AB181225

Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal E1 Ubiquitin Activating Enzyme 1/UBA1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

A1S9T, UBE1, UBA1, Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

Immunofluorescent analysis of acetone-fixed HeLa cells labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixed K562 cells labeling E1 Ubiquitin Activating Enzyme with ab181225 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed byGoat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Supplier Data

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

All lanes:

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225) at 1/10000 dilution

Lane 1:

Human fetal brain tissue lysate at 20 µg

Lane 2:

K562 cell lysate at 20 µg

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 118 kDa

Observed band size: 118 kDa

false

Supplier Data

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

Diluting and blocking buffer : 5% NFDM/TBST 5% NFDM/TBST.

The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 118 kDa,117 kDa

Observed band size: 117 kDa,118 kDa

false

Exposure time: 8s

Lab

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

Blocking and diluting buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (ab181225) at 1/1000 dilution

Lane 1:

GST tagged Recombinant Human UBA7(Ube1L) protein (Full length, 166 KDa)

Lane 2:

GST tagged Recombinant Human UBA1 protein (Full length, 146 KDa)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 118 kDa

Observed band size: 146 kDa,166 kDa

false

Exposure time: 180s

Carrier free

Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14204(B)

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The E1 Ubiquitin Activating Enzyme 1 also known as UBA1 performs the initial step in the ubiquitination process. This enzyme activates ubiquitin a small protein that tags other proteins for degradation by the proteasome. UBA1 binds to ubiquitin in an ATP-dependent manner facilitating its transfer to E2 ubiquitin-conjugating enzymes. UBA1 has a molecular mass of approximately 117 kDa and shows expression in various tissues particularly in actively dividing cells.

Biological function summary

The enzyme plays an essential role in protein homeostasis by initiating the ubiquitin-proteasome system. UBA1 actively contributes to cellular regulation by targeting damaged or misfolded proteins for degradation thereby maintaining protein quality control. The enzyme is part of a complex alongside other proteins involved in the ubiquitination pathway which include E2 enzymes and E3 ubiquitin ligases that work together to achieve selective protein ubiquitination.

Pathways

E1 Ubiquitin Activating Enzyme 1 functions centrally in the ubiquitin-proteasome pathway a major mechanism governing protein turnover. It cooperates with other enzymes such as E2 and E3 to form a cascade that ensures substrate specificity and timely degradation. The enzyme also connects to the NF-kB signaling pathway where it regulates the stability of inhibitor kB proteins influencing immune responses and inflammation processes.

Mutations or dysregulation of UBA1 contribute to spinal muscular atrophy (SMA) and X-linked spinal muscular atrophy (XL-SMA). These conditions involve motor neuron degeneration linked to the impaired ubiquitin-proteasome function. Additionally alterations in UBA1 activity can impact B-cell-activation through the NF-kB pathway possibly leading to autoimmune disorders. Understanding the interactions of UBA1 with associated proteins provides insight into potential therapeutic targets for treating these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed : 1447181, PubMed : 1606621, PubMed : 33108101). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed : 1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed : 22456334).

See full target information UBA1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39684409

2024

Ubiquitin-Activating Enzyme E1 (UBA1) as a Prognostic Biomarker and Therapeutic Target in Breast Cancer: Insights into Immune Infiltration and Functional Implications.

Applications

Unspecified application

Species

Unspecified reactive species

Mingtao Feng,Huanhuan Cui,Sen Li,Liangdong Li,Changshuai Zhou,Lei Chen,Yiqun Cao,Yang Gao,Deheng Li

Nature communications 15:3789 PubMed38710693

2024

A conserved N-terminal motif of CUL3 contributes to assembly and E3 ligase activity of CRL3.

Applications

Unspecified application

Species

Unspecified reactive species

Weize Wang,Ling Liang,Zonglin Dai,Peng Zuo,Shang Yu,Yishuo Lu,Dian Ding,Hongyi Chen,Hui Shan,Yan Jin,Youdong Mao,Yuxin Yin

The EMBO journal 43:1089-1109 PubMed38360992

2024

Structural insights into the ubiquitylation strategy of the oligomeric CRL2 E3 ubiquitin ligase.

Applications

Unspecified application

Species

Unspecified reactive species

Zonglin Dai,Ling Liang,Weize Wang,Peng Zuo,Shang Yu,Yaqi Liu,Xuyang Zhao,Yishuo Lu,Yan Jin,Fangting Zhang,Dian Ding,Weiwei Deng,Yuxin Yin

Nature communications 8:15138 PubMed28469175

2017

Ube2D3 and Ube2N are essential for RIG-I-mediated MAVS aggregation in antiviral innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Yuheng Shi,Bofeng Yuan,Wenting Zhu,Rui Zhang,Lin Li,Xiaojing Hao,She Chen,Fajian Hou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)]

Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

Flow Cytometry (Intracellular) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (AB181225)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise