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AB284715

Anti-E1B-AP5 antibody [EP2633Y] - BSA and Azide free

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Rabbit Monoclonal E1B-AP5 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

E1BAP5, HNRPUL1, HNRNPUL1, Heterogeneous nuclear ribonucleoprotein U-like protein 1, Adenovirus early region 1B-associated protein 5, E1B-55 kDa-associated protein 5, E1B-AP5

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1B-AP5 antibody [EP2633Y] - BSA and Azide free (AB284715)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E1B-AP5 antibody [EP2633Y] - BSA and Azide free (AB284715)

This data was developed using ab68480, the same antibody clone in a different buffer formulation.ab68480 at 1/250 dilution staining E1B-AP5 in human brain by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Western blot - Anti-E1B-AP5 antibody [EP2633Y] - BSA and Azide free (AB284715)
  • WB

Unknown

Western blot - Anti-E1B-AP5 antibody [EP2633Y] - BSA and Azide free (AB284715)

This data was developed using ab68480, the same antibody clone in a different buffer formulation.

Lanes 1-3 : Merged signal (red and green). Green - ab68480 observed at 96 kDa. Red - loading control ab8245 observed at 36 kDa.

ab68480 Anti-E1B-AP5 antibody [EP2633Y] was shown to specifically react with E1B-AP5 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266142 (knockout cell lysate ab257993) was used. Wild-type and E1B-AP5 knockout samples were subjected to SDS-PAGE. ab68480 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-E1B-AP5 antibody [EP2633Y] (<a href='/en-us/products/primary-antibodies/e1b-ap5-antibody-ep2633y-ab68480'>ab68480</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

HNRNPUL1 knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human HNRNPUL1 (E1B-AP5) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-hnrnpul1-e1b-ap5-knockout-hek-293t-cell-line-ab266142'>ab266142</a>)

Lane 3:

Raji cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 96 kDa

Observed band size: 96 kDa

false

Western blot - Anti-E1B-AP5 antibody [EP2633Y] - BSA and Azide free (AB284715)
  • WB

Unknown

Western blot - Anti-E1B-AP5 antibody [EP2633Y] - BSA and Azide free (AB284715)

This data was developed using ab68480, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-E1B-AP5 antibody [EP2633Y] (<a href='/en-us/products/primary-antibodies/e1b-ap5-antibody-ep2633y-ab68480'>ab68480</a>) at 1/50000 dilution

All lanes:

HeLa cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit, HRP conjugated at 1/2000 dilution

Predicted band size: 96 kDa

Observed band size: 125 kDa,96 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP2633Y

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." } } }
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Product details

ab284715 is the carrier-free version of ab68480

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The E1B-AP5 protein also known as hnRNPUL1 serves mechanical functions by binding to RNA and DNA and it acts as a regulator in transcription and mRNA processing. This protein weighs approximately 88 kDa. Researchers have identified its presence in the nucleus of different cell types reflecting its central role in nucleic acid metabolism. The protein can also intervene in alternative splicing events and influence mRNA stability making it a significant player within the cell's genetic expression regulation matrix.
Biological function summary

E1B-AP5 integrates into cellular processes as a part of the heterogeneous nuclear ribonucleoproteins complex which facilitates various RNA processing mechanisms. It plays an active role in chromatin structure modulation and RNA transport fostering proper gene expression and maintaining cellular homeostasis. Besides supporting the transcription machinery E1B-AP5 engages in RNA export and surveillance where its presence determines the fate of newly synthesized RNAs.

Pathways

Studies have shown that E1B-AP5 is involved in pathways such as the mRNA surveillance and splicing pathways. It collaborates with proteins like HNRNPA1 and HNRNPC in orchestrating the correct assembly and exportation of RNA transcripts. By participating in these critical pathways E1B-AP5 ensures the fidelity of gene expression and supports the cellular stress response mechanisms contributing to proper cellular function and adaptability.

Abnormalities in E1B-AP5 expression correlate with oncogenic transformations and neurodegenerative conditions. In cancer for example dysregulation of E1B-AP5 affects RNA processing and can lead to tumor development with its interaction noted alongside the protein p53 in the context of cellular proliferation control. Understanding E1B-AP5’s role in these diseases might provide insights into therapeutic targets particularly where RNA processing and tumor suppressor pathways are implicated.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a basic transcriptional regulator. Represses basic transcription driven by several virus and cellular promoters. When associated with BRD7, activates transcription of glucocorticoid-responsive promoter in the absence of ligand-stimulation. Also plays a role in mRNA processing and transport. Binds avidly to poly(G) and poly(C) RNA homopolymers in vitro.
See full target information HNRNPUL1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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