AB112580

Anti-E2F1 antibody

(1 Review)

(19 Publications)

Rabbit Polyclonal E2F1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 19 publications.

View Alternative Names

RBBP3, E2F1, Transcription factor E2F1, E2F-1, PBR3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, pRB-binding protein E2F-1, RBAP-1, RBBP-3

1 Images

Lab

Western blot - Anti-E2F1 antibody (AB112580)

Gel type : MOPS

Blocking buffer : 2% BSA block

Loading Control : GAPDH (ab8245 @ 0.1 ug/ml). Secondary : Goat anti-Mouse IgG H&L (IRDye® 680RD)

All lanes:

Western blot - Anti-E2F1 antibody (ab112580) at 1 µg/mL

Lane 1:

MEF1 whole cell lysate at 20 µg

Lane 2:

NIH 3T3 whole cell lysate lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed at 1/10000 dilution

Predicted band size: 47 kDa

Observed band size: 55 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The E2F1 protein also known as E2F transcription factor 1 is an important regulator of the cell cycle. It has a molecular weight of approximately 47 kDa. E2F1 protein is expressed in many tissues with higher expression levels in proliferating cells. It often acts as a transcription factor by binding to specific DNA sequences controlling the transition of cells from G1 to S phase of the cell cycle. This role is vital for the proper progression of the cycle and cellular proliferation.

Biological function summary

E2F1 plays a significant role in cell growth regulation and apoptosis. E2F1 functions within a complex often forming heterodimers with DP proteins. This combination enhances its ability to bind DNA and activate transcription of genes necessary for DNA replication and cell cycle progression. E2F1 also participates in the control of apoptosis by regulating the expression of pro-apoptotic genes balancing cell proliferation and death.

Pathways

E2F1 is deeply embedded in cell cycle regulatory pathways and the p53 signaling pathway. It closely interacts with proteins like RB (Retinoblastoma protein) and p53. RB protein regulates E2F1 activity by controlling its release while p53 helps in mediating the cell's response to DNA damage potentially leading to either cell cycle arrest or apoptosis. These interactions highlight E2F1's role in maintaining cellular homeostasis.

E2F1 is often linked to cancer and neurodegenerative diseases. Overexpression or dysregulation of E2F1 can lead to uncontrolled cell proliferation contributing to oncogenesis. In the context of neurodegenerative diseases E2F1 influences neuronal apoptosis and may be associated with disorders such as Alzheimer's. E2F1 interacts with other proteins such as p53 in cancer where it affects both cell cycle dynamics and apoptosis illustrating its complex involvement in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication (PubMed : 10675335, PubMed : 12717439, PubMed : 17050006, PubMed : 17704056, PubMed : 18625225, PubMed : 28992046). The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase (PubMed : 10675335, PubMed : 12717439, PubMed : 17704056). E2F1 binds preferentially RB1 in a cell-cycle dependent manner (PubMed : 10675335, PubMed : 12717439, PubMed : 17704056). It can mediate both cell proliferation and TP53/p53-dependent apoptosis (PubMed : 8170954). Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed : 20176812). Directly activates transcription of PEG10 (PubMed : 17050006, PubMed : 18625225, PubMed : 28992046). Positively regulates transcription of RRP1B (PubMed : 20040599).

See full target information E2F1

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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Journal of pharmaceutical analysis 15:101228 PubMed41050116

2025

ZFP36 promotes ferroptosis and mitochondrial dysfunction and inhibits malignant progression in osteosarcoma by regulating the E2F1/ATF4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Shiyue Qin,Hongyang Kong,Lei Jiang

Adipocyte 11:572-587 PubMed36093813

2022

Adipose-derived mesenchymal stem cell-secreted extracellular vesicles alleviate non-alcoholic fatty liver disease delivering miR-223-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghui Niu,Ting Wang,Zhiqiang Wang,Feng Wang,Deyu Huang,Huali Sun,Hanyun Liu

Frontiers in pharmacology 13:926211 PubMed35814218

2022

Metformin Improves the Senescence of Renal Tubular Epithelial Cells in a High-Glucose State Through E2F1.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Liang,Zhiyang Li,Zhaowei Feng,Zhiping Yuan,Yunli Dai,Xin Wu,Fan Zhang,Yuanyuan Wang,Yuxia Zhou,Lingling Liu,Mingjun Shi,Ying Xiao,Bing Guo

Analytical cellular pathology (Amsterdam) 2022:7081611 PubMed35592867

2022

Transcription Factor E2F1 Exacerbates Papillary Thyroid Carcinoma Cell Growth and Invasion Upregulation of LINC00152.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Yang,Yong Ying,Xiangtai Zeng,Jiafeng Liu,Yang Xie,Zefu Deng,Zhiqiang Hu,Zanbin Li

Journal of Cancer 13:1664-1678 PubMed35371308

2022

Circular RNA circASPM promotes the progression of glioblastoma by acting as a competing endogenous RNA to regulate miR-130b-3p/E2F1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Dianqi Hou,Zhenlin Wang,Haimeng Li,Juan Liu,Yaohua Liu,Yang Jiang,Meiqing Lou

Bioengineered 13:2360-2370 PubMed35030974

2022

E2F transcription factor 1 (E2F1) enhances the proliferation, invasion and EMT of trophoblast cells by binding to Zinc Finger E-Box Binding Homeobox 1 (ZEB1).

Applications

Unspecified application

Species

Unspecified reactive species

Han Gong,Fan Lu,Xiaoling Zeng,Qing Bai

Molecular therapy. Nucleic acids 27:241-255 PubMed34976441

2022

RNA-binding protein MEX3A controls G1/S transition via regulating the RB/E2F pathway in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yuntan Qiu,Meng Meng,Chuanzhen Cao,Jingyuan Zhang,Xu Cheng,Yongxin Huang,Haotian Cao,Yun Li,Duanqing Tian,Yongsheng Huang,Li Peng,Kaishun Hu,Yin Zhang,Jianyou Liao,Jiehua He,Xiaochun Wang,Daning Lu,Lehang Lin,Xingang Bi,Dong Yin

Molecular therapy : the journal of the American Society of Gene Therapy 30:1275-1287 PubMed34763084

2021

Circular RNA circ-FoxO3 attenuates blood-brain barrier damage by inducing autophagy during ischemia/reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenguo Yang,Cheng Huang,Xueyi Wen,Wenlin Liu,Xiaoxiong Huang,Yufeng Li,Jiankun Zang,Zean Weng,Dan Lu,Chi Kwan Tsang,Keshen Li,Anding Xu

Brain and behavior 11:e2399 PubMed34758200

2021

E2F transcription factor 1 elevates cyclin D1 expression by suppressing transcription of microRNA-107 to augment progression of glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Xie,Shigang Lv,Zhaozhen Wang,Xinzhang Yuan

Bioengineered 12:8020-8030 PubMed34726120

2021

MicroRNA-485-5p inhibits glioblastoma progression by suppressing E2F transcription factor 1 under cisplatin treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Conggang Huang,Lan Ma,Faliang Duan,Ruixue Li,Yanguo Zhang,Yuan Wang,Ming Luo,Zhuqiang He,Zhihua Luo

View all publications

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Product promise

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Anti-E2F1 antibody

Western blot - Anti-E2F1 antibody (AB112580)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (19)

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