic and nuclear staining.
Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab34711)
ICC/IF image of ab34711 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab34711, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody (ab34711)
ab34711 (4µg/ml) staining E1 Ubiquitin activating enzyme in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is nuclear and weak cytoplasmic staining.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com