AB978

Anti-E2 tag antibody [3F12]

Lab Essentials
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(7 Publications)

Mouse Monoclonal E2 tag antibody. Suitable for IP, WB, ICC/IF and reacts with Tag samples. Cited in 7 publications.

View Alternative Names

E2 epitope tag, SSTSSDFRDR epitope tag, SSTSSDFRDR tag

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [3F12] (AB978)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [3F12] (AB978)

Staining of transfected COS-7 cells with COX-2 specific antibody (A, B, D) and with E2Tag-specific antibody (3F12)(C).

A - pcDNA3.1 rabbit COX-2,

B - pCG-E2Tag-COX,

C - pCG-COX[E2Tag]

D – neg. control, carrier DNA alone

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3F12

Isotype

IgG1

Carrier free

Applications

ICC/IF, WB, IP

applications

Epitope

GVSSTSSDFRDR(linear)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Tag": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

E2TAG having sequence SSTSSDFRDR or GVSSTSSDFRDR and antibodies specific for the peptide tag are covered by PCT Patent No PCT/EE01/00001 held by Quattromed Ltd. End-user is granted to use the licenced technology solely for internal research purpose only. This antibody should be used in conjunction with the E2 tagging vectors (links to the appropriate datasheet can be found in the Related Products section at the bottom of the datasheet).

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information E2 tag

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 18:755-67 PubMed17182846

2006

Cell fate determination factor DACH1 inhibits c-Jun-induced contact-independent growth.

Applications

Unspecified application

Species

Unspecified reactive species

Kongming Wu,Manran Liu,Anping Li,Howard Donninger,Mahadev Rao,Xuanmao Jiao,Michael P Lisanti,Ales Cvekl,Michael Birrer,Richard G Pestell

Archives of virology 149:1745-59 PubMed15593417

2004

Human papillomavirus type 16 E5 protein colocalizes with the antiapoptotic Bcl-2 protein.

Applications

Unspecified application

Species

Unspecified reactive species

E Auvinen,A Alonso,P Auvinen

Experimental cell research 286:308-20 PubMed12749859

2003

Mouse NIPK interacts with ATF4 and affects its transcriptional activity.

Applications

Unspecified application

Species

Unspecified reactive species

Daima Ord,Tõnis Ord

The Journal of biological chemistry 276:7033-40 PubMed11085996

2000

The basis of prostaglandin synthesis in coral: molecular cloning and expression of a cyclooxygenase from the Arctic soft coral Gersemia fruticosa.

Applications

Unspecified application

Species

Unspecified reactive species

R Koljak,I Järving,R Kurg,W E Boeglin,K Varvas,K Valmsen,M Ustav,A R Brash,N Samel

BioTechniques 28:456-60, 462 PubMed10723557

2000

Monitoring and purification of proteins using bovine papillomavirus E2 epitope tags.

Applications

Unspecified application

Species

Unspecified reactive species

N Kaldalu,D Lepik,A Kristjuhan,M Ustav

Journal of bacteriology 182:1118-26 PubMed10648539

2000

Functional domains of the TOL plasmid transcription factor XylS.

Applications

Unspecified application

Species

Unspecified reactive species

N Kaldalu,U Toots,V de Lorenzo,M Ustav

Journal of virology 73:4670-7 PubMed10233926

1999

Effect of bovine papillomavirus E2 protein-specific monoclonal antibodies on papillomavirus DNA replication.

Applications

Unspecified application

Species

Unspecified reactive species

R Kurg,J Parik,E Juronen,T Sedman,A Abroi,I Liiv,U Langel,M Ustav

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-E2 tag antibody [3F12]

Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [3F12] (AB978)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Applications

Epitope

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (7)

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Product promise