Anti-E2F1 antibody [1D12]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [1D12] (AB288369)

IHC image of ab288369 diluted at 1 : 100 and staining in paraffin-embedded human breast cancer performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

• ICC

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Immunocytochemistry - Anti-E2F1 antibody [1D12] (AB288369)

Immunofluorescence staining of HeLa cells with ab288369 at 1 : 50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).

• Flow Cyt

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Flow Cytometry - Anti-E2F1 antibody [1D12] (AB288369)

Overlay histogram showing HeLa cells stained with ab288369 (red line) at 1 : 50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1μg/1 x 10⁶cells) for 1 h at 4°C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30 min at 4°C. Control antibody (green line) was Rabbit IgG (1μg/1 x 10⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-E2F1 antibody [1D12] (AB288369)

IHC image of ab288369 diluted at 1 : 100 and staining in paraffin-embedded human tonsil tissue performed on a Leica Bond™ system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.

• WB

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Western blot - Anti-E2F1 antibody [1D12] (AB288369)

All lanes:

Western blot - Anti-E2F1 antibody [1D12] (ab288369) at 1/2000 dilution

Lane 1:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 2:

PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate

Lane 3:

Rat heart tissue

Secondary

All lanes:

Goat polyclonal to rabbit IgG at 1/50000 dilution

Predicted band size: 47 kDa

Observed band size: 60 kDa

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