Anti-E2 tag antibody [5E11]

• ICC/IF

AbReview6038****

Immunocytochemistry/ Immunofluorescence - Anti-E2 tag antibody [5E11] (AB977)

ab977 at 1/200 dilution staining human Hela cells by ICC/IF. The HeLa cells were transfected with the fusion DNA construct pQM-NTag/A consisting of E2 tag fused in frame with gene specific cDNA. The cells were fixed with paraformaldehyde and blocked with serum prior to incubation with the antibody for 2 hours. A Cy3 conjugated sheep anti-mouse IgG antibody was used as the secondary.

This image is courtesy of an Abreview submitted by Dr Ashraf Mannan

• WB

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Western blot - Anti-E2 tag antibody [5E11] (AB977)

Cos-7 cell lysates (1μg of total protein per lane) were transfected with pQM vectors encoding two different E2-tagged proteins. Detection is performed with anti-E2Tag antibody (5E11) (1 : 10 000 in TBST) with and without high salt followed by incubation with goat anti-mouse HRP conjugate (1 : 10,000). Cos-7 cell lysates (1µg of total protein per lane) were transfected with pQM vectors encoding two different E2-tagged proteins. Detection is performed with anti-E2Tag antibody (5E11) (1 : 10 000 in TBST) with and without high salt followed by incubation with goat anti-mouse HRP conjugate (1 : 10,000).

All lanes:

Western blot - Anti-E2 tag antibody [5E11] (ab977)

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