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Rabbit Recombinant Monoclonal E1 Ubiquitin Activating Enzyme 1/UBA1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples.

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Images

Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (AB250441), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (AB250441), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (AB250441), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (AB250441), expandable thumbnail
  • Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (AB250441), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFWBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Expected

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human, Rat, Mouse
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1447181, PubMed:1606621, PubMed:33108101). Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181). Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).

Alternative names

A1S9T, UBE1, UBA1, Ubiquitin-like modifier-activating enzyme 1, Protein A1S9, Ubiquitin-activating enzyme E1

Recommended products

Rabbit Recombinant Monoclonal E1 Ubiquitin Activating Enzyme 1/UBA1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR14204(B)
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab250441 is the carrier-free version of Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] ab181225.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The E1 Ubiquitin Activating Enzyme 1 also known as UBA1 performs the initial step in the ubiquitination process. This enzyme activates ubiquitin a small protein that tags other proteins for degradation by the proteasome. UBA1 binds to ubiquitin in an ATP-dependent manner facilitating its transfer to E2 ubiquitin-conjugating enzymes. UBA1 has a molecular mass of approximately 117 kDa and shows expression in various tissues particularly in actively dividing cells.

Biological function summary

The enzyme plays an essential role in protein homeostasis by initiating the ubiquitin-proteasome system. UBA1 actively contributes to cellular regulation by targeting damaged or misfolded proteins for degradation thereby maintaining protein quality control. The enzyme is part of a complex alongside other proteins involved in the ubiquitination pathway which include E2 enzymes and E3 ubiquitin ligases that work together to achieve selective protein ubiquitination.

Pathways

E1 Ubiquitin Activating Enzyme 1 functions centrally in the ubiquitin-proteasome pathway a major mechanism governing protein turnover. It cooperates with other enzymes such as E2 and E3 to form a cascade that ensures substrate specificity and timely degradation. The enzyme also connects to the NF-kB signaling pathway where it regulates the stability of inhibitor kB proteins influencing immune responses and inflammation processes.

Associated diseases and disorders

Mutations or dysregulation of UBA1 contribute to spinal muscular atrophy (SMA) and X-linked spinal muscular atrophy (XL-SMA). These conditions involve motor neuron degeneration linked to the impaired ubiquitin-proteasome function. Additionally alterations in UBA1 activity can impact B-cell-activation through the NF-kB pathway possibly leading to autoimmune disorders. Understanding the interactions of UBA1 with associated proteins provides insight into potential therapeutic targets for treating these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441), expandable thumbnail

    Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)

    This data was developed using Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] ab181225, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] ab181225) at 1/10000 dilution

    Lane 1: Human fetal brain tissue lysate at 20 µg

    Lane 2: K562 cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Lane 4: Jurkat cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 118 kDa

    Observed band size: 118 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)

    This data was developed using Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] ab181225, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling E1 Ubiquitin Activating Enzyme with Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] ab181225 at 1/100 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)

    This data was developed using Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] ab181225, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of acetone-fixed HeLa cells labeling E1 Ubiquitin Activating Enzyme with Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] ab181225 at 1/250 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution (red). Counter stained with Dapi (blue).

  • Flow Cytometry (Intracellular) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)

    This data was developed using Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] ab181225, the same antibody clone in a different buffer formulation.

    Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed K562 cells labeling E1 Ubiquitin Activating Enzyme with Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] ab181225 at 1/10 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441), expandable thumbnail

    Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] - BSA and Azide free (ab250441)

    This data was developed using Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] ab181225, the same antibody clone in a different buffer formulation.

    Diluting and blocking buffer: 5% NFDM/TBST 5% NFDM/TBST.

    The lysates were freshly made and used for Western Blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] (Anti-E1 Ubiquitin Activating Enzyme 1/UBA1 antibody [EPR14204(B)] ab181225) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 118 kDa, 117 kDa

    Observed band size: 117 kDa, 118 kDa

    Exposure time: 8s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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